Der Einfluss von Cobalt auf die Erythropoiese der Ratte

Abstract

Tribukait, B. Der Einfluss von Cobalt auf die Erythropoiese der Ratte. Acta physiol. scand. 1963. 58. 101–110. — The development of polycythemia in rats, treated with cobalt, was studied. Cobalt was injected intraperitoneally 6 times a week in increasing doses of 0.4, 0.6 and 0.8 mg/100 g body weight during 21, 22 and 45 days respectively. The total amount of hemoglobin, hemoglobin concentration, hematocrite, reticulocytes and blood volume were determined. Hemin, tagged in vivo with C14‐2‐glycine, was isolated from 0.1–0.2 ml blood samples and the specific activity was measured. The hemoglobin concentration, hematocrite and reticulocytes increased significantly during treatment with 0.4 mg cobalt, but the total hemoglobin was unchanged. There were no signs of hemolysis as evaluated from the specific hemin activity. During treatment with 0.6 and 0.8 mg cobalt the relative blood values increased further, and also the total hemoglobin increased. The hemoglobin concentration reached values of 20 g/100 ml of blood and the total hemoglobin increased from 2 to 3 g. The blood volume was unchanged. The reticulocytes reached a maximum value after about 40 days treatment and thereafter decreased to normal concentrations despite the increasing total hemoglobin. The degree of this polycythemia was similar to that one developed at an altitude of about 4000 m.