Derivatization-based magnetic dummy molecularly imprinted polymers integrated with 4-plex stable isotope labeling derivatization strategy for specific and rapid determination of L-hydroxyproline in human serum

Application progress of magnetic molecularly imprinted polymers chemical sensors in the detection of biomarkers

“…Currently, for biomarkers with high research value, researchers choose substitutes with similar molecular structure as the imprinted templates to solve the problems of template molecules, such as high price, difficulty in obtaining materials, or ease of infection, degradation and leakage. Zhu et al 13 developed an integrated strategy based on 4-plex stable isotope labeling derivatization combined with dummy magnetic molecularly imprinted polymers. In this strategy, the structural analogue derivative of Hyp with 6- N -ethyl-rhodamine 6G- N -hydroxysuccinimidyl formate was synthesized and used as a novel dummy template to prepare dummy-MMIPs.…”

“…However, MIPs prepared by traditional methods have some limitations, such as low efficiency of the sample pretreatment, few effective imprinting cavities and low molecular weight of the template. In order to solve the above problems, researchers not only improved the imprinting method, 12–14 but also combined it with graphene oxide, 15 carbon dots (CDs), 16 gold nanoparticles (Au NPs), 17 carbon nanotubes (CNT), 18 and magnetic nanoparticles (MNPs). 19…”

“…In another example, Fang et al 28 first proposed the magnetic metal oxide affinity chromatography (MOAC) MIT for the separation and analysis of lysozyme in human serum and urine samples. Xian-En Zhao and others 13,29–31 designed a series of strategies combining multiplex tags with chemical isotope labeling and magnetic dispersive solid phase extraction for ultra-high performance liquid chromatography tandem mass spectrometry selective extraction and high-throughput determination of serum or various biomarkers in urine samples. Therefore, other types of sensors will not be described in detail below.…”

Preparative isolation of 1,1‐diphenyl‐2‐picrylhydrazyl inhibitors from Ribes himalense using medium‐pressure and two‐dimensional reversed‐phase/reversed‐phase liquid chromatography guided by an online HPLC‐1, 1‐diphenyl‐2‐picrylhydrazyl assay

“…Targeted identification of target objects by derivatization is an emerging trend in research and analysis [9,10]. As a means of postcolumn derivatization, online HPLC‐DPPH assay can quickly and efficiently identify DPPH inhibitors at the level of liquid chromatography, which has been favored by several scientists [11–13].…”

Combination of temperature‐assisted ternary phase homogenous liquid–liquid extraction with deep eutectic solvent–based dispersive liquid–liquid microextraction for the extraction of phytosterols from cow milk and cream samples

“…Direct analysis of samples is preferred, but in the cases of milk and cream samples that have complex matrices this is not possible. Up to now, numerous methods including liquid–liquid extraction, solid phase extraction, homogeneous liquid–liquid extraction (HLLE), solid‐phase microextraction, dispersive liquid–liquid microextraction (DLLME, and dispersive solid‐phase extraction have been used for the extraction of various analytes from different matrices [17]. HLLE is a well‐known and extensively utilized method owing to its quickness, ease of operation, and cheapness for the extraction and preconcentration of analytes [18].…”