Dermally Non-invasive Suctioning Technique for Painless Serial Sampling of Interstitial Fluid (ISF) in Newborn Infants 1525

Hansen, Ingrid; Håkansson, K; Polberger, Staffan; Svenningsen, Nils W.; Svedman, P

doi:10.1203/00006450-199804001-01547

Cited by 4 publications

(2 citation statements)

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“…Each sampling produced suf®cient volumes for measuring a number of substances of interest in diabetes research. Even in the diabetic subjects, there were no complications and epidermal regeneration was rapid; ®ndings that further con®rm the ef®ciency of the dermal protective-regenerative response at this access site [4,5]. Taken together, the observations clearly validate use of the technique in diabetic subjects and corroborate earlier ®ndings in this respect [4].…”

Section: Discussionsupporting

confidence: 80%

Skin mini-erosion technique for monitoring metabolites in interstitial fluid: its feasibility demonstrated by OGTT results in diabetic and non-diabetic subjects

Svedman

Samra

Clark

et al. 1999

Scandinavian Journal of Clinical and Laboratory Investigation

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The aim of this study was to evaluate a new interstitial fluid (IF) sampling technique and its application in diabetology. IF and venous whole blood were sampled serially during an oral glucose tolerance test (OGTT) on days 1 and 3 after formation of a mini-erosion in eight non-diabetic controls and eight Type 2 diabetic subjects. Glucose, lactate, glycerol, 3-hydroxybutyrate and insulin were assayed in IF and plasma. With solitary exceptions, the IF sample volumes were in excess of those required for measurement of all five substances. However, mean IF sampling rates differed significantly both between the non-diabetic and diabetic groups and between days 1 and 3 (p < 0.001 in all cases). In both groups, the OGTT curves of glucose, glycerol and 3-hydroxybutyrate were similar to the respective curves for plasma, whereas for lactate concentrations they were markedly greater in IF than in plasma (40% and 93% in the control group and 57% and 100% greater in the diabetic group on days 1 and 3, respectively). The reverse was true of insulin concentrations in the non-diabetic group, which were 57% and 74% lower in IF than in plasma on days 1 and 3, respectively. In the non-diabetic group, the baseline (pre-OGTT) insulin level in IF increased from 49 +/- 22% (SD) of that in plasma on day 1 to 74 +/- 19% of that in plasma on day 3 (p=0.005). Sampling site re-epithelialization was rapid. In conclusion, the feasibility of transdermal sampling of IF via a skin mini-erosion has been demonstrated in both diabetic and non-diabetic subjects.

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Section: Discussionsupporting

confidence: 80%

Skin mini-erosion technique for monitoring metabolites in interstitial fluid: its feasibility demonstrated by OGTT results in diabetic and non-diabetic subjects

Svedman

Samra

Clark

et al. 1999

Scandinavian Journal of Clinical and Laboratory Investigation

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“…The procedure for obtaining the erosion that is outlined below was developed in a preliminary study including 12 infants [9] . A suction cup (Epiport, Sweden) was applied, without prior topical disinfection, on the abdominal skin below and lateral to the umbilicus of the infant and a negative pressure of 220 mm Hg was applied for 45 min.…”

Section: Formation Of a Mini-erosion And Sampling Of If And Bloodmentioning

confidence: 99%

Transdermal Suctioning Technique for Serial Sampling of Interstitial Fluid in Newborn Infants

Hansen‐Pupp

Håkansson²,

Polberger³

et al. 2005

Neonatology

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Overview: Use of a suction-induced epidermal mini-erosion for serial sampling of dermal interstitial fluid (IF) in 16 newborn infants with gestational age ranging from 24 to 42 weeks is reported. Results: The mini-erosion formed reproducibly and electron microscopy showed that the split was located within the epidermis. IF was sampled serially by suctioning during 1–3 days without signs of stress or pain. IF sample volumes (10–50 µl) did not decrease with time. Glucose values in IF and blood were correlated (r_s = 0.542; p = 0.000). IF glucose was lower than blood glucose on all 3 days. The epidermis regenerated within 4 ± 0.9 days (mean, SD) after sampling was terminated. There were no complications. Conclusion: Serial transdermal sampling was performed without stress or pain in newborn infants. Systemic and temporal differences between glucose measured in IF and blood were observed.

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Plasma proteins in a standardised skin mini-erosion (I): permeability changes as a function of time

Svedman

Ryan

et al. 2002

BMC Dermatol

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Dermally Non-invasive Suctioning Technique for Painless Serial Sampling of Interstitial Fluid (ISF) in Newborn Infants 1525

Cited by 4 publications

References 0 publications

Skin mini-erosion technique for monitoring metabolites in interstitial fluid: its feasibility demonstrated by OGTT results in diabetic and non-diabetic subjects

Skin mini-erosion technique for monitoring metabolites in interstitial fluid: its feasibility demonstrated by OGTT results in diabetic and non-diabetic subjects

Transdermal Suctioning Technique for Serial Sampling of Interstitial Fluid in Newborn Infants

Plasma proteins in a standardised skin mini-erosion (I): permeability changes as a function of time

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