Dermal penetration of creatine from a face-care formulation containing creatine, guarana and glycerol is linked to effective antiwrinkle and antisagging efficacy in male subjects

Peirano, Reto I.; Achterberg, Volker; Düsing, Hans-Jürgen; Akhiani, Mehdi; Koop, Urte; Jaspers, Sören; Krüger, Andrea; Schwengler, Helge; Hamann, Tina; Wenck, Horst; Stäb, Franz; Gallinat, Stefan; Blatt, Thomas

doi:10.1111/j.1473-2165.2011.00579.x

Cited by 9 publications

(8 citation statements)

References 30 publications

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“…Recent findings indicate that urea is a highly active substance with specific gene‐regulating activities which affect mRNA expression of certain genes involved in keratinocyte differentiation. Creatine helps to supply energy to cells increasing the formation of adenosine triphosphate (ATP) improving mitochondrial functions. Palmitoyl tripeptide‐38 is an amino acid chain which has demonstrated the capacity to increase the production of dermal and epidermal components such as collagen, fibronectin, and hyaluronic acid .…”

Section: Introductionmentioning

confidence: 99%

Biorevitalizing effect of a novel facial serum containing apple stem cell extract, pro‐collagen lipopeptide, creatine, and urea on skin aging signs

Sanz

Campos

Milani

et al. 2015

J of Cosmetic Dermatology

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SummaryBackground: Epithelial regeneration in skin is achieved by the constant turnover and differentiation of keratinocytes. Epidermal and dermal stem cells compartments are fundamental for the continuous renewal of the skin. Adult stem cells are the unique source for skin tissue renewal. Plants have stem cells and plant derived stem cell extracts are now used in topical products for their potential anti-ageing and antiwrinkle effects. A new dermocosmetic product containing apple stem cell extract, urea, creatine and palmitoyl tripeptide-38 (Ureadin Fusion Serum Lift Antiarrugas, ISDIN S.A), has been recently developed to target different aspects involved in skin aging. Study aim: To assess in vitro the effects of this new serum on the metabolic functions of human senescent fibroblasts and in vivo the anti-aging effects by clinical and instrumental evaluation. Methods: We evaluated the effects of the serum on the mitochondrial ROS (reactive oxygen species) production in human senescent cultured fibroblasts measured at 0.1% and 1% using the Mitoread AntiOx mtROS method. In addition we evaluated the anti-ageing in vivo effect of this new serum applied on the face twice daily for 28 consecutive days and assessed by clinical and instrumental evaluation in 32 women with sensitive skin bearing wrinkles on crow's feet. Results: The tested serum both at 0.1% and 1% induces a significant increase in 02 consumption, cellular ATP level and a reduction in extra-cellular lactate concentration. The product reduces also significantly the mitochondrial ROS production. The clinical study shows a relevant anti-wrinkle effect in 71% of the treated women with visible effects in 68% of the subjects as soon as 7 days of treatment. A significant increase in dermal density and skin elasticity was also observed. Conclusion: The use of this novel anti-aging serum demonstrated a significant improvement of aging skin signs with first visible results achieved after one week of use. The product seemed to optimize the metabolic functions in human senescent cultured fibroblast restoring a more efficient cell metabolism therefore contributing to the anti-aging properties of the product.

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Section: Introductionmentioning

confidence: 99%

Biorevitalizing effect of a novel facial serum containing apple stem cell extract, pro‐collagen lipopeptide, creatine, and urea on skin aging signs

Sanz

Campos

Milani

et al. 2015

J of Cosmetic Dermatology

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“…10 Other small molecules produced by LB244R® that may affect aging include taurine and creatine, both of which can alleviate aging. 21 The microbiome plays a role in aging as well. 3 Centenarians have higher levels of Lactobacillus and additionally, Lactobacillus present on skin reduces with age.…”

Section: Discussionmentioning

confidence: 99%

A proof of concept: Clinical anti‐aging efficacy and safety of Lactiplantibacillus plantarumLB244R® applied topically in a double‐blinded placebo‐controlled study

Elvebakken,

Christensen,

Vedel

et al. 2024

J of Cosmetic Dermatology

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BackgroundWith the increasing age of the westernized population, there is also increasing economic and aesthetic interest in reducing the signs of skin aging. Additionally, the physical aspect of aging can be displeasing and have detrimental effects psychologically in individuals. Probiotics have shown potential as anti‐aging agents, albeit proper studies are needed to confirm their potential.AimsProving that Lactiplantibacillus plantarum LB244R® could alleviate aging signs relative to its placebo vehicle.Patients/MethodsIn total, 46 subjects were randomly assigned either the ointment with live bacteria, L. plantarum LB244R® or its vehicle ointment, and had to use the assigned ointment twice daily for 56 days. On Day 0, Day 28, and Day 56 subepidermal low echogenic band (SLEB) thickness, dermal density, skin firmness and elasticity, skin hydration, transepidermal water loss (TEWL), skin pH, collagen fiber visualization using confocal microscopy, Crow's feet, spot score, skin smoothness, and complexion radiance were assessed by dermatologists.ResultsAll parameters except TEWL improved relative to their baseline (D0) for the active group. L. plantarum LB244R® improved SLEB thickness, dermal density, skin elasticity, skin hydration, and Crow's feet wrinkle score relative to the placebo vehicle ointment.ConclusionThe study demonstrates an anti‐aging effect of L. plantarum LB244R® for topical skin use in the first double‐blinded, vehicle‐ointment placebo‐controlled clinical study.

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“…In previous studies, Peirano et al ( 2011) [51] demonstrated that guarana presented 23% higher cellular esterase activity than formulations without guarana, exerting a vitalizing effect on skin fibroblasts.…”

Section: Cytotoxicity Studiesmentioning

confidence: 95%

“…It is present in several cosmetic products, due to its antimicrobial [39][40] and antioxidant activity [34-36, 39, 41-50]. Due to the high content of alkaloids in guarana, extracts are added to products for the treatment of gynoid lipodystrophy and to anti-aging creams [51][52]. Given the importance of guarana and the increasing use of its seeds, there has been increased interest in the quality of the products containing this compound because its chemical structure is predominantly unsaturated and susceptible to oxidation [53].…”

Section: Introductionmentioning

confidence: 99%

Potential Use of Guarana-Loaded Liposomes in Topical Applica-Tions: Toxicity Profile Assessment Using Different Skin Cell Lines

Roggia¹,

Gomes²,

Dalcin³

et al. 2023

Preprint

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The objective of this study was to analyze the in vitro stability and toxicity of liposomes containing guarana in skin cell lines. The liposomes were produced by the reverse phase evaporation method containing 1 mg/mL guarana. The stability of the liposomes was evaluated by physical-chemical parameters for up to 90 d using three different storage conditions. The cytotoxicity of guarana (GL), liposomes (B-Lip), and guarana-loaded liposomes (G-Lip) was evaluated on spontaneously immortalized human keratinocyte cell lines (HaCaT), murine swiss albino fibroblasts (3T3), and human fibroblast (1BR.3.G). The evaluation was performed using cellular viability analysis. The techniques used were 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red capturing (NRU) and the analyses were conducted after 24, 48, and 72 h exposure of these cells to the different treatments. The G-Lip exhibited physical-chemical stability for 60 d when the samples were stored in a refrigerator. The GL, B-Lip, and G-Lip demonstrated low cytotoxicity in the three cell cultures tested since a low reduction in cell viability was only observed at the highest concentrations. In addition, greater cell damage was observed for B-Lip; however, guarana protects cells from this damage. Thus, G-Lip structures can be considered as promising systems for topical applications.

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Dermal penetration of creatine from a face-care formulation containing creatine, guarana and glycerol is linked to effective antiwrinkle and antisagging efficacy in male subjects

Abstract: In summary, creatine represents a beneficial active ingredient for topical use in the prevention and treatment of human skin aging.

Cited by 9 publications

References 30 publications

Biorevitalizing effect of a novel facial serum containing apple stem cell extract, pro‐collagen lipopeptide, creatine, and urea on skin aging signs

Biorevitalizing effect of a novel facial serum containing apple stem cell extract, pro‐collagen lipopeptide, creatine, and urea on skin aging signs

A proof of concept: Clinical anti‐aging efficacy and safety of Lactiplantibacillus plantarumLB244R® applied topically in a double‐blinded placebo‐controlled study

Potential Use of Guarana-Loaded Liposomes in Topical Applica-Tions: Toxicity Profile Assessment Using Different Skin Cell Lines

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