Depressed Immune Surveillance Against Cancer: Role of Deficient T Cell: Extracellular Matrix Interactions

Górski, A; Castronovo, Vincenzo; Stepień-Sopniewska, B; Grieb, P; Ryba, Monika; Mrowiec, T; Korczak-Kowalska, G; Wierzbicki, Piotr; Matysiak, Wojciech; Dybowska, B.; Piekarczyk, J; Gawalkiewicz, J.; Kryst, P.; Borkowski, Andrew W.; Tolloczko, T

doi:10.3109/15419069409004441

Cited by 15 publications

(5 citation statements)

References 14 publications

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“…T cells for proliferation and adhesion tests were purified as described previously [30, 31] and were > 95% pure. Proliferative responses to coimmobilized OKT3 antibody and ECM proteins, and adhesion to ECM proteins following short‐term stimulation with phytohaemagglutinin (PHA) were assessed as reported previously [30, 31]. The results were expressed, respectively, as the arithmetic means of [ 3 H]thymidine (cpm) incorporated by triplicate cell cultures and optical density units of crystal violet bound by adhering cells.…”

Section: Methodsmentioning

confidence: 99%

Immune Abnormalities in Aneurysmal Subarachnoid Haemorrhage Patients: Relation to Delayed Cerebral Vasospasm

et al. 2000

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Peripheral blood CD3 , CD19 , CD4 , CD8 and CD45RO mononuclear cell subsets, T-cell proliferative responses to combinations of coimmobilized OKT3 antibody and an ECM protein (collagen I, collagen IV, ®bronectin or elastin), and T-cell adhesion to collagen IV, ®bronectin and elastin were studied in patients with aneurysmal subarachnoid haemorrhage. No signi®cant difference was found in the major lymphocyte subsets between subarachnoid haemorrhage patients receiving no dexamethasone for brain oedema treatment and healthy blood donors. Compared with the latter, both the dexamethasone-untreated and -treated subarachnoid haemorrhage patients showed decreased relative proliferative responses of circulating T cells to OKT3 combinations with collagen IV and ®bronectin, and an increased PHA-activated T-cell adhesion to elastin. CD45RO , CD4 and CD19 peripheral blood cell subsets, CD4 /CD8 cell ratio, PHA-activated T-cell adhesion to ®bronectin and collagen IV, and OKT3-triggered T-cell costimulatory responses to elastin, collagen IV and ®bronectin were signi®cantly higher in subarachnoid haemorrhage patients presenting with delayed cerebral vasospasm (DCV) than in their DCV-free counterparts. The DCV-related differences in circulating lymphocyte subsets showed no apparent relationship to the glucocorticoid treatment, whereas the differences in the other indices were con®ned to the dexamethasone-untreated subarachnoid haemorrhage patients. The above results suggest that the CD4 /CD8 ratio and T cell±ECM interactions play a role in the emergence of subarachnoid haemorrhage/DCV and may represent potential targets for subarachnoid haemorrhage therapy.

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Section: Methodsmentioning

confidence: 99%

Immune Abnormalities in Aneurysmal Subarachnoid Haemorrhage Patients: Relation to Delayed Cerebral Vasospasm

et al. 2000

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“…12 In our hands, stimulation with PHA offers more reproducible and reliable measure of T cell adhesion in comparison with stimulation with PMA. 16 Phorbol esters in 6itro induce a rapid b1 integrin-mediated T cell adhesion to fibronectin and this T cell adhesion is regulated by the activation of protein kinase C, since phytohemaglutinin as a mitogen promote multipoint, also integrin-mediated T cell activation involving tyrosine phosphorylation of focal adhesion kinase. 17,18 Therefore, PHA-activated T cell adhesion to ECM may be more appropriate to mimic placental T cell activation in 6i6o.…”

Section: Discussionmentioning

confidence: 99%

Intravenous Immunoglobulin Therapy Influences T cell Adhesion to Extracellular Matrix in Women with a History of Recurrent Spontaneous Abortions

Jerzak

Górski²,

Rechberger³

2000

American J Rep Immunol

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“…Those severe functional deficits of T-cell-ECM interactions could not be explained by phenotypic deficiencies of T-cell integrin and nonintegrin receptors for ECM proteins. Those novel findings point to the possible role of deficient T-cell-ECM interactions in the disturbances of immune surveillance in patients with cancer (20).…”

Section: Abnormalities Of T-cell-ecm Protein Interactions and Diseasesmentioning

confidence: 90%

Extracellular matrix proteins, regulators of T-cell functions in healthy and diseased individuals

Górski¹,

Kupiec‐Weglinski²

1995

Clin Diagn Lab Immunol

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Depressed Immune Surveillance Against Cancer: Role of Deficient T Cell: Extracellular Matrix Interactions

Cited by 15 publications

References 14 publications

Immune Abnormalities in Aneurysmal Subarachnoid Haemorrhage Patients: Relation to Delayed Cerebral Vasospasm

Immune Abnormalities in Aneurysmal Subarachnoid Haemorrhage Patients: Relation to Delayed Cerebral Vasospasm

Intravenous Immunoglobulin Therapy Influences T cell Adhesion to Extracellular Matrix in Women with a History of Recurrent Spontaneous Abortions

Extracellular matrix proteins, regulators of T-cell functions in healthy and diseased individuals

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