Dermaseptin-S1 decreases<i>Candida albicans</i>growth, biofilm formation and the expression of hyphal wall protein 1 and aspartic protease genes

Belmadani, Amine; Semlali, Abdelhabib; Rouabhia, Mahmoud

doi:10.1111/jam.13745

Cited by 34 publications

(17 citation statements)

References 43 publications

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“…It is noteworthy to mention here that engineered antifungal peptide histatin-5 altered the proteolysis activity of Sap2 and Sap9 and enhanced the antifungal activity ( Ikonomova et al, 2018 ). Similarly, dermaseptin-S1, another antimicrobial peptide, has been reported for its inhibitory effect on Saps ( sap1, sap2, sap3, sap9 , and sap10 ) along with growth, biofilm formation, morphological transition in C. albicans ( Belmadani et al, 2018 ). Further, morphological transition from yeast to hyphae is one of the important virulence factors of C. albicans.…”

Section: Discussionmentioning

confidence: 98%

Synergistic Effect of Quinic Acid Derived From Syzygium cumini and Undecanoic Acid Against Candida spp. Biofilm and Virulence

et al. 2018

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In recent decades, fungal infections have incredibly increased with Candida genus as the major cause of morbidity and mortality in hospitalized and immunocompromised patients. Most of the Candida species are proficient in biofilm formation on implanted medical devices as well as human tissues. Biofilm related Candida infections are very difficult to treat using common antifungal agents owing to their increased drug resistance. To address these issues, the present study investigated the antibiofilm and antivirulent properties of Syzygium cumini derived quinic acid in combination with known antifungal compound undecanoic acid. Initially, antibiofilm potential of S. cumini leaf extract was assessed and the active principles were identified through gas chromatography and mass spectrometry analysis. Among the compounds identified, quinic acid was one of the major compounds. The interaction between quinic acid and undecanoic acid was found to be synergistic in the Fractional inhibitory concentration index (≤0.5). Results of in vitro assays and gene expression analysis suggested that the synergistic combinations of quinic acid and undecanoic acid significantly inhibited virulence traits of Candida spp. such as the biofilm formation, yeast-to-hyphal transition, extracellular polymeric substances production, filamentation, secreted hydrolases production and ergosterol biosynthesis. In addition, result of in vivo studies using Caenorhabditis elegans demonstrated the non-toxic nature of QA-UDA combination and antivirulence effect against Candida spp. For the first time, synergistic antivirulence ability of quinic acid and undecanoic acid was explored against Candida spp. Thus, results obtained from the present study suggest that combination of phytochemicals might be used an alternate therapeutic strategy for the prevention and treatment of biofilm associated Candida infection.

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Section: Discussionmentioning

confidence: 98%

Synergistic Effect of Quinic Acid Derived From Syzygium cumini and Undecanoic Acid Against Candida spp. Biofilm and Virulence

et al. 2018

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“…Indeed, previous studies have found leakage and morphological alterations in the artificial bacterial membrane after treatment with fluorescent-labelled dermaseptins [ 21 , 22 ]. More recently, using the electron microscopy, a dermaseptin peptide, DS1, was found to distort the cell wall surface, proposing that the cytolysis or cell membrane disruption of C. albicans eventually cause cell death [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Biological Activities of Cationicity-Enhanced and Hydrophobicity-Optimized Analogues of an Antimicrobial Peptide, Dermaseptin-PS3, from the Skin Secretion of Phyllomedusa sauvagii

Tan

Chen

et al. 2018

Toxins

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The skin secretions of the subfamily Phyllomedusinae have long been known to contain a number of compounds with antimicrobial potential. Herein, a biosynthetic dermaseptin-precursor cDNA was obtained from a Phyllomedusa sauvagii skin secretion-derived cDNA library, and thereafter, the presence of the mature peptide, namely dermaseptin-PS3 (DPS3), was confirmed by LC–MS/MS. Moreover, this naturally occurring peptide was utilized to design two analogues, K5, 17-DPS3 (introducing two lysine residues at positions 5 and 17 to replace acidic amino acids) and L10, 11-DPS3 (replacing two neutral amino acids with the hydrophobic amino acid, leucine), improving its cationicity on the polar/unipolar face and hydrophobicity in a highly conserved sequence motif, respectively. The results in regard to the two analogues show that either increasing cationicity, or hydrophobicity, enhance the antimicrobial activity. Also, the latter analogue had an enhanced anticancer activity, with pretreatment of H157 cells with 1 µM L10, 11-DPS3 decreasing viability by approximately 78%, even though this concentration of peptide exhibited no haemolytic effect. However, it must be noted that in comparison to the initial peptide, both analogues demonstrate higher membrane-rupturing capacity towards mammalian red blood cells.

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“…Each exposure condition (CCS, NF, NR, and Ctrl) was performed in a separate exposure chamber to avoid culture cross-contamination. At the end of each exposure regime (2 or 3 days), C. albicans growth was determined by MTT assay, as previously reported [15]. Results were reported as means ± SD, n = 5.…”

Section: Methodsmentioning

confidence: 99%

“…Following each exposure, cultures were incubated for 60 min before the culture medium was refreshed. Following the second exposure, C. albicans cultures were incubated for 16 h at 37 °C and subsequently used to extract total RNA, as we previously reported [15]. The RNA (1 μg of each sample) was reverse transcribed into cDNA by means of the iScript cDNA Synthesis kit (Bio-Rad) and used for quantitative PCR (qPCR).…”

Section: Methodsmentioning

confidence: 99%

E-Cigarettes Increase Candida albicans Growth and Modulate its Interaction with Gingival Epithelial Cells

Alanazi

Semlali

Chmielewski

et al. 2019

IJERPH

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Electronic cigarette (e-cigarette) vapor comes in contact with the different constituents of the oral cavity, including such microorganisms as Candida albicans. We examined the impact of e-cigarettes on C. albicans growth and expression of different virulent genes, such as secreted aspartic proteases (SAPs), and the effect of e-cigarette vapor-exposed C. albicans on gingival epithelial cell morphology, growth, and lactate dehydrogenase (LDH) activity. An increase in C. albicans growth was observed with nicotine-rich e-cigarettes compared with non-exposed cultures. Following exposure to e-cigarette vapor, C. albicans produced high levels of chitin. E-cigarettes also increased C. albicans hyphal length and the expression of SAP2, SAP3, and SAP9 genes. When in contact with gingival epithelial cells, e-cigarette-exposed C. albicans adhered better to epithelial cells than the control. Indirect contact between e-cigarette-exposed C. albicans and gingival epithelial cells led to epithelial cell differentiation, reduced cell growth, and increased LDH activity. Overall, results indicate that e-cigarettes may interact with C. albicans to promote their pathogenesis, which may increase the risk of oral candidiasis in e-cigarette users.

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Dermaseptin-S1 decreasesCandida albicansgrowth, biofilm formation and the expression of hyphal wall protein 1 and aspartic protease genes

Abstract: These data provide new insight into the efficacy of DS1 against C. albicans and its potential for use as an antifungal therapy.

Cited by 34 publications

References 43 publications

Synergistic Effect of Quinic Acid Derived From Syzygium cumini and Undecanoic Acid Against Candida spp. Biofilm and Virulence

Synergistic Effect of Quinic Acid Derived From Syzygium cumini and Undecanoic Acid Against Candida spp. Biofilm and Virulence

Biological Activities of Cationicity-Enhanced and Hydrophobicity-Optimized Analogues of an Antimicrobial Peptide, Dermaseptin-PS3, from the Skin Secretion of Phyllomedusa sauvagii

E-Cigarettes Increase Candida albicans Growth and Modulate its Interaction with Gingival Epithelial Cells

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