Der Pflanzenkrebs und sein Erreger, Pseudomonas tumefaciens

Stapp, C.; Bortels, H.

doi:10.1007/bf02146551

Cited by 10 publications

(4 citation statements)

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“…24I. E. HUNTER AND E. D. DELAMATER Feulgen-positiv-e bodies located at the peripherv of the spore have been described for various members of the genus Bacillus by the followinig investigators: Pietschmann anid Rippel (1932), Milovidov (1935), Stille (1937), Schaede (1939), Piekarski (1940), Stapp (1942), Robinow (1945), and Delaporte (1948). Stille claimed that in old spores (from 120-day-old cultures) the niucleus took on a central position.…”

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confidence: 99%

Observations on the Nuclear Cytology of Spore Germination in Bacillus Megaterium

Hunter

DeLamater

1952

J Bacteriol

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Spore germination has generally been recognized to consist of a swelling of the spore followed by a splitting of the spore coat in a characteristic manner. Less often an absorption of the spore coat has been described. This process is accompanied by a decrease in refringence and an increase in stainability. Considerable difference of opinion is apparent, however, concerning the existence, form, and location of a spore nucleus and its activity during germination. The followving brief historical review is concerned only with those investigations which deal with the internal structure of the resting and germinating spore. The reader is referred to the original papers for more detail and to the review of Knaysi (1948) for other aspects of spore germination. Meyer (1897, 1899) was unable to observe a nucleus in the resting spore. However, in swollen spores in the process of preparing for germination, a distinct peripherally located nucleus was observed. In the young bacillus, following germination, one to two nuclei were seen. The spore was regarded by Preisz (1919) as consisting of a strongly refractile "shiny body" made up of reserve material, surrounded by a thin layer of protoplasm and a spore coat. Apparently no nucleus was visible until germination commenced. At this time a round or elongated nucleus appeared between the equatorial side of the shiny body and the spore coat. During the course of germination the nucleus often swelled at the periphery of the spore. The "shiny body" became more stainable. Eventually the spore took on a homogeneous appearance, and the nucleus and the "shiny body" could no longer be distinguished. The young bacillus, as it emerged from the spore coat, contained one or two nuclei. Badian (1933, 1935) was able to stain spores of Bacillus mycoides, Bacillus subtilis, and Bacillus megaterium only after germination had commenced, at which time a central chromatinic rod could be observed. This rod took on a transverse position and divided longitudinally into two daughter "chromosomes". Two types of spores were recognized by Allen, Appleby, and Wolf (1939). The first type was considered to be "haploid" and showed no internal structure either in the resting state or during germination. The second type was considered to be "diploid" and showed, according to these authors, nuclear figures suggesting meiosis during germination.

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confidence: 99%

Observations on the Nuclear Cytology of Spore Germination in Bacillus Megaterium

Hunter

DeLamater

1952

J Bacteriol

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“…Esdrancc and Location of the Bacteria: The path of ciitrnnce of c~o\v11-gall organisms iiito the host in sucli a way that iiifectioii is illduced appears to be tlii*ough wounds of o m type or anotlicr ( Kaiifield, 1928 ;Miincie, 1926;Patty, 1930; Riker, 1 9 2 3~ ; Robinson and Walkden, 1923 ; Smith, 1916e ;and Stapp, 1933). Several workers iiicluding Riker, and Robinson aiid Walkden, have found that bacteria traveled some distalice in the tracheae, but did not induce cell stimulation uiilcss released into the parenchymatous tissue.…”

Section: Anatomymentioning

confidence: 99%

“…He reported curing crown galls on Pelargonium by negating the effect of the mitogenetic radiation with a copper spiral, isolated by ebony, and placed around the diseased plants at the level of the galls. Various workers (Rivera, 1928b;Stapp, 1933;Stapp and Bortels, 1933) have repeated this experiment. None secured Lakhovsky's results.…”

Section: Radiatiortmentioning

confidence: 99%

“…Likewise, cultures of P. tumefacierzs were uninjured on agar (1925) or in broth (19273). However, negative results were obtained with x-ray treatments (including 600 r, 90 kv., no filter for thirty minutes) in two separate studies (Stapp, 1933;Stapp and Bortels, 1933) that were designed to repeat Rivera's work. Such results suggest withholding acceptance of the positive results reported untiI the evidence in their favor is improved.…”

Section: Radiatiortmentioning

confidence: 99%

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