Dermal exosomes containing miR-218-5p promote hair regeneration by regulating β-catenin signaling

Abstract

The progression in the hair follicle cycle from the telogen to the anagen phase is the key to regulating hair regrowth. Dermal papilla (DP) cells support hair growth and regulate the hair cycle. However, they gradually lose key inductive properties upon culture. DP cells can partially restore their capacity to promote hair regrowth after being subjected to spheroid culture. In this study, results revealed that DP spheroids are effective at inducing the progression of the hair follicle cycle from telogen to anagen compared with just DP cell or minoxidil treatment. Because of the importance of paracrine signaling in this process, secretome and exosomes were isolated from DP cell culture, and their therapeutic efficacies were investigated. We demonstrated that miR-218-5p was notably up-regulated in DP spheroid–derived exosomes. Western blot and immunofluorescence imaging were used to demonstrate that DP spheroid–derived exosomes up-regulated β-catenin, promoting the development of hair follicles.

Neural progenitor cell-derived nanovesicles promote hair follicle growth via miR-100

“…However, DPC is not a reliable and expandable cell source for large-scale production of EVs. Furthermore, Cheng et al revealed that miR-218-5p-overexpressed exosomes accelerated the onset of anagen, and DPC spheroid culture provided a benificial avenue for cell therapy [ 31 ]. The authors also mentioned that the lack of hormone-related or disease-related hair loss model needed to be filled up in the future.…”

Neural progenitor cell-derived nanovesicles promote hair follicle growth via miR-100

“…However, DPC is not a reliable and expandable cell source for large-scale production of EVs. Furthermore, Cheng et al revealed that miR-218-5p-overexpressed exosomes accelerated the onset of anagen, and DPC spheroid culture provided a beni cal avenue for cell therapy [31] . The authors also mentioned that the lack of hormone-related or disease-related hair loss model needed to be lled up in the future.…”

Extracellular Vesicles: “Stealth Transport Aircrafts” for Drugs

“…A number of studies have shown that EVs derived from mesenchymal stem cells (MSCs) can be used for stem cell replacement therapy [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]. In most cases, it is not clear which component of the unmodified EVs exerts curative effects.…”

“…MSC-derived EVs reduce radiation-induced lung injury through miRNA-214-3p [13]. Replacing autologous cells with EVs derived from hair follicle papillary cell spheres can promote hair growth [14]. Human umbilical cord mesenchymal stem cell-derived exosomes (UMSC-Exo) can inhibit pyrolysis and repair muscle ischemic injury by releasing circular RNA circHIPK3 [15].…”

“…Because they have anti-inflammatory, anti-apoptotic and anti-microbial capability, and promote angiogenesis and the repair and regeneration of damaged tissues. As mentioned above, EVs derived from MSCs have been widely used in the treatment of central nervous system diseases, cardiovascular diseases, bone and cartilage damage repair and regeneration, wound repair, and other organ damage repair and regeneration [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21].…”