Derivatization method for the quantification of lactic acid in cell culture media via gas chromatography and applications in the study of cell glycometabolism

Zhang, Hua-Yin; Zhang, Pingping; Tan, Xin; Wang, Ze-Ze; Lian, Kaoqi; Xu, Xiangdong; Kang, Weijun

doi:10.1016/j.jchromb.2018.05.015

Cited by 13 publications

(3 citation statements)

References 35 publications

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“…More recently, the use of benzyl chloroformate (BCF) was suggested to obtain better and more reproducible results (Quehenberger et al, 2011;Tomcik et al, 2011;Zheng et al, 2013;Kloos et al, 2014;Gao and Xu, 2015;Furuhashi et al, 2018;Hoving et al, 2018;Nelson et al, 2018;Kim et al, 2019). GC-MS aided detection of lactic acid requires derivatization using alkyl chloroformates, like methyl chloroformate (MCF), ethyl chloroformate (ECF), propyl chloroformate (PCF), and isobutyl chloroformate (IBCF) (Sobolevsky et al, 2004;Qiu et al, 2007;Zampolli et al, 2007;Stefanelli et al, 2018;Zhang et al, 2018). For all molecules, GC provides separation based on affinity to the mobile gas phase vs. the stationary capillary phase.…”

Section: Analysis Of Vaginal Microbiome and Metabolomementioning

confidence: 99%

The Role of Fatty Acid Metabolites in Vaginal Health and Disease: Application to Candidiasis

et al. 2021

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Although the vast majority of women encounters at least one vaginal infection during their life, the amount of microbiome-related research performed in this area lags behind compared to alternative niches such as the intestinal tract. As a result, effective means of diagnosis and treatment, especially of recurrent infections, are limited. The role of the metabolome in vaginal health is largely elusive. It has been shown that lactate produced by the numerous lactobacilli present promotes health by limiting the chance of infection. Short chain fatty acids (SCFA) have been mainly linked to dysbiosis, although the causality of this relationship is still under debate. In this review, we aim to bring together information on the role of the vaginal metabolome and microbiome in infections caused by Candida. Vulvovaginal candidiasis affects near to 70% of all women at least once in their life with a significant proportion of women suffering from the recurrent variant. We assess the role of fatty acid metabolites, mainly SCFA and lactate, in onset of infection and virulence of the fungal pathogen. In addition, we pinpoint where lack of research limits our understanding of the molecular processes involved and restricts the possibility of developing novel treatment strategies.

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Section: Analysis Of Vaginal Microbiome and Metabolomementioning

confidence: 99%

The Role of Fatty Acid Metabolites in Vaginal Health and Disease: Application to Candidiasis

et al. 2021

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“…To assess the intensity of exercise under anaerobic metabolic conditions and prevent metabolic acidosis and fatigue from excessive exercise, lactate works as an essential biomarker among the numerous metabolites within the human body. , In recent years, numerous analytical techniques for lactate detection have been developed, such as fluorometry, colorimetry, nuclear magnetic resonance, gas chromatography, and high-performance liquid chromatography. − However, the major challenges are complex lengthy pretreatment processes, high costs, and low efficiency . When compared to other techniques, the electrochemistry technique is the preffered method for sweat monitoring due to its ability to provide real-time analysis, potential for miniaturization, relatively high sensitivity, and rapid responsiveness .…”

Section: Introductionmentioning

confidence: 99%

Nitrogen-Doped Graphene Quantum Dots Incorporated into MOF-Derived NiCo Layered Double Hydroxides for Nonenzymatic Lactate Detection in Noninvasive Biosensors

Chang,

Rinawati,

Guo

et al. 2024

ACS Appl. Nano Mater.

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Rapid interest in identifying specific biomarkers has been sparked by the development of wearable electrochemical sensors for physiological and biological monitoring via noninvasive measurement. During anaerobic metabolic circumstances, monitoring the lactate content become critical for noninvasive diagnostic of hypoxia. To improve the sensitivity of wearable sweat biosensors for detecting lactate concentrations, in this study, metal−organic framework (MOF)-derived NiCo-based layered double hydroxides (m-NiCo LDHs) with N-doped graphene quantum dots (NGQDs) decoration are designed. According to the X-ray absorption spectroscopy (XAS) analysis, the incorporation of NGQDs will alter the local electronic structure of transition metals in m-NiCo LDHs, thereby reducing the charge transfer resistance and accelerating the electron transfer kinetics during electrochemical reactions of lactate detection. After understanding the role of NGQDs in the matrix of m-NiCo LDHs, as-designed NGQD/m-NiCo LDH-based electrochemical biosensors for lactate detection displayed superior sensitivity of 62.63 ± 1.50 μA mM −1 cm −2 under an applied potential of 0.60 V (vs Ag/AgCl/3 M KCl) with the lactate concentration range of 0 to 15 mM in alkaline condition, compared to pristine NiCo LDH (16.77 ± 1.70 μA mM −1 cm −2 )-and m-NiCo LDH (45.45 ± 4.39 μA mM −1 cm −2 )-based ones.This research provides a potential electrocatalyst of GQD-modified MOF-derived LDHs for using enzyme-free electrochemical lactate sensors with reliable and stable performance in order to implement noninvasive human perspiration monitoring on wearable bioelectronics.

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“…Zhang et al proposed lactic acid as another biomarker of phthalates exposure, which was extracted from cells exposed to mono-(2-ethylhexyl) phthalate. The determination of this biomarker (potential energy source for cancer cells) could be useful in studies on mitochondrial function and glycolytic conditions in cells (Zhang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Advancement in Determination of Phthalate Metabolites by Gas Chromatography Eliminating Derivatization Step

et al. 2020

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A gas chromatography-mass spectrometry (GC-MS) method to determine polar and thermally unstable phthalate metabolites [monomethyl phthalate-MMP, monoethyl phthalate-MEP, mono-n-butyl phthalate-MnBP, mono-(2-ethylhexyl) phthalate-MEHP] has been developed. This is the first report presenting the separation of monophthalates without derivatization step and any additional equipment or special injection port. Injection parameters (temperature, pressure, time, and volume of injection), chromatographic separation (retention gap, temperature program), and MS detection/identification (working parameters, ion selection) were investigated. Mechanisms and phenomena occurring under different conditions in the GC injector were evaluated and discussed. The limits of detection (LODs) of MMP, MEP, MnBP, MEHP in the protocol were 0.049, 0.036, 0.038, and 0.029 ng (per 2 µL of injection), respectively. The response of the monophthalates was found to be linear in the tested concentration range (for MMP: 0.15-100 ng, MEP and MnBP: 0.11-100 ng, MEHP: 0.087-100 ng per 2 µL) with the coefficient of determination higher than 0.9817 and inter-day precision in the range of 1.4-5.4%. The developed method is fast, easy and repeatable. Moreover, it allows for the elimination of derivatization agents, reduction of toxic waste production and simplification of analytical procedure.

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Derivatization method for the quantification of lactic acid in cell culture media via gas chromatography and applications in the study of cell glycometabolism

Cited by 13 publications

References 35 publications

The Role of Fatty Acid Metabolites in Vaginal Health and Disease: Application to Candidiasis

The Role of Fatty Acid Metabolites in Vaginal Health and Disease: Application to Candidiasis

Nitrogen-Doped Graphene Quantum Dots Incorporated into MOF-Derived NiCo Layered Double Hydroxides for Nonenzymatic Lactate Detection in Noninvasive Biosensors

Advancement in Determination of Phthalate Metabolites by Gas Chromatography Eliminating Derivatization Step

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