Derivation of Oocytes from Mouse Embryonic Stem Cells

Hübner, Karin; Fuhrmann, Gregor; Christenson, Lane K.; Kehler, James; Reinbold, Rolland; Fuente, Rabindranath De La; Wood, Jennifer R.; Strauss, Jerome F.; Boiani, Michele; Schöler, Hans R.

doi:10.1126/science.1083452

Cited by 970 publications

(794 citation statements)

References 38 publications

Supporting

Mentioning

716

Contrasting

Unclassified

Order By: Relevance

“…Embryonic stem (ES) cells are defined by the properties of pluripotency and self-renewal, and are capable of differentiating into cell lineages from all three germ layers as well as into germ cells [1][2][3][4][5]. They are derived from the inner cell mass of blastocysts and provide a powerful model system to study the molecular mechanisms regulating early and late lineage specific differentiation.…”

Section: Introductionmentioning

confidence: 99%

Differentiation of murine embryonic stem cells induces progesterone receptor gene expression

Sauter

McDermid

Weinberg

et al. 2005

Experimental Cell Research

View full text Add to dashboard Cite

The role of steroid hormone receptors in very early embryonic development remains unknown. Clearly, expression during organogenesis is important for tissue-specific development. However, progesterone receptor (PR) and estrogen receptors (ERα, ERβ), are expressed during early development through the blastocyst stage in mice and other species, and yet are not essential for embryonic viability. We have utilized the mouse embryonic stem (mES) cell model to investigate the regulated expression of these receptors during differentiation. Surprisingly, one of the earliest changes in gene expression in response to a differentiation signal observed is PR gene induction. It parallels the time course of expression for the patterning genes Hoxb1 and Hoxa5. Unexpectedly, PR gene expression is not regulated in an estrogen dependent manner by endogenous ERs or by transiently overexpressed ERα. Our results suggest a potentially novel mechanism of PR gene regulation within mES cells compared to adult tissues and the possibility of unique targets of PR action during early mES cell differentiation

show abstract

Section: Introductionmentioning

confidence: 99%

Differentiation of murine embryonic stem cells induces progesterone receptor gene expression

Sauter

McDermid

Weinberg

et al. 2005

Experimental Cell Research

View full text Add to dashboard Cite

show abstract

“…The efficiency of differentiation from ES or iPS cells into haploid male germ cells is generally reported to be low. [14][15][16][17][18][19][20][21][22][23][24] Although there is no evidence for the speculation that this efficiency might be higher using AFS cells, to our opinion, the here reviewed knowledge warrants testing this new stem cell type for their usability for the in vitro investigation of sperm production (Figure 1).…”

Section: Amniotic Fluid Stem (Afs) Cells Could Be a Useful Tool To Stmentioning

confidence: 89%

“…Since spontaneous induction of meiosis is low, overexpression of genes of the DAZ gene family can be performed to induce meiosis for haploid spermatids 5,27 ( Figure 1). Finally, in mice it is possible to test the biological function of so-obtained haploid male gametes either by transplantation into busulfan-mediated sperm-depleted testes to check in vivo differentiation, or by intracytoplasmic injection into recipient oocytes to test whether this supports cleavage [14][15][16] (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Amniotic fluid stem cell-based models to study the effects of gene mutations and toxicants on male germ cell formation

Gundacker¹,

Dolznig²,

Mikula³

et al. 2012

Asian J Androl

View full text Add to dashboard Cite

Male infertility is a major public health issue predominantly caused by defects in germ cell development. In the past, studies on the genetic regulation of spermatogenesis as well as on negative environmental impacts have been hampered by the fact that human germ cell development is intractable to direct analysis in vivo. Compared with model organisms including mice, there are fundamental differences in the molecular processes of human germ cell development. Therefore, an in vitro model mimicking human sperm formation would be an extremely valuable research tool. In the recent past, both human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells have been reported to harbour the potential to differentiate into primordial germ cells and gametes. We here discuss the possibility to use human amniotic fluid stem (AFS) cells as a biological model. Since their discovery in 2003, AFS cells have been characterized to differentiate into cells of all three germ layers, to be genomically stable, to have a high proliferative potential and to be non-tumourigenic. In addition, AFS cells are not subject of ethical concerns. In contrast to iPS cells, AFSs cells do not need ectopic induction of pluripotency, which is often associated with only imperfectly cleared epigenetic memory of the source cells. Since AFS cells can be derived from amniocentesis with disease-causing mutations and can be transfected with high efficiency, they could be used in probing gene functions for spermatogenesis and in screening for male reproductive toxicity.

show abstract

“…[5, 7,13,16,19,22,24,25,27] Cellules Sertoli, cellules Leydig, cellules de la granulosa, cellules de la thèque…”

Section: V-kit Hardy-zuckerman 4 Feline Sarcoma Viral Oncogene Homologunclassified

Productionin vitrode cellules germinales murines et humaines à partir de cellules souches pluripotentes

et al. 2011

View full text Add to dashboard Cite

Derivation of Oocytes from Mouse Embryonic Stem Cells

Cited by 970 publications

References 38 publications

Differentiation of murine embryonic stem cells induces progesterone receptor gene expression

Differentiation of murine embryonic stem cells induces progesterone receptor gene expression

Amniotic fluid stem cell-based models to study the effects of gene mutations and toxicants on male germ cell formation

Productionin vitrode cellules germinales murines et humaines à partir de cellules souches pluripotentes

Contact Info

Product

Resources

About