Derangements of pyruvate dehydrogenase in circulating lymphocytes of NIDDM patients and their healthy offspring

Mostert, M.; Rabbone, Ivana; Piccinini, Marco; Curto, M; Vai, Sergio; Musso, Alessandro; Rinaudo, M.

doi:10.1007/bf03343603

Cited by 11 publications

(10 citation statements)

References 41 publications

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“…This conclusion is consistent with the reports of reduced PD activity in T2D subjects and their healthy children (43). This conclusion is consistent with the reports of reduced PD activity in T2D subjects and their healthy children (43).…”

Section: Glycolysis Glucose Oxidation and Pyruvate Dehydrogenasesupporting

confidence: 93%

Glucose Metabolism Gone Wrong

Dods¹

2013

Understanding Diabetes

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Section: Glycolysis Glucose Oxidation and Pyruvate Dehydrogenasesupporting

confidence: 93%

Glucose Metabolism Gone Wrong

Dods¹

2013

Understanding Diabetes

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“…Clinical observations and laboratory findings suggest that lymphocytes may function abnormally in DM [ 5 , 28 , 37 , 40 ]. An increased susceptibility to infections is a well-known complication of a poorly controlled diabetic state [ 18 , 28 , 39 , 41 ]. In diabetic patients, infections take a more severe course and complications occur more often.…”

Section: Introductionmentioning

confidence: 99%

Glucose transport in human peripheral blood lymphocytes influenced by type 2 diabetes mellitus

Piątkiewicz

Czech

Tatoń

2007

Arch. Immunol. Ther. Exp.

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Introduction: The objective of this study was to evaluate glucose transport into lymphocytes in healthy subjects and patients with type 2 diabetes mellitus (DM) treated either with diet only or with insulin and to propose peripheral blood lymphocytes as a convenient model for cellular glucose transport studies. Materials and Methods: Sixty subjects with type 2 DM, 30 treated with diet only and 30 with insulin, were investigated. Thirty healthy subjects matched for age, weight, and sex served as a control group. Deoxy-D-glucose, 2-[ 3 H(G)] transport was studied in isolated peripheral blood lymphocytes. Expression of glucose transporters was ascertained by immunocytochemical identification and by Western blotting. Results: In lymphocytes from the control group, deoxy-D-glucose uptake increased gradually with the duration of the experiment. In diabetics treated with insulin, the maximal increase in deoxy-D-glucose uptake was observed after 30 min of the investigation, followed by a plateau phase. In diabetics treated with diet, deoxy-D-glucose uptake increased slowly during the first 30 min. The presence of GLUT1 and GLUT3 in lymphocytes was confirmed in this study. Conclusions: Glucose transport into lymphocytes is altered in type 2 DM. In lymphocytes from diabetics, the dynamics of deoxy-D-glucose uptake significantly differed from that in healthy subjects. There was also a significant difference between the diabetic groups, representing different modes of therapy and stages of the disease. Glucose transport into lymphocytes is apparently influenced by DM as well as by the mode of therapy. We suggest that peripheral blood lymphocytes may become a promising model for studies on glucose transport in diabetes.

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“…In a series of seven subsequent reactions, two CO 2 molecules are split off, regenerating oxaloacetate (151). Exercise can increase the activity of pyruvate dehydrogenase (152), and pyruvate dehydrogenase activity is less responsive to insulin stimulation both in patients with diabetes and in their offspring (153). Because phosphorylated compounds are charged, most do not cross membranes, and pyruvate dehydrogenase activity remains within the mitochondria.…”

Section: Description Of the Pathwaysmentioning

confidence: 99%

The Cellular Fate of Glucose and Its Relevance in Type 2 Diabetes

et al. 2004

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Type 2 diabetes is a complex disorder with diminished insulin secretion and insulin action contributing to the hyperglycemia and wide range of metabolic defects that underlie the disease. The contribution of glucose metabolic pathways per se in the pathogenesis of the disease remains unclear. The cellular fate of glucose begins with glucose transport and phosphorylation. Subsequent pathways of glucose utilization include aerobic and anaerobic glycolysis, glycogen formation, and conversion to other intermediates in the hexose phosphate or hexosamine biosynthesis pathways. Abnormalities in each pathway may occur in diabetic subjects; however, it is unclear whether perturbations in these may lead to diabetes or are a consequence of the multiple metabolic abnormalities found in the disease. This review is focused on the cellular fate of glucose and relevance to human type 2 diabetes. (Endocrine Reviews 25: 2004)

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Derangements of pyruvate dehydrogenase in circulating lymphocytes of NIDDM patients and their healthy offspring

Cited by 11 publications

References 41 publications

Glucose Metabolism Gone Wrong

Glucose Metabolism Gone Wrong

Glucose transport in human peripheral blood lymphocytes influenced by type 2 diabetes mellitus

The Cellular Fate of Glucose and Its Relevance in Type 2 Diabetes

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