Derivation of the formula for the filtration coefficient by application of Poiseuille's law in membrane transport

Jarzyńska, Maria; Pietruszka, Mariusz

doi:10.5586/asbp.2009.012

Cited by 3 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The next step is to establish an equation that can describe the effect of flow on the passive permeation of R123. For this purpose, Equation ( 9) can be re-written in this form [41]:…”

Section: Mbarmentioning

confidence: 99%

Microphysiological Conditions Do Not Affect MDR1-Mediated Transport of Rhodamine 123 above an Artificial Proximal Tubule

Namazian Jam,

Gottlöber,

Hempel

et al. 2023

Biomedicines

View full text Add to dashboard Cite

Despite disadvantages, such as high cost and their poor predictive value, animal experiments are still the state of the art for pharmaceutical substance testing. One reason for this problem is the inability of standard cell culture methods to emulate the physiological environment necessary to recapitulate in vivo processes. Microphysiological systems offer the opportunity to close this gap. In this study, we utilize a previously employed microphysiological system to examine the impact of pressure and flow on the transportation of substances mediated by multidrug resistance protein 1 (MDR1) across an artificial cell-based tubular barrier. By using a miniaturized fluorescence measurement device, we could continuously track the MDR1-mediated transport of rhodamine 123 above the artificial barrier over 48 h. We proved that applying pressure and flow affects both active and passive transport of rhodamine 123. Using experimental results and curve fittings, the kinetics of MDR1-mediated transport as well as passive transport were investigated; thus, a kinetic model that explains this transport above an artificial tubular barrier was identified. This kinetic model demonstrates that the simple Michaelis–Menten model is not an appropriate model to explain the MDR1-mediated transport; instead, Hill kinetics, with Hill slope of n = 2, is a better fit. The kinetic values, Km, Vmax, and apparent permeability (Papp), obtained in this study are comparable with other in vivo and in vitro studies. Finally, the presented proximal tubule-on-a-chip can be used for pharmaceutical substance testing and to investigate pharmacokinetics of the renal transporter MDR1.

show abstract

“…The next step is to establish an equation that can describe the effect of flow on the passive permeation of R123. For this purpose, Equation ( 9) can be re-written in this form [41]:…”

Section: Mbarmentioning

confidence: 99%

Microphysiological Conditions Do Not Affect MDR1-Mediated Transport of Rhodamine 123 above an Artificial Proximal Tubule

Namazian Jam,

Gottlöber,

Hempel

et al. 2023

Biomedicines

View full text Add to dashboard Cite

show abstract

“…In order to describe the membrane transport for binary nonelectrolyte solutions, the transport equations derived by Katchalsky and Kedem [3][4][5][6] in 1985 turned out to be highly useful. The Kedem-Katchalsky (K-K) transport equations played an important role in the literature of permeation through both biological and artificial membranes.…”

Section: Introductionmentioning

confidence: 99%

Diffusion Characteristics in Ethyl Alcohol and Glucose Solutions Using Kedem‐Katchalsky Equations

Jarzyńska¹,

Staryga

Kluza

et al. 2019

Chem Eng & Technol

Self Cite

View full text Add to dashboard Cite

Formulas for calculating the viscosity and diffusion coefficients for two-component aqueous non-electrolyte solutions as a function of the solution concentration under isothermal conditions were derived. The dependence of diffusion coefficient, filtration coefficient, and dynamic viscosity coefficient for these solutions on the solution concentration was estimated and evaluated. Based on the obtained formulas, the results of diffusion coefficient, membrane filtration coefficient, and dynamic viscosity calculations for aqueous solutions of ethanol and glucose are presented and compared with literature data.

show abstract

The application of the Kedem–Katchalsky equations to membrane transport of ethyl alcohol and glucose

Jarzyńska

Pietruszka

2011

Desalination

View full text Add to dashboard Cite

Derivation of the formula for the filtration coefficient by application of Poiseuille's law in membrane transport

Cited by 3 publications

References 7 publications

Microphysiological Conditions Do Not Affect MDR1-Mediated Transport of Rhodamine 123 above an Artificial Proximal Tubule

Microphysiological Conditions Do Not Affect MDR1-Mediated Transport of Rhodamine 123 above an Artificial Proximal Tubule

Diffusion Characteristics in Ethyl Alcohol and Glucose Solutions Using Kedem‐Katchalsky Equations

The application of the Kedem–Katchalsky equations to membrane transport of ethyl alcohol and glucose

Contact Info

Product

Resources

About