Derivatization of inorganic ions in capillary electrophoresis

Padarauskas, Audrius

doi:10.1002/elps.200305407

Cited by 22 publications

(15 citation statements)

References 99 publications

(182 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A number of CE methods have been described for the analysis of anions [5][6][7]. In bare fused-silica capillaries, the EOF is greatly reduced at low pH values [8], and usually cations and anions cannot be measured in a single run.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

CE‐ESI‐MS of biological anions in plastic capillaries at high pH

et al. 2007

View full text Add to dashboard Cite

In this study, the potential of poly(methylmethacrylate) (PMMA, Plexiglas) and polyether ether ketone (PEEK) tubing for CE-ESI-MS separations of anions at high pH values was examined. A set of model compounds of biological interest was used to investigate the main operational parameters for CE-ESI-MS, such as the sheath-flow interface design, the polarity of the ionization voltage, the use of ammonia-based separation electrolytes, and the sheath liquid composition. Optimum separations and detection sensitivities in negative ESI mode were obtained using a running electrolyte of 75 mM of ammonia at pH 11 and a sheath liquid of 60:40 v/v or 75:25 v/v isopropanol/water with 0.5% v/v of ammonia. At these experimental conditions, PMMA and PEEK capillaries show good hydrolytic stabilities and lower EOF values than fused-silica columns. Better separation resolutions were obtained with PMMA capillary, but this plastic rapidly swelled and bled because of its limited chemical resistance to the sheath liquid. PMMA columns equipped with a fused-silica tip were used for a safer exposure to the sheath liquid, but the inner surface of the fused-silica tips had limited stability at pH 11. On the other hand, good separations and reproducibility on migration times and peak areas were obtained using PEEK capillaries without capillary column deterioration.

show abstract

Section: Introductionmentioning

confidence: 99%

“…In bare fused-silica capillaries, the EOF is greatly reduced at low pH values [8], and usually cations and anions cannot be measured in a single run. To measure anions, the anode must be in the outlet end of the capillary column, beyond the detector [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

CE‐ESI‐MS of biological anions in plastic capillaries at high pH

et al. 2007

View full text Add to dashboard Cite

show abstract

“…[1,26,31,32]. However, this polyatomic ion can be eliminated by the introduction of a reaction cell or a collision cell between the plasma and the quadrupole analyzer [1,26,31,32].…”

Section: Introductionmentioning

confidence: 99%

Determination of Vanadium (IV) and (V) in Southern Nevada Groundwater by Ion Chromatography-Inductively Coupled Plasma Mass Spectrometry~!2009-12-24~!2010-02-10~!2010-05-11~!

Gamage¹,

Hodge²,

Cizdziel³

et al. 2010

TOCBMJ

View full text Add to dashboard Cite

A rapid method is presented for measuring V(IV) and V(V) in groundwater, without preconcentration or complexing agents, using direct injection of the water samples (which were collected under an argon atmosphere) into an ion chromatograph-inductively coupled plasma mass spectrometer (ICPMS). The mobile phase is 1.5% v/v nitric acid, which minimizes the buildup of solids in the ultrasonic nebulizer, the torch, and on the entrance cones of the ICPMS -a problem with methods using organic complexing agents and inorganic salts. The result is much less instrument downtime for cleaning the affected parts. Limits of detection are 0.02 g L -1 for V(IV) and 0.06 g L -1 for V(V). The pentavalent form of vanadium represents 98% to 99% of the vanadium in these Southern Nevada groundwater samples and ranges from 3.7±0.1 g L -1 to 82.1±0.5 g L -1 . The remaining few percent is V(IV). Total vanadium was determined by ICPMS for a mass-balance comparison.

show abstract

“…The development in CE determination of inorganic cations as their complexes is reviewed. [27][28][29][30][31][32][33][34][35] Based on precolumn complexation with a lacunary Keggin-type [PW11O39] 7-anion, we propose a new approach to the simultaneous determination of metal ions belonging to the same group in a periodic table, such as Sb(III) and Bi(III), 36 Sc(III) and Y(III), 37 or Zr(IV) and Hf(IV). 38 The determination of toxic metal ions is often required for environmental monitoring.…”

Section: Introductionmentioning

confidence: 99%

Selective Determination of Cadmium(II) from Divalent Metal Ions in Environmental Samples by Capillary Electrophoresis Using In-capillary Complexation with a Lacunary Keggin-type [PW11O39]7– Complex

2007

View full text Add to dashboard Cite

IntroductionRecently, capillary electrophoresis (CE) has proved to be efficient for the separation and detection of transition-metal cations. For the CE determination of such non-UV absorbing metal ions, indirect UV detection is widely used. [1][2][3][4] To improve the detection sensitivities, a direct UV detection method is proposed using pre-and in-capillary complexation with various organic and inorganic ligands. 5-26The development in CE determination of inorganic cations as their complexes is reviewed. [27][28][29][30][31][32][33][34][35] Based on precolumn complexation with a lacunary 7-anion, we propose a new approach to the simultaneous determination of metal ions belonging to the same group in a periodic table, such as Sb(III) and Bi(III), 36 Sc(III) and Y(III), 37 or Zr(IV) and Hf(IV). 38 The determination of toxic metal ions is often required for environmental monitoring. However, the application of such complexation methods to seawater analysis is difficult, owing to high concentrations of matrix ions. 39 The CE determination of toxic metal ions in seawater is a challenging area in environmental chemistry.The present study focused on the development of a simple and sensitive CE method for the determination of Cd(II) in environmental samples. We found that trace amounts of Cd(II) reacted spontaneously with [PW11O39] 7-to form the Keggin-type [P(CdW11)O39] 5-anion. Because the ternary Keggin anion possesses high molar absorptivities in the UV region, the use of [PW11O39] 7-as a complexing reagent allows direct UV detection with a satisfactory detection limit. In this study, we developed a new in-capillary complexation method for the determination of Cd(II) in river-water and seawater samples. To our knowledge, this is the first report on the sensitive CE determination of Cd(II) in seawater. Experimental InstrumentationA P/ACE MDQ system (Beckman Instruments) equipped with a built-in UV diode-array detector was used for CE measurements. A fused-silica capillary (GL Sciences, Tokyo, Japan) with 75 μm i.d. and 70 cm total length was used as a separation and reaction capillary. The 31 P (202.46 MHz) NMR spectra were recorded on a Bruker Model AVANCE 500 spectrometer in a 5 mm diameter NMR tube with a concentric capillary containing D2O for an instrumental lock. Chemical shifts were expressed in parts per million with respect to 85% (v/v) H3PO4. The 31 P NMR measurements were made at 25 ± 0.1˚C. UV-visible spectra were recorded with a Hitachi Model U-3000 spectrophotometer. CE procedureFor in-capillary complexation, the capillary was filled with a running electrolyte consisting of 0.20 mM [PW11O39] 7-and 0.10 M malonate buffer (pH 3.0). Samples were injected at the cathodic end of the capillary by applying a pressure of 3.4 × 10 3 Pa for 10 -20 s. Throughout the work, direct UV detection was made at 250 nm, and the capillary temperature was held at 30˚C. ChemicalsAll chemicals were of analytical reagent grade and used as received. , which possesses high molar absorbtivities in the UV region, is formed in...

show abstract

Derivatization of inorganic ions in capillary electrophoresis

Cited by 22 publications

References 99 publications

CE‐ESI‐MS of biological anions in plastic capillaries at high pH

CE‐ESI‐MS of biological anions in plastic capillaries at high pH

Determination of Vanadium (IV) and (V) in Southern Nevada Groundwater by Ion Chromatography-Inductively Coupled Plasma Mass Spectrometry~!2009-12-24~!2010-02-10~!2010-05-11~!

Selective Determination of Cadmium(II) from Divalent Metal Ions in Environmental Samples by Capillary Electrophoresis Using In-capillary Complexation with a Lacunary Keggin-type [PW11O39]7– Complex

Contact Info

Product

Resources

About