Depth-specific distribution and importance of nitrite-dependent anaerobic ammonium and methane-oxidising bacteria in an urban wetland

Shen, Li‐dong; Liu, Shuai; He, Zhao; Xu, Liang; Huang, Qian; He, Yuan-Yao; Lou, Liping; Xu, Xiangyang; Zheng, Ping; Hu, Baolan

doi:10.1016/j.soilbio.2015.01.010

Cited by 88 publications

(38 citation statements)

References 60 publications

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“…; Shen et al . ,c). This discrepancy could be explained by the PCR amplification using the specific primers qp1f and qP2r in the previous studies.…”

Section: Discussionmentioning

confidence: 99%

Presence of diverse Candidatus Methylomirabilis oxyfera -like bacteria of NC10 phylum in agricultural soils

Shen

Gao

et al. 2016

J Appl Microbiol

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“…; Shen et al . ,c). This discrepancy could be explained by the PCR amplification using the specific primers qp1f and qP2r in the previous studies.…”

Section: Discussionmentioning

confidence: 99%

Presence of diverse Candidatus Methylomirabilis oxyfera -like bacteria of NC10 phylum in agricultural soils

Shen

Gao

et al. 2016

J Appl Microbiol

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“…Briefly, 50-μl gas samples were withdrawn with a gas-tight glass syringe and injected into a gas chromatograph, while the inlet area was flushed with helium to prevent entry of air. The potential anammox rate and denitrifying rate were calculated from the production of 29 N 2 / 30 N 2 from slurries amended with 15 NO 3 − as previously described (Shen et al 2014(Shen et al , 2015.…”

Section: Isotope Tracer Experimentsmentioning

confidence: 99%

Occurrence and importance of anaerobic ammonium-oxidising bacteria in vegetable soils

Shen

Gao

et al. 2015

Appl Microbiol Biotechnol

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The quantitative importance of anaerobic ammonium oxidation (anammox) has been described in paddy fields, while the presence and importance of anammox in subsurface soil from vegetable fields have not been determined yet. Here, we investigated the occurrence and activity of anammox bacteria in five different types of vegetable fields located in Jiangsu Province, China. Stable isotope experiments confirmed the anammox activity in the examined soils, with the potential rates of 2.1 and 23.2 nmol N2 g(-1) dry soil day(-1), and the anammox accounted for 5.9-20.5% of total soil dinitrogen gas production. It is estimated that a total loss of 7.1-78.2 g N m(-2) year(-1) could be linked to the anammox process in the examined vegetable fields. Phylogenetic analyses showed that multiple co-occurring anammox genera were present in the examined soils, including Candidatus Brocadia, Candidatus Kuenenia, Candidatus Anammoxoglobus and Candidatus Jettenia, and Candidatus Brocadia appeared to be the most common anammox genus. Quantitative PCR further confirmed the presence of anammox bacteria in the examined soils, with the abundance varying from 2.8 × 10(5) to 3.0 × 10(6) copies g(-1) dry soil. Correlation analyses suggested that the soil ammonium concentration had significant influence on the activity and abundance of anammox bacteria in the examined soils. The results of our study showed the presence of diverse anammox bacteria and indicated that the anammox process could serve as an important nitrogen loss pathway in vegetable fields.

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“…The potential n‐damo rates were determined using 13 C stable isotope labelling experiments, as described by Hu and colleagues () and Shen and colleagues (, ). Several negative controls were performed to exclude the possibility of 15 N‐labelled dinitrogen gas production through nitrification, denitrification or anammox and to exclude the possibility of 45 CO 2 production by aerobic methane oxidation.…”

Section: Methodsmentioning

confidence: 99%

“…In soil systems, n‐damo widely occurred in paddyfield surface soils (Wang et al ., ; Shen et al ., ; Zhou et al ., ; Ding et al ., ) and wetland surface soils (Shen et al ., ; Wang et al ., ). In subsurface soil, the occurrence of n‐damo showed discontinuous and depth‐specific distribution (Shen et al ., ), and in the soil/groundwater, ecotone n‐damo bacteria have high abundance and high diversity (Zhou et al ., ). However, there are currently no reports on the occurrence of n‐damo in upland fields.…”

Section: Introductionmentioning

confidence: 99%

Microbial pathways for nitrogen loss in an upland soil

Zhu

Wang

et al. 2018

Environmental Microbiology

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The distribution and importance of anaerobic ammonium oxidation (anammox) and nitrite-dependent anaerobic methane oxidation (n-damo) have been identified in aquatic ecosystems; their role in agricultural upland soils however has not yet been well investigated. In this study, we examined spatio-temporal distributions of anammox and n-damo bacteria in soil profiles (300 cm depth) from an agricultural upland. Monitoring nitrogen (N) conversion activity using isotope-tracing techniques over the course of one year showed denitrification (99.0% N-loss in the winter and 85.0% N-loss in the summer) predominated over anammox (1.0% N-loss in the winter and 14.4% N-loss in the summer) and n-damo (0.6% N-loss in the winter) in surface soils (0-20 cm). While below 20 cm depth, N-loss was dominated by anammox (79.4 ± 14.3% in the winter and 65.4 ± 12.5% in the summer) and n-damo was not detected. Phylogenetic analysis showed that Candidatus Brocadia anammoxidans dominated the anammox community in the surface soil and Candidatus Brocadia fulgida dominated below 20 cm depth. Dissimilatory nitrate reduction to ammonium (DNRA), another nitrite reduction process, was found to play a limited role (4.9 ± 3.5%) in the surface soil compared with denitrification; below 80 cm DNRA rates were much higher than rates of anammox and denitrification. Ammonium oxidation was the main source of NO2- above 80 cm (70.9 ± 23.3%), the key influencing factor on anammox rates, and nitrate reduction (100%) was the main NO2- source below 80 cm. Considering the anammox, n-damo and denitrification rates as a whole in the sampled soil profile, denitrification is still the main N-loss process in upland soils.

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Depth-specific distribution and importance of nitrite-dependent anaerobic ammonium and methane-oxidising bacteria in an urban wetland

Cited by 88 publications

References 60 publications

Presence of diverse Candidatus Methylomirabilis oxyfera -like bacteria of NC10 phylum in agricultural soils

Presence of diverse Candidatus Methylomirabilis oxyfera -like bacteria of NC10 phylum in agricultural soils

Occurrence and importance of anaerobic ammonium-oxidising bacteria in vegetable soils

Microbial pathways for nitrogen loss in an upland soil

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