Depression by a Green Tea Extract of Alcohol-Induced Oxidative Stress and Lipogenesis in Rat Liver

Chen, Kuo-Hsin; Li, Ping Chia; Lin, Wei Hsiang; Chien, Chiang Ting; Low, Boon Hua

doi:10.1271/bbb.110163

Cited by 38 publications

(27 citation statements)

References 54 publications

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“…These adipogenic transcription factors enhance the expression of genes such as glucose transporter 4 (GLUT4), ACC, and FAS (21). SREBP-1c is related to the expression of an endogenous PPARγ ligand, and activation of SREBP-1c stimulates expression of FAS, ACC, and FABP (22,23). In our study, SEs significantly down-regulated the expression of genes related to adipogenesis, including C/EBPβ, SREBP-1c, C/EBPα, PPARγ, and adiponectin.…”

Section: Effects Of Ses On Protein Expression Of Genes Involved In Adsupporting

confidence: 56%

Sigumjang (fermented barley bran) water-soluble extracts inhibit the expression of adipogenic and lipogenic regulators in 3T3-L1 adipocytes

Jeong

Lee

Kim

et al. 2016

Food Sci Biotechnol

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prepared from fermented barley bran is a traditional fermented food found only in the Gyeongsang-do area of South Korea. There have been no studies reported to date despite the potential bioactivities of . In this study, the anti-obesity activities of extracts (SEs) during 3T3-L1 differentiation into adipocytes were investigated. SEs inhibited adipocyte differentiation by suppressing the CCAAT/enhancer binding protein-β and sterol regulatory element binding protein-1c expression in the early stage of differentiation, followed by the suppression of the peroxisome proliferator-activated receptor-γ, CCAAT/enhancer binding protein-α, and adiponectin. These changes in adipogenic markers induced inhibition of lipogenesis via down-regulation of mainly fatty acid synthase, acetyl-CoA carboxylase, fatty acid binding protein 4, and perilipin. These results were more significant in the extract of fermented with isolated MFST compared to naturally fermented group. SEs can be considered as a useful material for developing food with health benefits and anti-obesity properties.

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Section: Effects Of Ses On Protein Expression Of Genes Involved In Adsupporting

confidence: 56%

Sigumjang (fermented barley bran) water-soluble extracts inhibit the expression of adipogenic and lipogenic regulators in 3T3-L1 adipocytes

Jeong

Lee

Kim

et al. 2016

Food Sci Biotechnol

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show abstract

“…Both O 2 − and H 2 O 2 belonging to ROS commonly exist in cells. Therefore, O 2 − and H 2 O 2 levels were examined according to the lucigenin-amplified method (57,58). The present study found that MTX did not raise O 2 − levels in the Hep3B cells (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…H 2 O 2 and O 2 − levels were examined by using lucigenin-amplified chemiluminescence according to the lucigenin-amplified method (57,58). In brief, for H 2 O 2 levels, the sample (200 μl) was mixed with 0.2 mmol/l luminol solution (100 μl).…”

Section: Methodsmentioning

confidence: 99%

Vitamin C enhances anticancer activity in methotrexate-treated Hep3B hepatocellular carcinoma cells

et al. 2014

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Methotrexate (MTX) has been widely used for rheumatoid arthritis therapy for a long time. MTX is also used as an anticancer drug for various tumors. However, many studies have shown that high-dose MTX treatment for cancer therapy may cause liver and renal damage. Alhough the mechanisms involved in MTX-induced liver and renal damage require further research, many studies have indicated that MTX-induced cytotoxicity is associated with increases in oxidative stress and caspase activation. In order to reduce MTX-induced side-effects and increase anticancer efficiency, currently, combination treatments of low-dose MTX and other anticancer drugs are considered and applied for various tumor treatments. The present study showed that MTX induces increases in H2O2 levels and caspase-9/-3 activation leading to cell death in hepatocellular carcinoma Hep3B cells. Importantly, this study is the first to demonstrate that vitamin C can efficiently aid low-dose MTX in inducing cell death in Hep3B cells. Therefore, the present study provides a possible powerful therapeutic method for tumors using a combined treatment of vitamin C and low-dose MTX.

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“…H 2 O 2 and O 2 − levels were measured using a lucigenin-amplified chemiluminescence method, as in (56,57). Briefly, 200 μl of cell lysate was mixed with 0.2 mmol/l of luminol solution (100 μl) for the quantification of the H 2 O 2 level, or with 0.1 mmol/l of lucigenin solution (500 μl) for the quantification of the O 2 − level.…”

Section: Methodsmentioning

confidence: 99%

Dual role of acetaminophen in promoting hepatoma cell apoptosis and kidney fibroblast proliferation

Yiang

Chou

et al. 2014

Molecular Medicine Reports

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Acetaminophen (APAP), is a safe analgesic and antipyretic drug at therapeutic dose, and is widely used in the clinic. However, high doses of APAP can induce hepatotoxicity and nephrotoxicity. Most studies have focused on high-dose APAP-induced acute liver and kidney injury. So far, few studies have investigated the effects of the therapeutic dose (1/10 of the high dose) or of the low dose (1/100 of the high dose) of APAP on the cells. The aim of this study was to investigate the cellular effects of therapeutic- or low-dose APAP treatment on hepatoma cells and kidney fibroblasts. As expected, high-dose APAP treatment inhibited while therapeutic and low-dose treatment did not inhibit cell survival of kidney tubular epithelial cells. In addition, therapeutic-dose treatment induced an increase in the H2O2 level, activated the caspase-9/-3 cascade, and induced cell apoptosis of hepatoma cells. Notably, APAP promoted fibroblast proliferation, even at low doses. This study demonstrates that different cellular effects are exerted upon treatment with different APAP concentrations. Our results indicate that treatment with the therapeutic dose of APAP may exert an antitumor activity on hepatoma, while low-dose treatment may be harmful for patients with fibrosis, since it may cause proliferation of fibroblasts.

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Depression by a Green Tea Extract of Alcohol-Induced Oxidative Stress and Lipogenesis in Rat Liver

Cited by 38 publications

References 54 publications

Sigumjang (fermented barley bran) water-soluble extracts inhibit the expression of adipogenic and lipogenic regulators in 3T3-L1 adipocytes

Sigumjang (fermented barley bran) water-soluble extracts inhibit the expression of adipogenic and lipogenic regulators in 3T3-L1 adipocytes

Vitamin C enhances anticancer activity in methotrexate-treated Hep3B hepatocellular carcinoma cells

Dual role of acetaminophen in promoting hepatoma cell apoptosis and kidney fibroblast proliferation

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