Derivation of the complete msp4 gene sequence of Anaplasma marginale without cloning

Oberle, S M; Barbet, Anthony F.

doi:10.1016/0378-1119(93)90482-i

Cited by 31 publications

(26 citation statements)

References 12 publications

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“…The msp4 gene is known to be difficult to clone in E. coli (46), an observation that may be clarified by the genome sequence. Msp4 is flanked by two msp3 pseudogenes: one 336 bp upstream and the other 4,687 bp downstream from msp4.…”

Section: Resultsmentioning

confidence: 99%

Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins

Brayton¹,

Kappmeyer²,

Herndon³

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

248

301

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The rickettsia Anaplasma marginale is the most prevalent tick-borne livestock pathogen worldwide and is a severe constraint to animal health. A. marginale establishes lifelong persistence in infected ruminants and these animals serve as a reservoir for ticks to acquire and transmit the pathogen. Within the mammalian host, A. marginale generates antigenic variants by changing a surface coat composed of numerous proteins. By sequencing and annotating the complete 1,197,687-bp genome of the St. Maries strain of A. marginale, we show that this surface coat is dominated by two families containing immunodominant proteins: the msp2 superfamily and the msp1 superfamily. Of the 949 annotated coding sequences, just 62 are predicted to be outer membrane proteins, and of these, 49 belong to one of these two superfamilies. The genome contains unusual functional pseudogenes that belong to the msp2 superfamily and play an integral role in surface coat antigenic variation, and are thus distinctly different from pseudogenes described as byproducts of reductive evolution in other Rickettsiales.rickettsiales ͉ bacterial artificial chromosome ͉ St. Maries strain

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Section: Resultsmentioning

confidence: 99%

Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins

Brayton¹,

Kappmeyer²,

Herndon³

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

248

301

View full text Add to dashboard Cite

show abstract

“…MSP4 and MSP5 are encoded by single-copy genes. Although MSP4 is highly conserved (52,53,54,112), information about its function is not available. MSP5 is also a highly conserved surface protein that has been proven effective as a diagnostic antigen and used in a competitive enzymelinked immunosorbent assay (ELISA) commercially available in the United States (163).…”

Section: A Marginale Genome and Major Surface Protein Genesmentioning

confidence: 99%

Antigens and Alternatives for Control ofAnaplasma marginaleInfection in Cattle

et al. 2003

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Anaplasmosis, a tick-borne cattle disease caused by the rickettsia Anaplasma marginale, is endemic in tropical and subtropical areas of the world. The disease causes considerable economic loss to both the dairy and beef industries worldwide. Analyses of 16S rRNA, groESL, and surface proteins have resulted in the recent reclassification of the order Rickettsiales. The genus Anaplasma, of which A. marginale is the type species, now also includes A. bovis, A. platys, and A. phagocytophilum, which were previously known as Ehrlichia bovis, E. platys, and the E. phagocytophila group (which causes human granulocytic ehrlichiosis), respectively. Live and killed vaccines have been used for control of anaplasmosis, and both types of vaccines have advantages and disadvantages. These vaccines have been effective in preventing clinical anaplasmosis in cattle but have not blocked A. marginale infection. Thus, persistently infected cattle serve as a reservoir of infective blood for both mechanical transmission and infection of ticks. Advances in biochemical, immunologic, and molecular technologies during the last decade have been applied to research of A. marginale and related organisms. The recent development of a cell culture system for A. marginale provides a potential source of antigen for the development of improved killed and live vaccines, and the availability of cell culture-derived antigen would eliminate the use of cattle in vaccine production. Increased knowledge of A. marginale antigen repertoires and an improved understanding of bovine cellular and humoral immune responses to A. marginale, combined with the new technologies, should contribute to the development of more effective vaccines for control and prevention of anaplasmosis

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“…In the outer membrane of A. marginale, six major surface proteins (MSP) were initially characterized: MSP1a, MSP1b, MSP2, MSP3, MSP4, and MSP5 (Palmer & McGuire 1984, Barbet et al 1987, Oberle et al 1988, Tebele et al 1991, Oberle & Barbet 1993. However, none of these recombinant proteins, individually or collectively, recreated the immunoprotective capacity of the outer membrane (Brayton et al 2006).…”

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confidence: 99%

IgG and IgG2 antibodies from cattle naturally infected with Anaplasma marginale recognize the recombinant vaccine candidate antigens VirB9, VirB10, and elongation factor-Tu

Araújo

Costa

Ramos

et al. 2008

Mem. Inst. Oswaldo Cruz

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Derivation of the complete msp4 gene sequence of Anaplasma marginale without cloning

Cited by 31 publications

References 12 publications

Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins

Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins

Antigens and Alternatives for Control ofAnaplasma marginaleInfection in Cattle

IgG and IgG2 antibodies from cattle naturally infected with Anaplasma marginale recognize the recombinant vaccine candidate antigens VirB9, VirB10, and elongation factor-Tu

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