Der Biosyntheseweg der RNS-Ribose in Hydrogenomonas eutropha Stamm H 16 und Pseudomonas facilis

Bowien, Botho; Schlegel, H. G.

doi:10.1007/bf00409291

Cited by 10 publications

(5 citation statements)

References 33 publications

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“…In gluconate-grown wild-type and mutant cells, the activities of phosphoglucose isomerase and glucose-6-phosphate dehydrogenase which are needed for the rechanneling of carbon into the Entner-Doudoroff pathway were at a basal repressed level. This confirms previous data (4,6). However, the growth of the PGM mutants on fructose was also significantly slower than that of the wild type, indicating a limiting flow of carbon through the recycling sequence.…”

Section: Discussionsupporting

confidence: 91%

“…Autotrophic carbon dioxide assimilation in this organism involves the reactions of the Calvin cycle (8). Fructose and gluconate are metabolized exclusively via the Entner-Doudoroff pathway since A. eutrophus lacks detectable phosphofructokinase (EC 2.7.1.11) and gluconate 6-phosphate dehydrogenase (EC 1.1.1.44) activities (4,6,14). The products of that pathway are pyruvate and glyceraldehyde 3-phosphate.…”

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confidence: 99%

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Effect of phosphoglycerate mutase deficiency on heterotrophic and autotrophic carbon metabolism of Alcaligenes eutrophus

Reutz¹,

Schobert²,

Bowien³

1982

J Bacteriol

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Mutants of Alcaligenes eutrophus were isolated on the basis of their inability to grow on succinate as the sole source of carbon and energy. The mutants also failed to grow on other gluconeogenic substrates, including pyruvate, acetate, and citrate. Simultaneously, they had lost their capability for autotrophic growth. The mutants grew, but slower than the wild type, on fructose or gluconate. Growth retardation on gluconate was more pronounced. The mutants lacked phosphoglycerate mutase activity, and spontaneous revertants of normal growth phenotype had regained the activity. The physiological characteristics of the mutants indicate the role of phosphoglycerate mutase in heterotrophic and autotrophic carbon metabolism of A. eutrophus. Although the enzyme is necessary for gluconeogenesis during heterotrophic growth on three- or four-carbon substrates, its glycolytic function is not essential for the catabolism of fructose or gluconate via the Entner-Doudoroff pathway. The enzyme is required during autotrophic growth as a catalyst in the biosynthetic route leading from glycerate 3-phosphate to pyruvate. It is suggested that the mutants accomplish the complete degradation of fructose and gluconate mutase lesion. The catabolically produced triose phosphates are converted to fructose 6-phosphate which is rechanneled into the Entner-Doudoroff pathway. This carbon recycling mechanism operates less effectively in mutant cells growing on gluconate.

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Section: Discussionsupporting

confidence: 91%

mentioning

confidence: 99%

Effect of phosphoglycerate mutase deficiency on heterotrophic and autotrophic carbon metabolism of Alcaligenes eutrophus

Reutz¹,

Schobert²,

Bowien³

1982

J Bacteriol

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“…Phosphogluconate is also an important intermediate in the heterotrophic metabolism of the hydrogen bacteria. This intermediate is catabolized through the Entner-Doudoroff path in these bacteria (28,71,122). Thus the levels of "free" phosphogluconate in the cell may reflect the function of this path and inhibit the Calvin 295 VOL 37, 1973 on September 26, 2020 by guest http://mmbr.asm.org/ Downloaded from cycle at RuDP carboxylase, an enzyme present at high levels in addition to phosphoribulokinase in sugar-grown cells (143).…”

Section: Regulation Of the Calvin Cycle In Bacteriamentioning

confidence: 99%

Autotrophic CO2 assimilation and the evolution of ribulose diphosphate carboxylase.

McFadden¹

1973

Bacteriological Reviews

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“…When growing lithoautotrophically with hydrogen or organoautotrophically with formate as an energy source, the facultative chemoautotroph Alcaligenes eutrophus assimilates CO2 via the reactions of the Calvin carbon reduction cycle (7). In strain H16, genes encoding enzymes of this cycle (cfx genes) are organized in two large, highly homologous cfx operons.…”

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confidence: 99%

The Calvin cycle enzyme pentose-5-phosphate 3-epimerase is encoded within the cfx operons of the chemoautotroph Alcaligenes eutrophus

et al. 1992

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Several genes (cfir genes) encoding Calvin cycle enzymes in Akaligenes eutrophus are organized in two highly homologous operons comprising at least 11 kb. One cfxr operon is located on the chromosome; the other is located on megaplasmid pHG1 of the organism (B. Bowien, U. Windhovel, J.-G. Yoo, R. Bednarski, and B. Kusian, FEMS Microbiol. Rev. 87:445-450, 1990 When growing lithoautotrophically with hydrogen or organoautotrophically with formate as an energy source, the facultative chemoautotroph Alcaligenes eutrophus assimilates CO2 via the reactions of the Calvin carbon reduction cycle (7). In strain H16, genes encoding enzymes of this cycle (cfx genes) are organized in two large, highly homologous cfx operons. One copy of the operon is located on the chromosome; the other is located on megaplasmid pHG1 adjacent to the hydrogenase gene cluster (9,12,20). Both operons, which possibly originate from a gene duplication event, are functional and expressed simultaneously. Each of them comprises at least 11 kb (48). The two promoterproximal genes, cfxL and cfxS, encode the L and S subunits, respectively, of the C02-fixing enzyme of the Calvin cycle, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), while the gene coding for glyceraldehyde-3-phosphate dehydrogenase (GAP), cfxG, is the most promoter-distal gene so far identified. Also located within the operons are the genes for fructose-1,6-bisphosphatase/sedoheptulose-1,7-bisphosphatase (FBP) (cfrF), phosphoribulokinase (PRK) (cfxP), and transketolase (TK) (cfrl) (Fig.

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Der Biosyntheseweg der RNS-Ribose in Hydrogenomonas eutropha Stamm H 16 und Pseudomonas facilis

Cited by 10 publications

References 33 publications

Effect of phosphoglycerate mutase deficiency on heterotrophic and autotrophic carbon metabolism of Alcaligenes eutrophus

Effect of phosphoglycerate mutase deficiency on heterotrophic and autotrophic carbon metabolism of Alcaligenes eutrophus

Autotrophic CO2 assimilation and the evolution of ribulose diphosphate carboxylase.

The Calvin cycle enzyme pentose-5-phosphate 3-epimerase is encoded within the cfx operons of the chemoautotroph Alcaligenes eutrophus

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