Depressed CCL5 Expression in Human Pulmonary Tuberculosis

Lee, Ji-Sook; Kim, Ki Hye; Lee, Da-Youn; Choi, Heung Jai; Lee, Hyemi; Son, Ji Woong; Paik, Tae-Hyun; Jo, Eun-Kyeong

doi:10.4167/jbv.2008.38.3.97

Cited by 3 publications

(2 citation statements)

References 45 publications

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“…RANTES is involved in the selective attraction of memory T cells [37], a signalling molecule that is produced by T cells, macrophages, and a variety of cell types. Lee et al (2008) suggest that altered RANTES production might play a valuable role in immunopathogenesis during TB [38]. We showed significantly lower levels of RANTES in the active TB group after 12 hrs when left unstimulated (p = 0.00 and p = 0.00), SufR stimulated (p = 0.00 and p = 0.00) or BCG stimulated (p = 0.00 and p = 0.00) when compared to QFN pos and QFN neg groups, respectively.…”

Section: Plos One Cells Because Of the High Bacterial Burden (Fig 2c)mentioning

confidence: 99%

Investigating Mycobacterium tuberculosis sufR (rv1460) in vitro and ex vivo expression and immunogenicity

Baatjies,

van Rensberg,

Snyders

et al. 2023

PLoS ONE

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Iron is vital metal for Mycobacterium tuberculosis infection, survival, and persistence within its human host. The mobilization of sulphur (SUF) operon encodes the primary iron-sulphur (Fe-S) biogenesis system in M. tuberculosis and is induced during iron limitation and intracellular growth of M. tuberculosis, pointing to its importance during infection. To study sufR expression at single cell level during intracellular growth of M. tuberculosis a fluorescent reporter was generated by cloning a 123 bp sufR promoter region upstream of a promotorless mcherry gene in an integrating vector. Expression analysis and fluorescence measurements during in vitro culture revealed that the reporter was useful for measuring induction of the promoter but was unable to detect subsequent repression due to the stability of mCherry. During intracellular growth in THP-1 macrophages, increased fluorescence was observed in the strain harbouring the reporter relative to the control strain, however this induction was only observed in a small sub-set of the population. Since SufR levels are predicted to be elevated during infection we hypothesize that it is immunogenic and may induce an immune response in M. tuberculosis infected individuals. The immune response elicited by SufR for both whole blood assay (WBA, a short term 12-hr stimulation to characterise the production of cytokines/growth factors suggestive of an effector response) and lymphocyte proliferation assay (LPA, a longer term 7-day stimulation to see if SufR induces a memory type immune response) were low and did not show a strong immune response for the selected Luminex analytes (MCP-1, RANTES, IL-1b, IL-8, MIP-1b, IFN-g, IL-6 and MMP-9) measured in three clinical groups, namely active TB, QuantiFERON positive (QFN pos) and QFN negative (QFN neg) individuals.

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Section: Plos One Cells Because Of the High Bacterial Burden (Fig 2c)mentioning

confidence: 99%

Investigating Mycobacterium tuberculosis sufR (rv1460) in vitro and ex vivo expression and immunogenicity

Baatjies,

van Rensberg,

Snyders

et al. 2023

PLoS ONE

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“…STAT1 signaling is necessary for the control of Mtb in mice ( Sugawara et al., 2004 ) and polymorphisms in STAT1 have been associated with poor control of mycobacterial diseases ( Dupuis et al., 2001 ; Chapgier et al., 2006 ). CCL5 (RANTES), STAT4, and TNF are generally associated with better control of TB ( Flynn et al., 1995 ; Gómez-Reino et al., 2003 ; Sugawara et al., 2004 ; Askling et al., 2005 ; Stegelmann et al., 2005 ; Lee et al., 2008 ; Vesosky et al., 2010 ). In some reports, CXCL8 (IL8) has been shown to be important for TB control ( O’Kane et al., 2007 ; Krupa et al., 2015 ; Larsen et al., 1995 ), but is also found to be upregulated in granulomas containing excessive neutrophils ( Bergeron et al., 1997 ; Hashemian et al., 2014 ).…”

Section: Activity Of Ldns Isolated From Tb Patientsmentioning

confidence: 99%

Exploring the Role of Low-Density Neutrophils During Mycobacterium tuberculosis Infection

Rankin

Hendrix

Naik

et al. 2022

Front. Cell. Infect. Microbiol.

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Tuberculosis (TB) is caused by infection with the bacterium Mycobacterium tuberculosis (Mtb), which primarily infects the lungs but can also cause extrapulmonary disease. Both the disease outcome and the pathology of TB are driven by the immune response mounted by the host. Infection with Mtb elicits inflammatory host responses that are necessary to control infection, but can also cause extensive tissue damage when in excess, and thus must be precisely balanced. In particular, excessive recruitment of neutrophils to the site of infection has been associated with poor control of Mtb infection, prompting investigations into the roles of neutrophils in TB disease outcomes. Recent studies have revealed that neutrophils can be divided into subpopulations that are differentially abundant in TB disease states, highlighting the potential complexities in determining the roles of neutrophils in Mtb infection. Specifically, neutrophils can be separated into normal (NDN) and low-density neutrophils (LDNs) based on their separation during density gradient centrifugation and surface marker expression. LDNs are present in higher numbers during active TB disease and increase in frequency with disease progression, although their direct contribution to TB is still unknown. In addition, the abundance of LDNs has also been associated with the severity of other lung infections, including COVID-19. In this review, we discuss recent findings regarding the roles of LDNs during lung inflammation, emphasizing their association with TB disease outcomes. This review highlights the importance of future investigations into the relationship between neutrophil diversity and TB disease severity.

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Low transcriptomic of PTPRCv1 and CD3E is an independent predictor of mortality in HIV and tuberculosis co-infected patient

Gebremicael

Gebreegziabxier

Kassa

2022

Sci Rep

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A comprehensive assessment of immunological profiles during HIV-TB co-infection is essential to predict mortality, and facilitate the development of effective diagnostic assays, therapeutic agents, and vaccines. Expression levels of 105 immune-related genes were measured at enrolment and 6th month follow-up from 9 deceased HIV and TB coinfected patients who died between 3 and 7th months follow-up and at enrolment, 6th and 18th month from 18 survived matched controls groups for 2 years. Focused gene expression profiling was assessed from peripheral whole blood using a dual-color Reverse-Transcription Multiplex Ligation-dependent Probe Amplification assay. Eleven of the 105 selected genes were differentially expressed between deceased individuals and survivor-matched controls at baseline. At baseline, IL4δ2 was significantly more highly expressed in the deceased group than survivor matched controls, whereas CD3E, IL7R, PTPRCv1, CCL4, GNLY, BCL2, CCL5, NOD1, TLR3, and NLRP13 had significantly lower expression levels in the deceased group compared to survivor matched controls. At baseline, a non-parametric receiver operator characteristic curve was conducted to determine the prediction of mortality of single genes identified CCL5, PTPRCv1, CD3E, and IL7R with Area under the Curve of 0.86, 0.86, 0.86, and 0.85 respectively. The expression of these genes in the survived control was increased at the end of TB treatment from that at baseline, while decreased in the deceased group. The expression of PTPRCv1, CD3E, CCL5, and IL7R host genes in peripheral blood of patients with TB-HIV coinfected can potentially be used as a predictor of mortality in the Ethiopian setting. Anti-TB treatment might be less likely to restore gene expression in the level expression of the deceased group. Therefore, other new therapeutics that can restore these genes (PTPRCv1, CD3E, IL7R, and CCL5) in the deceased groups at baseline might be needed to save lives.

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Depressed CCL5 Expression in Human Pulmonary Tuberculosis

Cited by 3 publications

References 45 publications

Investigating Mycobacterium tuberculosis sufR (rv1460) in vitro and ex vivo expression and immunogenicity

Investigating Mycobacterium tuberculosis sufR (rv1460) in vitro and ex vivo expression and immunogenicity

Exploring the Role of Low-Density Neutrophils During Mycobacterium tuberculosis Infection

Low transcriptomic of PTPRCv1 and CD3E is an independent predictor of mortality in HIV and tuberculosis co-infected patient

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