Derivation and preliminary characterization of pluripotent cell lines from porcine and bovine blastocysts

Evans, Martin; Notarianni, Elena; Laurie, S.; Moor, R. M.

doi:10.1016/0093-691x(90)90603-q

Cited by 139 publications

(88 citation statements)

References 2 publications

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“…Notarianni et al [4,5] and Piedrahita et al [6,7] attempted isolation from both sheep and pig embryos. Cell lines from porcine blastocysts were obtained by Strojek et al [8], Evans et al [9] and Talbot et al [10] , and from bovine blastocysts by Saito et al [11]. None of these studies proved that the cell lines isolated were totipotent, although these cells showed some differentiation in vitro.…”

Section: Discussionmentioning

confidence: 99%

Establishment of Embryonic Stem (ES) Cell-Like Cell Lines Derived from Bovine Blastocysts Obtained by In Vitro Culture of Oocytes Matured and Fertilized In Vitro.

Hamano¹,

Watanabe²,

Azuma

et al. 1998

Journal of Reproduction and Development

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Section: Discussionmentioning

confidence: 99%

Establishment of Embryonic Stem (ES) Cell-Like Cell Lines Derived from Bovine Blastocysts Obtained by In Vitro Culture of Oocytes Matured and Fertilized In Vitro.

Hamano¹,

Watanabe²,

Azuma

et al. 1998

Journal of Reproduction and Development

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“…Intact porcine blastocysts were used by Evans et al [14], Piedrahita et al [6], Hocherau de Reviers and Perreau [22], Gerfen and Wheeler [27], Miyoshi et al [36], Li et al [19] and Kim et al [39]. On the other hand, Talbot et al [47] does not recommend culture of intact pig blastocysts because this approach results in attachment and growth of the trophoblast and primitive endoderm, whilst the embryoblast typically does not grow and instead degenerates or differentiates.…”

Section: Icm Isolationmentioning

confidence: 99%

“…In the mouse, implantation is an invasive and rapid process. On the Accepted for publication: September 13, 2007 Correspondence: I. Vackova (e-mail: ivackova@seznam.cz, vackova@vuzv.cz) other hand, in most ungulates, including pigs, implantation occurs only after a considerable delay during which the trophectoderm proliferates rapidly and the inner cell mass (ICM) forms a quiescent embryonic disc [14].…”

mentioning

confidence: 99%

Putative Embryonic Stem Cell Lines from Pig Embryos

Vacková

Ungrova

Lopes

2007

Journal of Reproduction and Development

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Abstract. Embryonic stem cells (ES cells)were first established in the mouse, and they represent a population of pluripotent, undifferentiated cells derived from early embryos that is capable of proliferating without any limitation in an undifferentiated state. These cells retain the ability to differentiate in vitro or in vivo into derivates of all three germ layers, and when injected into blastocysts, they can participate in the formation of all tissues, including gonads (germ-line chimeras). It is possible to transfect them with a gene of interest, and the resulting transgenic cell lines can also be used for production of chimeras. Unfortunately, mammalian germ-line chimeras that can carry an inserted gene into their progeny have only been produced in the mouse. Logically, before application of stem cell therapies into a human medicine, it is necessary to verify the efficiency and safety of these methods with an acceptable animal model. The pig is currently used as a very convenient animal for pre-clinical applications, and therefore establishment of porcine ES cell lines is highly needed; unfortunately, no convincing ES cell lines have been produced in this species (and other domestic animals) to date. In this article, we discuss the recent advances in this field, especially oriented on possible reasons and obstacles why derivation of porcine ES cell lines is still unsuccessful.

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“…There have been many attempts to gain ES cell lines in such mammalian species as hamster [10], pig [11][12][13], cow [11,14], mink [15], sheep [12], rabbit [16], rat [17], monkey [18] and human [19]. However, no ES-like cells mentioned distinctly contributed chimeras.…”

mentioning

confidence: 99%

Effects of Feeder Cells on the Establishment of ES Cells from Mice and Rats. The Difference between Allogenetic and Xenogenetic Feeders.

Kawase

Yoshimizu

Hashimoto

2000

Journal of Reproduction and Development

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Abstract. The technology of mouse embryonic stem (ES) cells is clearly proving to be a very successful method for producing transgenic mice. Although there has been considerable efforts to establish ES cell lines from other animals, most of all lines obtained, so far, have been derived from mice. Here, we examined effects of allogenetic and xenogenetic feeders on the establishment of ES cells from mice and rats. We first used three types of feeder cells on the establishment of rat ES cells; mouse primary embryonic fibroblsts (M-PEF), rat primary embryonic fibroblasts (R-PEF) and a mouse embryonic fibroblast cell line, SL10. We could gain some rat ES cell lines only on R-PEF. RES-DA1 cell line from DA rat strain, has the same morphology as mouse ES cells, alkaline phosphatase activity and the 4C9 antigen. We, moreover, examined effects of M-PEF and R-PEF feeders on the establishment of mouse ES cells in C57BL/6 mice. The mouse ES cell line could be established only on M-PEF feeders. Both rat and mouse ES cell lines had pluripotenciality. These results suggested that the establishment of ES cell lines is more effective on allogenetic feeder cells than on xenogenetic cells.

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Derivation and preliminary characterization of pluripotent cell lines from porcine and bovine blastocysts

Cited by 139 publications

References 2 publications

Establishment of Embryonic Stem (ES) Cell-Like Cell Lines Derived from Bovine Blastocysts Obtained by In Vitro Culture of Oocytes Matured and Fertilized In Vitro.

Establishment of Embryonic Stem (ES) Cell-Like Cell Lines Derived from Bovine Blastocysts Obtained by In Vitro Culture of Oocytes Matured and Fertilized In Vitro.

Putative Embryonic Stem Cell Lines from Pig Embryos

Effects of Feeder Cells on the Establishment of ES Cells from Mice and Rats. The Difference between Allogenetic and Xenogenetic Feeders.

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