Derivation of germ-line-competent embryonic stem cell lines from preblastocyst mouse embryos

Tesar, Paul J.

doi:10.1073/pnas.0503231102

Cited by 92 publications

(73 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several reports have demonstrated the importance of the genetic background of mice on early embryonic development (Bagis et al 2001) and the establishment of ES cells (Kawase et al 1994). Most of the ES cell lines, including the single BTM-derived ES cells described by Wakayama et al, were derived from the 129/SV strain (Sukoyan et al 2002, Tesar 2005, Wakayama et al 2007, which has been shown to be the best mouse strain for establishing an ES cell line.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Development of single mouse blastomeres into blastocysts, outgrowths and the establishment of embryonic stem cells

Lorthongpanich¹,

Yang²,

Piotrowska-Nitsche³

et al. 2008

Reproduction

View full text Add to dashboard Cite

The recently developed technique of establishing embryonic stem (ES) cell lines from single blastomeres (BTMs) of early mouse and human embryos has created significant interest in this source of ES cells. However, sister BTMs of an early embryo might not have equal competence for the development of different lineages or the derivation of ES cells. Therefore, single BTMs from two-and four-cell embryos of outbred mice were individually placed in sequential cultures to enhance the formation of the inner cell mass (ICM) and the establishment of embryonic outgrowth. The outgrowths were then used for the derivation of ES cell lines. Based on the expression of ICM (Sox2 ) and trophectoderm (Cdx2 ) markers, it was determined that ICM marker was lacking in blastocysts derived from 12% of BTMs from two-cell stage and 20% from four-cell stage. Four ES cell lines (5.6%; 4/72) were established ater culture of single BTMs from twocell embryos, and their pluripotency was demonstrated by their differentiation into neuronal cell types. Our results demonstrate that sister BTMs of an early embryo are not equally competent for ICM marker expression. However, we demonstrated the feasibility of establishing ES cells from a single BTM of outbred mice. Reproduction (2008) 135 805-813

show abstract

Section: Discussionmentioning

confidence: 99%

“…Embryonic stem (ES) cells have been successfully established from early embryos rather than using the ICM of a blastocyst (Eistetter 1989, Strelchenko et al 2004, Tesar 2005. It is noted that only a small number of BTMs from 8-cell (Delhaise et al 1996) and 16-cell (Eistetter 1989) mouse embryos were viable for deriving ES cells.…”

Section: Introductionmentioning

confidence: 99%

Development of single mouse blastomeres into blastocysts, outgrowths and the establishment of embryonic stem cells

Lorthongpanich¹,

Yang²,

Piotrowska-Nitsche³

et al. 2008

Reproduction

View full text Add to dashboard Cite

show abstract

“…This technique requires specialist expertise, however, and has not been widely adopted. Successful derivations have also been reported from morulae and even from isolated eight-cellstage blastomeres, leading to the suggestion that ESCs might represent a very early stage of development (Eistetter 1992;Tesar 2005). In these studies, however, cultures are invariably left intact for several days.…”

Section: Derivation Of Escsmentioning

confidence: 99%

Pluripotency in the Embryo and in Culture

Nichols

Smith

2012

Cold Spring Harbor Perspectives in Biology

272

254

View full text Add to dashboard Cite

SUMMARYSpecific cells within the early mammalian embryo have the capacity to generate all somatic lineages plus the germline. This property of pluripotency is confined to the epiblast, a transient tissue that persists for only a few days. In vitro, however, pluripotency can be maintained indefinitely through derivation of stem cell lines. Pluripotent stem cells established from the newly formed epiblast are known as embryonic stem cells (ESCs), whereas those generated from later stages are called postimplantation epiblast stem cells (EpiSCs). These different classes of pluripotent stem cell have distinct culture requirements and gene expression programs, likely reflecting the dynamic development of the epiblast in the embryo. In this chapter we review current understanding of how the epiblast forms and relate this to the properties of derivative stem cells. We discuss whether ESCs and EpiSCs are true counterparts of different phases of epiblast development or are culture-generated phenomena. We also consider the proposition that early epiblast cells and ESCs may represent a naïve ground state without any prespecification of lineage choice, whereas later epiblasts and EpiSCs may be primed in favor of particular fates.

show abstract

“…Tesar showed that whole denuded (zona-free) embryos from early preimplantation stages (zygote, four-, eight-cell, and morula stage) could develop, attach, and spread out to form colony-like structures in culture, after they reached a morula-like stage (preblastocyst) on feeder layers without cavitation. Tesar demonstrated that putative ES cell lines could be obtained in this way with high efficiency from 129S2/SvHsd embryos using standard culture medium (Knockout-DMEM, KDMEM) for derivation and maintenance, which are typically used for ES cell derivation (Tesar, 2005). It is currently not known whether this method can be successfully applied to nontraditional mouse strains, nor is it well understood what is different between preblastocysts and zona-intact embryos (blastocysts), in terms of development and characterization of ES cells derived from these two different developmental stages.…”

Section: Introductionmentioning

confidence: 99%

“…In general, the early preimplantation embryos prior to blastocyst development have been evaluated by dissociating them into individual blastomeres for ES cell derivation, but this method produces ES cell lines at very low efficiencies (Chung et al, 2006;Delhaise et al, 1996;Wakayama et al, 2007). Recently, a new technique involving removal of the zona pellucida has been introduced, with subsequent blocking of cavitation in the embryo (Tesar, 2005). Tesar showed that whole denuded (zona-free) embryos from early preimplantation stages (zygote, four-, eight-cell, and morula stage) could develop, attach, and spread out to form colony-like structures in culture, after they reached a morula-like stage (preblastocyst) on feeder layers without cavitation.…”

Section: Introductionmentioning

confidence: 99%

Established Preblastocyst- and Blastocyst-Derived ES Cell Lines Have Highly Similar Gene Expression Profiles, Despite Their Differing Requirements for Derivation Culture Conditions

Kim

Park

Amano

et al. 2012

Cellular Reprogramming

View full text Add to dashboard Cite

The efficiency of embryonic stem (ES) cell derivation relies on an optimized culture medium and techniques for treating preimplantation stage embryos. Recently, ES cell derivation from the preblastocyst developmental stage was reported by removing the zona pellucida from embryos of the most efficient strain for ES cell derivation (129Sv) during early preimplantation. Here, we showed that ES cells can be efficiently derived and maintained in a modified medium (MEMa), from preblastocysts of a low-efficiency mouse strain (a hybrid consisting of 50% B6, 25% CBA, and 25% DBA). Preblastocyst-derived ES cell lines were normal in terms of pluripotency-related protein expression, and chromosome number. Also, preblastocyst-derived ES cell lines from various culture conditions showed pluripotency in vivo through teratoma analysis. Interestingly, ES cell lines produced from preblastocysts and blastocysts, regardless of the derivation culture conditions, are nearly indistinguishable by their global gene expression profiles.

show abstract

Derivation of germ-line-competent embryonic stem cell lines from preblastocyst mouse embryos

Cited by 92 publications

References 31 publications

Development of single mouse blastomeres into blastocysts, outgrowths and the establishment of embryonic stem cells

Development of single mouse blastomeres into blastocysts, outgrowths and the establishment of embryonic stem cells

Pluripotency in the Embryo and in Culture

Established Preblastocyst- and Blastocyst-Derived ES Cell Lines Have Highly Similar Gene Expression Profiles, Despite Their Differing Requirements for Derivation Culture Conditions

Contact Info

Product

Resources

About