Dermatophytes Species Isolated of HIV-Infected Patients Identified by ITS-RFLP and ITS Region Sequencing from Triangulo Mineiro, Minas Gerais State of Brazil

Abstract

Dermatomycoses are a group of diseases worldwide distributed and affect skin, hair and nails. Several fungal species can be envolved and keratinophilic fungi belonging to the genera Trichophyton, Microsporum and Epidermophyton are very common. These infections are globally distributed and their incidence has progressively increased. Despite their high incidence, few studies have been carried out in HIV-infected patients, regarding frequency, clinical presentations, and species identification. This study aimed to evaluate some epidemiological and clinical aspects of dermatophytosis in HIV-infected patients and to attempt phenotypical and molecular characteristics of their agents. Of 398 patients included, 306 were HIV-infected. Clinical data and samples of skin, hair and nail lesions were simultaneously obtained. The dermatophytes yielded in culture were phenotypically identified and evaluated by ITS-RFLP and ITS (Internal Transcribed Space) sequencing. Dermatophytes frequency in HIV-infected patients with cutaneous lesions was 11.76% similar to the 15.22% observed in non-HIV individuals. Trichophyton rubrum was the most common isolated species in both groups. Throught ITS-RFLP, 29/32 (90.63%) of dermatophytes isolates were identified at the species level. From the 10 isolates randomly selected for ITS sequencing, seven confirmed the ITS-RFLP results while three Trichophyton sp. were not identified as dermatophytes. The identification of these species in HIV-infected patients is highly desirable in order to improve the knowledge of their fre-How to cite this paper: Bragine-Ferreira, T., de Lima-Júnior, L.

Mucocutaneous mycoses in people living with human immunodeficiency virus in Indonesia

“…Contradictory to these results, Otike-Odibi et al found that dermatophytosis in HIV-infected patients is often encountered in people with a secondary educational level, and Bragine-Ferreira et al found only a small proportion of dermatophyte infection in PLHIV with a high educational level. 10,13 However, there is a record of similar results with this study, found by Krzyściak et al, who reported that a majority of the population with a lower education is infected with Malassezia. 14 Malassezia infections can be triggered by several factors, such as a hot and humid environment and overcrowded areas.…”

DNA topoisomerase 2 gene polymorphism in dermatophytes

“…The dermatophytes are a group of keratinophilic ascomycetes from the family Arthrodermataceae . Traditional procedures for fungal systematics based on macro and micro‐morphology of fungi in culture media, physiological and biological traits and ecological niche have limited reliability for assigning dermatophytes to species level or inferring their relationships 1‐3 . Therefore, phylogeny of the group has been studied by molecular methods.…”

Hongos Levaduriformes causantes de Onicomicosis en hombres adultos que residen en la Ciudad de Ambato – Ecuador

“…Análisis estadístico: Se ubica en un enfoque cualicuantitativo, cualitativo ya que se fundamentó en la revisión de la literatura relacionada para comprender el fenómeno estudiado, y es cuantitativo porque se utilizó la recolección y análisis estadístico para probar la hipótesis, se calculó los porcentajes y frecuencias para determinar el nivel de cumplimiento de la investigación (Bragine-Ferreira et al, 2019), (Gnat et al, 2020).…”