Derivatives of Cucurbitacin-E-glucoside produced by Curvularia lunata NRRL 2178: Anti-inflammatory, antipyretic, antitumor activities, and effect on biochemical parameters

Abdelkhalek, Abdelfattah A.; Sharaf, A.; Rabie, Mohamed A.; El‐Subbagh, Hussein I.

doi:10.1016/j.fjps.2017.04.006

Cited by 12 publications

(4 citation statements)

References 24 publications

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“…Full assignment of all H and 13 C resonances as well as correlations observed in COSY, HSQC, ROESY and HMBC experiments are provided in Supplementary Table 10, and they are in agreement with 1 H and 13 C NMR data of cucurbitacins and their glycosides as reported in literature (Ayyad, et al 2012;Chawech, et al 2015;Abdelkhalek, et al 2017). Compound 5: Cucurbitacin I mg of a reference sample of cucurbitacin I bought from Sigma-Aldrich was dissolved in 650 μl methanol-d 4 , and 1D 1 H NMR as swell as 2D COSY, HSQC, and HMBC spectra were acquired.…”

Section: A B C Dsupporting

confidence: 83%

An Independent Evolutionary Origin for Insect Deterrent Cucurbitacins inIberis amara

Dong

Almeida

Pollier

et al. 2021

Molecular Biology and Evolution

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Pieris rapae and Phyllotreta nemorum are Brassicaceae specialists, but do not feed on Iberis amara spp. that contain cucurbitacins. The cucurbitacins are highly oxygenated triterpenoid, occurring widespread in cucurbitaceous species and in a few other plant families. Using de-novo assembled transcriptomics from I. amara, gene co-expression analysis and comparative genomics, we unraveled the evolutionary origin of the insect deterrent cucurbitacins in I. amara. Phylogenetic analysis of five oxidosqualene cyclases and heterologous expression allowed us to identify the first committed enzyme in cucurbitacin biosynthesis in I. amara, cucurbitadienol synthase (IaCPQ). In addition, two species-specific cytochrome P450s (CYP708A16 and CYP708A15) were identified that catalyse the unique C16 and C22 hydroxylation of the cucurbitadienol backbone, enzymatic steps that have not been reported before. Furthermore, the draft genome assembly of I. amara showed that the IaCPQ was localized to the same scaffold together with CYP708A15 but spanning over 100 kb, this contrasts with the highly organized cucurbitacin gene cluster in the cucurbits. These results reveal that cucurbitacin biosynthesis has evolved convergently via different biosynthetic routes in different families rather than through divergence from an ancestral pathway. This study thus provides new insight into the mechanism of recurrent evolution and diversification of a plant defensive chemical.

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Section: A B C Dsupporting

confidence: 83%

An Independent Evolutionary Origin for Insect Deterrent Cucurbitacins inIberis amara

Dong

Almeida

Pollier

et al. 2021

Molecular Biology and Evolution

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“…In developing countries, around 70-80% of the population relies on medicinal herbs and plants as primary health care [50]. Although modern drugs are accessible and effective in curing many diseases, herbal remedies are gaining popularity day by day owing to its fewer side effects and toxicity [51]. In our present study, we evaluated the medicinal plant extract collected from the Western region of Nepal for their potential health benefits such as antioxidant, anti-cancer, and anti-inflammatory activities.…”

Section: Discussionmentioning

confidence: 99%

In-vitro antioxidant, anti-cancer, and anti-inflammatory activities of selected medicinal plants from western Nepal

et al. 2020

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Background This study was focused on the measurement of anticancer properties of six medicinal plants from western Nepal in three cell lines; HeLa, Hep3B, and HCT116, and anti-inflammatory properties in RAW 264.7 cell line through NO, PGE2, and TNF-α production. In addition, the phytochemical screening, total phenolic, flavonoid content, and antioxidant properties were evaluated. Results The qualitative phytochemical analysis revealed the presence of different secondary metabolite and range of total phenolic and total flavonoid content. The highest antioxidant activities were observed in Bergenia pacumbis against both DPPH (IC50 = 25.97 ± 0.19 μg/mL) and ABTS (IC50 = 14.49 ± 0.40 μg/mL). Furthermore, the highest antiproliferative effect against cervical, liver, and colon cancer cell lines were observed in Melia azedarach as IC50 values of 10.50, 5.30, and 1.57 μg/mL respectively, while the strongest anti-metastatic potential on liver cancer cell line was found in Pleurospermum benthamii. In addition, P. benthamii showed the most potent anti-inflammatory effect in RAW264.7 murine macrophage cells. Conclusion This study provided the evidence for M. azedarach and P. benthamii to have great anticancer potential and finding builds the enough scientific backgrounds in future to isolate and purify the bioactive compounds for further applications.

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“…28,29 Although modern medicines are very effective in treating various diseases, traditional and natural remedies have attracted much attention because of their better toxicity profile and fewer adverse effects. 30 This study used GC-MS to screen PLE for possible health-promoting effects, such as antioxidant and anticancer properties. The DPPH free radical assay is commonly used to determine free radical scavenging abilities.…”

Section: Discussionmentioning

confidence: 99%

Partially Purified Polysaccharides from Lentinus edodes (Mushroom) Scavenge Free Radicals and Induce Apoptosis in MCF-7 Cancer Cells by Regulating Apoptotic Genes

Ponnusamy,

Uddandrao,

Sasikumar

et al. 2024

Ind. J. Pharm. Edu. Res

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Background: Therapeutic products obtained from nature have been gaining attention for health improvement, disease treatment, and drug development. Traditional therapeutic compounds from plants, animals, and microorganisms and their bioactive constituents have led to the development of therapeutics for various diseases, including cancer. Mushrooms are widely consumed products because of their nutritional and therapeutic potential. Materials and Methods: This study aimed to extract partially purified polysaccharides from Lentinus edodes (PLE) to a study to identify bioactive compounds by GC-MS and HPLC. Furthermore, we examined PLE to identify its antioxidant potential and effect on breast cancer cells (MCF-7 cell lines). Results: GC-MS revealed the presence of 45 bioactive components from PLE, and HPLC analysis showed the presence of β-glucan. Furthermore, PLE showed potential antioxidant activity, which was confirmed by significant free radical (DPPH, FRAP, and ABTS) scavenging and total antioxidant activity analysis. In addition, PLE showed noteworthy anticancer activity, substantiated by decreased cell viability in a dose-dependent manner (100, 200, 300, and 400µg/ mL) manner. On the other hand, RT-PCR analysis revealed that PLE potentially up-regulated caspase-3, toll-like receptor 4, and Bcl-2-associated X protein and concurrently down-regulated B-cell lymphoma 2 (Bcl-2), which confirmed that PLE can induce apoptosis in MCF-7 cancer cells. Conclusion: Hence, this study revealed that PLE can induce apoptosis in cancer cells and fight against breast cancer through its antioxidant potential. These results recommend that PLE might be considered a nutraceutical for managing breast cancer.

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Derivatives of Cucurbitacin-E-glucoside produced by Curvularia lunata NRRL 2178: Anti-inflammatory, antipyretic, antitumor activities, and effect on biochemical parameters

Cited by 12 publications

References 24 publications

An Independent Evolutionary Origin for Insect Deterrent Cucurbitacins inIberis amara

An Independent Evolutionary Origin for Insect Deterrent Cucurbitacins inIberis amara

In-vitro antioxidant, anti-cancer, and anti-inflammatory activities of selected medicinal plants from western Nepal

Partially Purified Polysaccharides from Lentinus edodes (Mushroom) Scavenge Free Radicals and Induce Apoptosis in MCF-7 Cancer Cells by Regulating Apoptotic Genes

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