Depression of Nitrate and Ammonium Transport in Barley Plants with Diminished Sulphate Status. Evidence of Co-regulation of Nitrogen and Sulphate Intake

Clarkson, David T.; Saker, L. R.; Purves, Judith V.

doi:10.1093/jxb/40.9.953

Cited by 92 publications

(64 citation statements)

References 31 publications

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“…Inoculation with Sp 7 and with 33% fertilizer-N increased bunch yield, while UPMB 10 improved the fruit physical attributes, namely, finger weight, length and diameter and [pulp / peel] ratio, besides inducing early flowering by 3 weeks. The results suggested that PGPR strains Sp 7 and UPMB 10 could be used as bioenhancer and biofertilizer for early, high-yielding and improved banana fruit production in 33% fertilizer-N conditions.…”

Section: Resultsmentioning

confidence: 99%

“…Plant nutrient solution was used according to the modification of Clarkson et al [7]. Two PGPR strains were used in the experiments, namely, Sp 7 (Azospirillum brasilense Azospirillum, a Gram-negative diazotropohic bacteria, which fixes atmospheric nitrogen as free-living or in association with roots of grasses and cereals) and UPMB 10 (Bacillus sphaericus, anaerobic, rod-shaped, endospore-forming, Grampositive bacteria with a nif-gene).…”

Section: Methodsmentioning

confidence: 99%

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High-yielding and quality banana production through plant growth-promoting rhizobacterial (PGPR) inoculation

Shamsuddin

Wahab

Marziah

2005

Fruits

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--Introduction. Rhizobacterial inoculation in low fertilizer-N conditions, viz., 33% fertilizer-N of the total N requirement, could produce similar plant growth to the 100% N-fertilization of banana plantlets grown under hydroponic conditions. Thus, we tested PGPR inoculation in combination with fertilizer-N application to study the role played by strains of rhizobacteria in nutrient accumulation, nitrogen fixation and, consequently, improvement in yield and fruit quality of bananas. Materials and methods. Two PGPR strains were used in the experiments, namely, Sp 7 (Azospirillum brasilense) and UPMB 10 (Bacillus sphaericus). The design of the experiment was completely randomized with three replications. Eight treatments were applied: control without fertilizer-N application (N 0% ) and without PGPR; N 0% + Sp 7 ; N 0% + UPMB 10 ; N 33% without PGPR; N 33% + Sp 7 ; N 33% + UPMB 10 ; N 100% without PGPR and N 100% + UPMB 10 . One tissue-cultured banana (cv. 'Berangan') plantlet was planted in a plastic pot (4 L) for 45 days and thereafter transferred to a larger polyethylene tank (1000 L) until maturity. A 100-mL broth culture of Sp 7 or UPMB 10 was added to the respective tanks after the transplanting process and repeat inoculations were performed monthly. The fruits were harvested at the maturity stage after 80-90 days of flowering. After ripening, yield and fruit quality parameters were assessed. Results. Inoculation with 33% fertilizer-N increased the total nutrient accumulation (N, P, K, Ca and Mg). PGPR inoculation along with 33% fertilizer-N significantly increased the bunch yield and fruit physical attributes, namely, finger weight, length and diameter, and [pulp / peel] ratio, besides inducing early flowering by 3 weeks. Conclusion. The results suggested that PGPR strains Sp 7 and UPMB 10 could be used as bioenhancer and biofertilizer for early, high-yielding and improved banana fruit production in 33% fertilizer-N conditions. Malaysia / Musa / plant nutrition / bacteria / nitrogen fertilizers / growth / yields / fruit quality Production de bananes de qualité, avec de forts rendements, par inoculation avec des rhizobactéries favorisant la croissance des plants (PGPR).Résumé --Introduction. L'inoculation de rhizobactéries en cas de faibles apports d'azote, comme avec une fertilisation à 33 % de N par rapport aux besoins azotés totaux, pourrait permettre d'obtenir une croissance des bananiers équivalente à celle obtenue par fertilisation avec 100 % de N, comme cela a été constaté pour des plantules de bananes développées sous culture hydroponique. Nous avons donc étudié l'inoculation de plants avec des PGPR en combinaison avec une application d'engrais azoté ; le rôle joué par des souches de rhizobactéries sur l'accumulation des éléments nutritifs, la fixation de l'azote et, par conséquent, l'amélioration du rendement et de la qualité des bananes ont été évalués. Matériel et méthodes. Deux souches de PGPR ont été utilisées, à savoir Sp 7 (Azospirillum brasilense) et UPMB 10 (Bacillus sphaericus). L...

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

High-yielding and quality banana production through plant growth-promoting rhizobacterial (PGPR) inoculation

Shamsuddin

Wahab

Marziah

2005

Fruits

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“…In barley it has been results in a substantial increase in respiratory oxygen demonstrated that, under conditions of ammonium consumption (Syrett, 1958;Ohmori ei al., 1984; Di nutrition, \A% of the respiratory energy from roots Martino Rigano et al,, 1986;Turpin et al,, ]990). appears to be associated with the uptake and the Barley seedlings growing with NH4+, but deprived of nitrogen for a given period of time, showed a noticeable increase in their capacity to assimilate NH^^ when it was re-added (Lee & Rudge, 1986;Morgan & Jackson, 1988;Clarkson, Saker & Purves, 1989;Lee et al, 1992;Rideout et al, 1994). Similarly, N-limited unicellular algae not only assimilated NH^* at a greater rate than did Nsufficient algae, but were also able to carry out NHjâ ssimilation independently of photosynthesis.…”

mentioning

confidence: 98%

Ammonium assimilation by young plants of Hordeum vulgare in light and darkness: effects on respiratory oxygen consumption by roots

Rigano

Vona

et al. 1996

New Phytologist

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SUMMARYBarley plants {Hordeum vulgare L.) grown for 10 d in nitrogen-free hydroponic culture, after a rapid initial phase ahsorbed supplied NH,' ai a constant rate of 131 +1'2//mol h"^ g"'f. wt in the light, and at a rate of 13-81 ± 1-6/imol h"'g"'f. wt in darkness. Atnmonium-grown plants assimilated NH^' at a raie of 7-5 ±0'33//.mo! h"'g"'f. wt and at a 5O'\, lower rate in darkness. Nitrogen-free grown plants showed low concentrations of free amino acids in both root and shoot tissues. Supplying NH^" caused an imtnediate increase in the concentration of glutamine in the root tissues of both illuminated and darkened plants over a 120 tnm period. The increase in concentration of glutaminc then exhibited a lap period of 120 min, after wbich it resumed, but to a very small extent. Glutamine also accumulated in shoot tissue of illuminated plants at increasing rates, attaining a concentration which, 8 h after XH^* supply, was 1'61-foId greater than that attained in the roots. In shoots of darkened plants, by contrast, the concentration of glutamine increased slowly and was always smaller than that in the root tissue. Overall formation of glutamine (in sboots and roots) occurred at decreasing rates during the first 4 h, and then at increasing rates. The increase was more pronounced in illuminated plants than in darkened plants. Even 24 h after ^^H^' was supplied, glutamine content in root tissue was lower than that in sboot tissue. However, 48 h later, tbe concentrations of glutamine in root and shoot were similar, attaining values that were almost 47-fold (in root) and 134-fold (in shoot) greater than initial values. Significant levels of asparagine were detected in the root and in the shoot 24 h after adding NH/, These increased further during the succeeding period. Ammonium supply caused a transitory' drop in the concentration of ATP in root tissue, along with noticeable transitory \'ariations in glucose-6-P concentration. A permanent decrease in free glucose concentration was also detected. Addition of NH/ caused 2-and ]-43-fold increases in respiratory oxygen consumption by roots of ijluminated and darkened plants, respectively. Both in the light and in the dark, the root tissue accumulated methylammonium up to a concentration of 55-67 //mol h'' g"^ f. wt. Methylammonium was never found in shoot tissue of either illuminated or darkened plants. Methylammonium stimulated respiration of root barley plants by a factor of 1-2, Regulatory aspects of NHj' metabolism are discussed.

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“…In addition to probable effects of limiting the total nitrogen taken up, the possibility exists for differential effects on NO3-and NH4' uptake. Moreover, the amount and ionic form of nitrogen are significant factors in determining the uptake of other ions such as potassium (23,28), phosphate (25,26), chloride (4,11), and sulfate (2). For example, K+ uptake often is facilitated by NO3- (16) and inhibited by NH4' (27,28), whereas Cl-influx is inhibited by ambient NO3-and by the sum of NO3-and Cl-in the tissue ( (14).…”

mentioning

confidence: 99%

Differential Inhibition by Ferulic Acid of Nitrate and Ammonium Uptake in Zea mays L.

Bergmark¹,

Jackson²,

Volk³

et al. 1992

Plant Physiol.

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The influence of the allelopathic compound ferulic acid (FA) on nitrogen uptake from solutions containing both N03-and NH4+ was examined in 8-day-old nitrogen-depleted com (Zea mays L.) seedlings. Concurrent effects on uptake of Clr and K+ also were assessed. The presence of 250 micromolar FA inhibited the initial (0-1 hours) rate of NO3-uptake and also prevented development of the NO3--inducible accelerated rate. The pattem of recovery when FA was removed was interpreted as indicating a rapid relief of FA-restricted N03-uptake activity, followed by a reinitiation of the induction of that activity. No inhibitiot of NO3-reduction was detected. Ammonium uptake was less sensitive than N03-uptake to inhibition by FA. An inhibition of Cl-uptake occurred as induction of the N03-transport system developed in the absence of FA. Alterations of Cl-uptake in the presence of FA were, therefore, a result of a beneficial effect, because NO3-uptake was restricted, and a direct inhibitory effect. The presence of FA increased the initial net K+ loss from the roots during exposure to the low K, ammonium nitrate uptake solution and delayed the recovery to positive net uptake, but it did not alter the general pattem of the response. The implications of the observations are discussed for growth of plants under natural conditions and cultural practices that foster periodic accumulation of allelopathic substances.A deleterious effect of the phenolic compound FA2 on the uptake of phosphorus and potassium has been observed frequently (6,7,13,21 Little is known about the effect of allelopathic compounds on nitrogen uptake. In addition to probable effects of limiting the total nitrogen taken up, the possibility exists for differential effects on NO3-and NH4' uptake. Moreover, the amount and ionic form of nitrogen are significant factors in determining the uptake of other ions such as potassium (23, 28), phosphate (25, 26), chloride (4, 11), and sulfate (2). For example, K+ uptake often is facilitated by NO3-(16) and inhibited by NH4' (27, 28), whereas Cl-influx is inhibited by ambient NO3-and by the sum of NO3-and Cl-in the tissue (4, 1 1). It is possible, therefore, that effects exerted by allelochemicals on nitrogen uptake may alter the uptake of other ions. Accordingly, the objective of this investigation was to ascertain the inhibitory effect ofa typical phenolic compound, FA, on nitrogen uptake and on concurrent uptake of K+ and C1-. MATERIALS AND METHODS Plant CultureAll experiments were conducted with intact nitrogen-deprived, 8-d-old maize (Zea mays L., Pioneer Hybrid 3369a) seedlings. At this stage of development, the endosperm is largely depleted of nitrogen reserves. Seeds were germinated in darkness at 30C and 98% RH in paper rolls moistened with 0.1 mm CaSO4. After 78 to 82 h, all roots except the primary root were excised. Primary roots of four seedlings (one culture) were threaded through separate holes in the bottom of polyethylene cups (2.5 cm high and 3.5 cm diameter). The seeds within the cups were packed with c...

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Depression of Nitrate and Ammonium Transport in Barley Plants with Diminished Sulphate Status. Evidence of Co-regulation of Nitrogen and Sulphate Intake

Cited by 92 publications

References 31 publications

High-yielding and quality banana production through plant growth-promoting rhizobacterial (PGPR) inoculation

High-yielding and quality banana production through plant growth-promoting rhizobacterial (PGPR) inoculation

Ammonium assimilation by young plants of Hordeum vulgare in light and darkness: effects on respiratory oxygen consumption by roots

Differential Inhibition by Ferulic Acid of Nitrate and Ammonium Uptake in Zea mays L.

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