Deregulated expression of Kruppel-like factors in acute myeloid leukemia

Humbert, Magali; Halter, Veronika; Shan, Deborah; Laedrach, Judith; Leibundgut, Elisabeth Oppliger; Baerlocher, Gabriela M.; Tobler, Andreas; Fey, Martin F.; Tschan, Mario P.

doi:10.1016/j.leukres.2011.03.010

Cited by 54 publications

(60 citation statements)

References 25 publications

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“…This preferential effect of 5Aza in the LCA may be due to the following reasons: (a) 5Aza in blood is in direct contact with the target cell types, such as arterial endothelium and circulating leukocytes. (b) Arterial regions exposed to d-flow have high endothelial permeability to blood-borne macromolecules, including anti-miRNAs as demonstrated by our group previously (19), albumin (53), and LDL (54). For a similar reason, 5Aza may be preferentially taken up by the endothelial cells in d-flow regions.…”

Section: Figurementioning

confidence: 68%

“…Mice were sacrificed by CO2 inhalation and were subsequently perfused with saline containing heparin for 5 minutes. Total carotid intimal RNA or gDNA was isolated by flushing of the RCA and LCA with QIAzol (QIAGEN) or Buffer AL (QIAGEN), respectively, by our method that we have demonstrated to be endothelial-enriched without any significant contamination of smooth muscle cells and leukocytes (19,48,49).…”

Section: Methodsmentioning

confidence: 99%

“…We used 2 different ApoE -/-mouse models to study the role of DNMT in atherosclerosis: the acute partial carotid ligation model with Western diet, which rapidly develops atherosclerosis within 3 weeks (49), and the conventional Western diet-fed model that chronically develops atherosclerosis in 3 months (19,50). In the carotid ligation model, the LCA rapidly developed atherosclerosis within 3 weeks ( Figure 3A), and this was inhibited by 5Aza treatment in a dose-dependent manner with 0.2 mg/kg/d being the lowest effective dose ( Figure 3, A and B).…”

Section: Dnmt1 Inhibition In Endothelial Cells By 5aza or Sirna Inhibmentioning

confidence: 99%

“…Interestingly, Klf3 is suppressed in acute myeloid leukemia (AML), and 5Aza is used to treat this disease, although it has not yet been shown whether 5Aza directly targets Klf3. AML is also treated with all-trans retinoic acid, and, also interestingly, this drug was shown to rescue Klf3 expression by an unknown mechanism (19). Since all-trans retinoic acid is known to influence DNA methylation of a small number of specific genes (70), it would be interesting to test whether Klf3 is regulated by this mechanism in AML.…”

Section: Figurementioning

confidence: 99%

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Flow-dependent epigenetic DNA methylation regulates endothelial gene expression and atherosclerosis

Dunn¹,

Qiu²,

Kim³

et al. 2014

J. Clin. Invest.

264

234

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Section: Figurementioning

confidence: 68%

Section: Methodsmentioning

confidence: 99%

Section: Dnmt1 Inhibition In Endothelial Cells By 5aza or Sirna Inhibmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

See 2 more Smart Citations

Flow-dependent epigenetic DNA methylation regulates endothelial gene expression and atherosclerosis

Dunn¹,

Qiu²,

Kim³

et al. 2014

J. Clin. Invest.

264

234

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“…Accumulated evidence has suggested that KLFs play a critical role in tumorigenesis [65][66][67]. For example, KLF2 has been shown to exert cell growth-inhibitory, proapoptotic and anti-angiogenic functions.…”

Section: Kruppel-like Factor 2(klf2)mentioning

confidence: 99%

Untitled

2014

Oncotarget

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ABSTRACT:FBW7 (F-box and WD repeat domain-containing 7) or Fbxw7 is a tumor suppressor, which promotes the ubiquitination and subsequent degradation of numerous oncoproteins including Mcl-1, Cyclin E, Notch, c-Jun, and c-Myc. In turn, FBW7 is regulated by multiple upstream factors including p53, C/EBP-δ, EBP2, Pin1, Hes-5 and Numb4 as well as by microRNAs such as miR-223, miR-27a, miR-25, and miR-129-5p. Given that the Fbw7 tumor suppressor is frequently inactivated or deleted in various human cancers, targeting FBW7 regulators is a promising anticancer therapeutic strategy.

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Expression of human Krüppel‐like factor 3 in peripheral blood as a promising biomarker for acute leukemia

Yan

Liu

et al. 2020

Cancer Medicine

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Background: Universal gene targets are in persistent demand by real-time quantitative polymerase chain reaction (RT-qPCR)-based methods in acute leukemia (AL) diagnosis and monitoring. Human Krüppel-like factor 3 (hKLF3), a newly cloned human transcription factor, has proved to be a regulator of hematopoiesis. Methods: Sanger sequencing was performed in bone marrow (BM) samples from 17 AL patients for mutations in hKLF3 coding exons. hKLF3 expression in peripheral blood (PB) and BM samples from 45 AL patients was dynamically detected by RT-qPCR. PB samples from 31 healthy donors were tested as normal controls. Results: No mutation was sequenced in hKLF3 coding exons. hKLF3 expression in PB of AL was significantly lower than that in healthy donors [0.30 (0.02-1.07) vs 1.18 (0.62-3.37), P < .0001]. Primary acute myeloid leukemia (AML) exhibited the least expression values compared with secondary AML and acute lymphoblastic leukemia. Receiver operating characteristic (ROC) analyses suggested that hKLF3expression in PB was a good marker for AML diagnosis with an AUC of 0.99 (95% CI 0.98-1.00) and an optimum cutoff value of 0.67 (sensitivity 93.94% and specificity 93.55%). hKLF3 expression was upregulated significantly when AML patients acquired morphological complete remission (CR), and the level of hKLF3 seemed to be higher in patients with deeper CR than in patients with minimal residual disease (MRD). Paired PB and BM samples showed highly consistent alteration in hKLF3 expression (r = .6533, P = .001). Besides, a significantly converse correlation between decreased hKLF3 expression in PB and markers for leukemic load was observed. Conclusions: hKLF3 expression in PB may act as a potential marker for AL diagnosis and monitoring. K E Y W O R D Sacute leukemia, biomarker, diagnosis, human krüppel-like factor 3, minimal residual disease 2804 | YAN et Al.

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Deregulated expression of Kruppel-like factors in acute myeloid leukemia

Cited by 54 publications

References 25 publications

Flow-dependent epigenetic DNA methylation regulates endothelial gene expression and atherosclerosis

Flow-dependent epigenetic DNA methylation regulates endothelial gene expression and atherosclerosis

Untitled

Expression of human Krüppel‐like factor 3 in peripheral blood as a promising biomarker for acute leukemia

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