Derivatization for liquid chromatography/electrospray mass spectrometry: synthesis of tris(trimethoxyphenyl)phosphonium compounds and their derivatives of amine and carboxylic acids

Derivatization of neutral steroids to enhance their detection characteristics in liquid chromatography?mass spectrometry

“…Leavens and co-workers synthesized some derivatization reagents by combining the tris(trimethoxyphenyl)phosphonium (TMPP) moiety with a variety of reacting groups for amines, carboxylic acids [11], alcohols and carbonyl compounds [12]. The reagent with the S-pentafluorophenyl acetylthio ester reacts with alcohols to yield the alcohol-TMPP acetyl esters in the presence of N,N-dimethylaminopyridine (Fig.…”

Sensitivity enhancement in liquid chromatography/atmospheric pressure ionization mass spectrometry using derivatization and mobile phase additives

“…Leavens et al synthesized TMPP reagents to derivatize carboxylic acids and amines [44]. 1-Chloro-4-methylpyridinium iodide and triethylamine were used to activate the carboxyl groups.…”

Liquid chromatography coupled with inductively coupled plasma mass spectrometry in the pharmaceutical industry: selected examples

“…However, the use of chemical derivatisation to transform poorly or non-ionisable analytes easily detectable by mass spectrometry is not so common. The development of novel derivatisation methods and reagents has been the subject of several publications by our group [34][35][36]. The chemical addition of metals to a target functional group within a molecule makes the analysis of the derivative amenable to ICP-MS.…”

“…The electrophoresis gel blots were prepared as described in [34]. Prior to analysis, the gel blot strips were cut as appropriate (to fit into the ablation cell), stuck to a glass slide and placed in the ablation cell.…”