Dereplication by HPLC‐DAD‐ESI‐MS/MS and Screening for Biological Activities of <i>Byrsonima</i> Species (Malpighiaceae)

Fraige, Karina; Dametto, Alessandra Cristina; Zeraik, Maria Luiza; Freitas, Larissa; Saraiva, Amanda Correia; Medeiros, Alexandra Ivo de; Castro‐Gamboa, Ian; Cavalheiro, Alberto José; Silva, Dulce Helena Siqueira; Lopes, Norberto Peporine; Bolzani, Vanderlan S.

doi:10.1002/pca.2734

Cited by 35 publications

(33 citation statements)

References 32 publications

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“…Extracts were analyzed, using six different complementary assays, for their radical-scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), superoxide anion (O 2 − ) and nitric oxide (NO) radicals, for their ferric reducing antioxidant power (FRAP) and the capacity to inhibit lipid peroxidation by β -carotene bleaching test (BCB). Then, the phytochemical profile was performed by LC-ESI-MS/MS analysis and the identification and quantification of polyphenols were achieved using commercially available standards [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity and Phytochemical Characterization of Senecio clivicolus Wedd.

et al. 2018

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Antioxidant phytochemicals play a key role in oxidative stress control and in the prevention of related disorders, such as premature aging, degenerative diseases, diabetes, and cancer. The aim of this study was to investigate the potential antioxidant activity and the phytochemical profile of Senecio clivicolus Wedd., a perennial shrub, belonging to the Asteraceae family. Despite the wide interest of this family, this specie has not been investigated yet. S. clivicolus aerial parts were extracted with 96% ethanol. Then, the ethanol extract was fractionated by liquid/liquid extraction using an increasing solvents polarity. Total polyphenol and terpenoid contents were measured. Moreover, the antioxidant activity was evaluated by six different complementary in vitro assays. The Relative Antioxidant Capacity Index (RACI) was used to compare data obtained by different tests. The sample showing the highest RACI was subjected to characterization and quantitation of its phenolic composition using LC-MS/MS analysis. The ethyl acetate fraction, investigated by LC-MS/MS analysis, showed 30 compounds, most of them are chlorogenic acid and flavonoid derivatives. To the best of our knowledge, this is the first report about the evaluation of antioxidant activity and phytochemical profile of S. clivicolus, underlying the importance of this species as a source of health-promoting phytochemicals.

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Section: Introductionmentioning

confidence: 99%

Antioxidant Activity and Phytochemical Characterization of Senecio clivicolus Wedd.

et al. 2018

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“…The reference hydromethanol extract was chosen to do the analysis for its better compatibility with the MS system. The compounds 1 and 2 were observed only in positive mode, and the compounds 3 – 9 in negative mode, as tentatively identified previously (see Supporting Information, Table S2) . The identification of the compounds was carried out on the basis of heuristic filters.…”

Section: Resultsmentioning

confidence: 76%

“…Antioxidant activity for Byrsonima species has been described in the literature and associated with the presence of phenolic compounds [20,28,29,31,32].…”

Section: Evaluation Of the Influence Of Nades On Antioxidant Activitymentioning

confidence: 99%

Using natural deep eutectic solvents for the extraction of metabolites in Byrsonima intermedia leaves

Fraige

Arrua

Sutton

et al. 2018

J of Separation Science

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Natural deep eutectic solvents have been used as an alternative to organic solvents for the extraction of plants metabolites, allowing for the extraction of compounds of different polarities, while being inexpensive, non‐toxic, and easy to prepare. This work presents the comparison of the chromatographic profiles by high‐performance liquid chromatography with diode‐array detection obtained from Byrsonima intermedia (Malpighiaceae) using five choline chloride‐based natural deep eutectic solvents, in addition to the most used traditional extraction solvents, methanol/water 7:3 and ethanol/water 7:3 v/v. A reference extract was used to tentatively identify compounds by high‐performance liquid chromatography with tandem mass spectrometry. The water content appeared to be important for the extraction efficiency and the mixture choline chloride/glycerol was shown to be the best candidate for efficiently extracting this matrix when compared with the traditional extraction media in addition to being far greener as shown by the environmental analysis tool. Seven phenolic compounds (digalloyl quinic acid, proanthocyanidin dimer, galloylproanthocyanidin dimer, quercetin‐O‐hexoside, galloyl quercetin hexoside, quercetin‐O‐pentoside, and galloyl quercetin pentoside) were tentatively identified in all extracts. Moreover, the influence of these solvents on the antioxidant activity of the extracts was studied and the results for choline chloride/glycerol extracts were very similar to that of the traditional extraction solvents.

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“…Recent study showed that extract of several species of Byrsonima spp. (Malpighiaceae) in different concentrations (12.5 and 50 μg/mL) were not cytotoxic against RAW 264-7 cells (Fraige et al 2018). In 2016, Düsman et al evaluated the cytotoxicity of the aqueous extract from Malpighia glabra linnaeus fruits in vivo and in vitro in Wistar rats, and against bone marrow derived cells from these animals.…”

Section: Discussionmentioning

confidence: 99%

Saline extract from Malpighia emarginata DC leaves showed higher polyphenol presence, antioxidant and antifungal activity and promoted cell proliferation in mice splenocytes.

Barros

Barboza

Ramos

et al. 2019

An. Acad. Bras. Ciênc.

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Currently, the research of new natural compounds with biological potential demonstrates great ethnopharmacological importance. In this study, we evaluated the biological properties promoted by saline extract from Malpighia emarginata DC leaves, whose objective is to evaluate the antioxidant, antimicrobial and cytotoxicity potential. Phytochemical characterization was performed by UPlC-MS chromatography to identify the chemical compounds. For the antioxidant potential, DPPH, ATT and FRAP methods were used. The antibacterial and antifungal tests were performed evaluating the MIC50, MIC90, CMB and CMF parameters. Moreover, antibiofilm action was evaluated. Cytotoxicity and proliferation were performed using splenocytes from Balb/c mice and were evaluated by cytometry. We found a list of phenolic compounds among other bioactive compounds in the M. emarginata saline extract. In addition, higher antioxidant profile and antifungal activity against different strains of Candida spp. was promoted by the saline extract. Splenocytes showed greater cell viability (more than 90%) and showed higher proliferate index in 24 and 48 hours of incubation with the extract. Saline extract from Malpighia emarginata DC has potential action like antioxidant and antifungal agent without promote animal cell damage.

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Dereplication by HPLC‐DAD‐ESI‐MS/MS and Screening for Biological Activities of Byrsonima Species (Malpighiaceae)

Cited by 35 publications

References 32 publications

Antioxidant Activity and Phytochemical Characterization of Senecio clivicolus Wedd.

Antioxidant Activity and Phytochemical Characterization of Senecio clivicolus Wedd.

Using natural deep eutectic solvents for the extraction of metabolites in Byrsonima intermedia leaves

Saline extract from Malpighia emarginata DC leaves showed higher polyphenol presence, antioxidant and antifungal activity and promoted cell proliferation in mice splenocytes.

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