Derivation and Characterization of Canine Embryonic Stem Cell Lines with In Vitro and In Vivo Differentiation Potential

Vaags, Andrea K.; Rosic-Kablar, Suzana; Gartley, Cathy; Zheng, Yan Zhen; Chesney, Alden; Villagómez, D.A.F.; Kruth, Stephen A.; Hough, Margaret R.

doi:10.1634/stemcells.2008-0433

Cited by 73 publications

(67 citation statements)

References 81 publications

(96 reference statements)

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“…Cells derived from plated EBs on day 20 postdifferentiation were analyzed and found to be positive for the presence of cell derivatives from the 3 germ layers, including b-III neuron-specific tubulin (TUJ1) for the ectoderm, vimentin for the mesoderm, and alpha-fetoprotein (AFP) for the endoderm (Fig. 4B) [5,33]. Using qRT-PCR, we also found that differentiated ciPSCs silenced the canine OCT4 and NANOG (P < 0.05; Fig.…”

Section: In Vitro Differentiationmentioning

confidence: 84%

“…Approximately 58% of dog genetic diseases resemble the specific human diseases caused by mutations in the same gene [17,18]. Also, dogs share a variety of biochemical and physiological characteristics with humans; their physiologies, disease presentations, and clinical responses often parallel those of humans better than do those of their rodent counterparts [5,17]. This underscores the dog's importance as a reliable preclinical model for testing the feasibility of regenerative medicine and tissue engineering approaches to treat its own diseases and those of man.…”

Section: Introductionmentioning

confidence: 99%

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Generation of Leukemia Inhibitory Factor and Basic Fibroblast Growth Factor-Dependent Induced Pluripotent Stem Cells from Canine Adult Somatic Cells

Luo

Suhr

Chang

et al. 2011

Stem Cells and Development

119

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For more than thirty years, the dog has been used as a model for human diseases. Despite efforts made to develop canine embryonic stem cells, success has been elusive. Here, we report the generation of canine induced pluripotent stem cells (ciPSCs) from canine adult fibroblasts, which we accomplished by introducing human OCT4, SOX2, c-MYC, and KLF4. The ciPSCs expressed critical pluripotency markers and showed evidence of silencing the viral vectors and normal karyotypes. Microsatellite analysis indicated that the ciPSCs showed the same profile as the donor fibroblasts but differed from cells taken from other dogs. Under culture conditions favoring differentiation, the ciPSCs could form cell derivatives from the ectoderm, mesoderm, and endoderm. Further, the ciPSCs required leukemia inhibitory factor and basic fibroblast growth factor to survive, proliferate, and maintain pluripotency. Our results demonstrate an efficient method for deriving canine pluripotent stem cells, providing a powerful platform for the development of new models for regenerative medicine, as well as for the study of the onset, progression, and treatment of human and canine genetic diseases.

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Section: In Vitro Differentiationmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Generation of Leukemia Inhibitory Factor and Basic Fibroblast Growth Factor-Dependent Induced Pluripotent Stem Cells from Canine Adult Somatic Cells

Luo

Suhr

Chang

et al. 2011

Stem Cells and Development

119

View full text Add to dashboard Cite

show abstract

“…Since then, induced pluripotent stem cells (iPSCs) or iPSC-like cells have been derived from different species, including human [11], rhesus monkey [12], rat [13], pig [14,15], dog [16,17], rabbit [18], marmoset [19], sheep [20][21][22], and more recently, horse [23] and cow [24]. Transgene-mediated reprogramming offers distinct technical advantages over other established reprogramming techniques, and the resulting cell lines are functionally comparable to ESCs [25].…”

Section: Introductionmentioning

confidence: 99%

Derivation and Characterization of Induced Pluripotent Stem Cells from Equine Fibroblasts

Breton

Sharma

Diaz

et al. 2013

Stem Cells and Development

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Pluripotent stem cells offer unprecedented potential not only for human medicine but also for veterinary medicine, particularly in relation to the horse. Induced pluripotent stem cells (iPSCs) are particularly promising, as they are functionally similar to embryonic stem cells and can be generated in vitro in a patient-specific manner. In this study, we report the generation of equine iPSCs from skin fibroblasts obtained from a foal and reprogrammed using viral vectors coding for murine Oct4, Sox2, c-Myc, and Klf4 sequences. The reprogrammed cell lines were morphologically similar to iPSCs reported from other species and could be stably maintained over more than 30 passages. Immunostaining and polymerase chain reaction analyses revealed that these cell lines expressed an array of endogenous markers associated with pluripotency, including OCT4, SOX2, NANOG, REX1, LIN28, SSEA1, SSEA4, and TRA1-60. Furthermore, under the appropriate conditions, the equine iPSCs readily formed embryoid bodies and differentiated in vitro into cells expressing markers of ectoderm, mesoderm, and endoderm, and when injected into immunodeficient mice, gave raise to tumors containing differentiated derivatives of the 3 germ layers. Finally, we also reprogrammed fibroblasts from a 2-year-old horse. The reprogrammed cells were similar to iPSCs derived from neonatal fibroblasts in terms of morphology, expression of pluripotency markers, and differentiation ability. The generation of these novel cell lines constitutes an important step toward the understanding of pluripotency in the horse, and paves the way for iPSC technology to potentially become a powerful research and clinical tool in veterinary biomedicine.

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“…However, the used technology still needs optimization as they undergo spontaneous differentiation over long-term culture, irrespective of the source of derivation, that is, in vivo or in vitro produced blastocysts. Canine ESCs have also been isolated from pre-implantation stage embryos with both in vitro and in vivo differentiation potential (Hatoya et al 2006;Hayes et al 2008;Schneider et al 2008;Vaags et al 2009;Wilcox et al 2009;Schneider et al 2010). These canine ESCs were able to undergo self-renewal for prolonged periods of culture while remaining undifferentiated, and expressed a characteristic pluripotency gene expression profile, such as OCT4, SSEA3 and SOX2.…”

Section: Embryonic Stem Cells In Domestic Animalsmentioning

confidence: 99%

Use of pluripotent stem cells for reproductive medicine: are we there yet?

Duggal

Heindryckx

Deroo

et al. 2014

Veterinary Quarterly

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In recent years, pluripotent stem cells have demonstrated to be exciting tools to understand embryonic development, cell lineage specification, tissue generation and repair, and various other biological processes. In addition, the identification and isolation of germ line stem cells has given more insight into germ cell biology at the molecular level and into the underlying causes of infertility which was not possible earlier. The recent derivation of in vitro derived sperm and oocytes from pluripotent stem cells in the mouse model represents a major breakthrough in the field and substantiates the critical relevance of stem cells as a potential alternative resource for treating infertility. Although the past years have yielded compelling information in understanding germ cell development via in vitro stem cell assays, extended investigative research is necessary in order to derive fully functional 'artificial gametes' in a safe way for future therapeutic applications.

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Derivation and Characterization of Canine Embryonic Stem Cell Lines with In Vitro and In Vivo Differentiation Potential

Abstract: ABSTRACT

Cited by 73 publications

References 81 publications

Generation of Leukemia Inhibitory Factor and Basic Fibroblast Growth Factor-Dependent Induced Pluripotent Stem Cells from Canine Adult Somatic Cells

Generation of Leukemia Inhibitory Factor and Basic Fibroblast Growth Factor-Dependent Induced Pluripotent Stem Cells from Canine Adult Somatic Cells

Derivation and Characterization of Induced Pluripotent Stem Cells from Equine Fibroblasts

Use of pluripotent stem cells for reproductive medicine: are we there yet?

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