Der Nachweis von Naphthol-AS-D-Chloracetat-Esterase in normalen basophilen Granulopoiesezellen

Parwaresch, M. R.; Dannenberg, K.; Leder, L. D.

doi:10.1007/bf01484408

Cited by 12 publications

(4 citation statements)

References 13 publications

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“…Moreover, the mucosal mast cells, whether located in the epithelium or in the lamina propria, exhibited a positive naphthol-AS-D chloroacetate esterase activity. It is well es tablished by numerous reports that normal mature human blood basophils lack this esterase activity [Parwaresch et al, 1970;Parwaresch, 1976].…”

Section: Discussionmentioning

confidence: 99%

Intraepithelial Migration of Nasal Mucosal Mast Cells in Hay Fever

Enerbäck

Pipkorn

Granérus³

1986

Int Arch Allergy Immunol

154

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Mast cells were studied by light microscopy in mucosal imprints and in biopsies of nasal mucosa of 12 birch pollen allergic individuals before and during the pollen season, using techniques optimized for the demonstration of mucosal mast cells. We also measured the histamine content of nasal mucosa, whole blood and plasma, and counted the numbers of circulating blood basophils. Before the pollen season the nasal mucosa was found to contain many mast cells located in the mucosal connective tissue stroma, and very few cells with basophilic and metachromatic granules were found in mucosal imprints. During the pollen season there was a redistribution of mast cells into the epithelium, many such cells now being recovered in mucosal imprints. The total number of mucosal mast cells counted in tissue sections did not change significantly with the onset of the pollen season, suggesting a redistribution of mucosal mast cells by migration. Judged by morphologic appearance and naphthol-AS-D chloroacetate esterase activity, the intraepithelial mast cells found in tissue sections had rather the properties of tissue mast cells than of blod basophils, and only a few of the basophilic cells of the imprints had a morphology compatible with blood basophils. The histamine content of the mucosa, as well as histamine levels of whole blood and plasma, and circulating blood basophil numbers did not change significantly in relation to the pollen season. These findings suggest that an intraepithelial migration of mucosal mast cells is part of the allergic mucosal response. This reaction resembles the nematode response of certain rodents and may explain how contact is established between mucosal allergens and effector cells when the allergic reaction is initiated.

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Section: Discussionmentioning

confidence: 99%

Intraepithelial Migration of Nasal Mucosal Mast Cells in Hay Fever

Enerbäck

Pipkorn

Granérus³

1986

Int Arch Allergy Immunol

154

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“…This finding has been claimed useful in differentiating mast cells from basophils, since the latter show no reactivity at all (Langner & Steigleder, 1966), or only weak positivity (Yam et al, 1971), with the same substrate. However, Parwaresch et al (1970) state that the reaction may be strongly positive in basophil precursors. These results provide further support for the hypothesis of a common differentiating pathway for the early precursors of the basophil and mast cell line, so that a distinctive cytochemical pattern becomes apparent and sharp enough only in the more advanced stages of maturation.…”

Section: Discussionmentioning

confidence: 99%

Cytobiological and Clinical Aspects of Tissue Mast Cell Leukaemia

et al. 1980

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The authors describe the results of a series of cytochemical, autoradiographic, cytophotometric and immunological investigations carried out in a case of tissue mast cell leukaemia. Leukaemic mast cells showed certain distinctive cytochemical features, amongst which an intense periodic acid-Schiff (PAS) reaction, sensitive to amylase digestion, strong naphthol AS-D chloroacetate esterase (NASDCE), intense lactate dehydrogenase (LD) activity. Proliferative activity, determined autoradiographically with 3H-dT, was considerably low and was mainly confined to the larger cells. Also uridine and leucine incorporation were markedly reduced. Microdensitometry disclosed that the mast cell population was mainly arrested in the G1 phase. Because of previous attempts to destroy selectively neoplastic tissue mast cells with sheep antihuman IgE serum, a search for surface bound IgE was carried out, but gave a negative result. Possible therapeutic approaches are considered in the light of previous clinical experience and on the basis of the results of the kinetic and metabolic studies.

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“…In other words, the enzyme activity closely corresponded to the various developmental stages of the basophils. This could be objectivated by cytophotometrical investigations [15].…”

Section: Resultsmentioning

confidence: 99%

On the Origin of Human Basophilic Granulocytes

Parwaresch¹,

Leder²,

Dannenberg³

1971

Acta Haematol

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By a combined application of toluidine blue stain and naphthol AS-D chloroacetate esterase reaction on normal bone marrow smears metachromic and promyelocytic granules were demonstrated on the same cell. Precursors of basophils contain NASDCl-esterase activity, the intensity of which depended on the maturity of the cells. All transitional cells were found between non metachromatic promyelocytes with strong NASDCl-esterase activity and completely enzymatically negative metachromatic segmented basophils. It is concluded that basophilic granulocytes like monocytes, neutrophils, and eosinophils derive from non specific promyelocytes. Evidence has been presented for the transition of promyelocytic granules to specific basophilic granules. Our results confirm the dualistic view of the cytogenesis of leukocytes

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Der Nachweis von Naphthol-AS-D-Chloracetat-Esterase in normalen basophilen Granulopoiesezellen

Cited by 12 publications

References 13 publications

Intraepithelial Migration of Nasal Mucosal Mast Cells in Hay Fever

Intraepithelial Migration of Nasal Mucosal Mast Cells in Hay Fever

Cytobiological and Clinical Aspects of Tissue Mast Cell Leukaemia

On the Origin of Human Basophilic Granulocytes

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