Der Einfluß von Atropin auf die durch intestinale Hormone induzierte Insulinsekretion des Menschen

Abstract

and Conclusions. In 35 metabolically normal subjects the effect of i.v.secretin and eholecystokinin-panereozymin (CCK/PZ) on the endocrine and exoerine pancreatic function was investigated, before and after atropine. In addition, the effect of oral, intravenous and intraduodenal administration of glucose and amino acids on blood sugar and free fatty acids before and after the injection of atropine was studied.The secretin stimulated endocrine and exoerine pancreas was not affected by atropine. However, atropine inhibited the ecbolic and endocrine pancreatic function after stimulation with CCK/PZ. Therefore, the effect of i.v. CCK/PZ seems to be mediated by the cholinergic system, or even a "neuro-hormonal" system which acts synergically or additively.No influence of atropine on the insulin secretion induced by i.v. glucose or amino acids was observed. On the other hand, atropine inhibited the beta-cytotropic effect of glucose and amino acids after oral or intraduodenal administration.These findings indicate that the release of these intestinal hormones is dependent on the cholinergic or parasympathetic system. Zusammen]assung und SchIufl/olgerung. Bei 35 steffweehselgesunden freiwilligenProbanden wurde vor sowie nach Atropingabe die Wirkung der intestinalen Hormone Sekretin und Choleoystekinin-Pankreozymin (CCK-PZ) auf die endokrine und exokrine Pankreasfunktion untersueht. Aul~erdem wurde die Wirkung yon intra-ven6s und oral bzw. intraduodenal verabreichter Glucose und Aminos~iuren auf die * Mit Unterstiitzung dureh die Deutsche Forschungsgemeinschaft. Wesentliche Teile der Arbeit wurden am KongreB der Deutschen und Schweizerisehen Gesellsehaft fiir Verdauungs-und Stoffwechselkrankheiten (Stuttgart, 7.--9. 10. 1971) vorgetragen.Abki~rzungen: Cholecystokinin-Pankreozymin (CCK-PZ), immunologisch meBbares Insulin (IMI), Blutzueker (BZ), freie Fetts~uren (FFS).

Invited commentary

“…In order for a hormone to be accepted as a gastrointestinal insulinotropic hormone, several major criteria must be satisfied: (a) the hormone must be present in the gastrointestinal tract in man; (b) it must be available in purified form for study; (c) it must be insulinotropic when given in physiologic doses; (d) nutrients known to evoke enteric augmentation of insulin secretion must stimulate the release of the hormone. Certain minor criteria should also be fulfilled, namely, secretion of the hormone should be blunted by atropine since Raptus et al [7] clearly demonstrated attenuation of the enteroinsular axis by this drug, and it should be secreted in normal or excessive amounts in maturity-onset diabetics in response to oral glucose or a mixed meal. Most of the known gastrointestinal hormones fail to meet every criterion.…”

Atropine-Induced Inhibition of the Enhanced CCK Release Observed in Alcoholic Dogs

“…2). Recent studies on CCK release from the intestinal mucosa have established that it is partially mediated by cholinergic nerves as described for gastrin, while secretin release seems to be independent of the nervous system (1,3,5,10). The purpose of this study is to evaluate if the postulated increased release of CCK observed in chronic alcoholic dogs is mediated by cholinergic nerves.…”