Dermo-epidermal organotypic cultures for in vitro evaluation of skin irritation and corrosion

Agudelo, César Alveiro Montoya; Becerra, Natalia; Vergara, Jaime; Correa, Luis Alfonso; Estrada, Sebastián; Múnera, Luz Marina Restrepo

doi:10.1016/j.tiv.2019.104657

Cited by 9 publications

(2 citation statements)

References 17 publications

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“…The plasma-derived hydrogels were prepared according to the protocol described by Gaviria et al [54], by mixing human plasma (67 % v/v), normal saline solution (18 % v/v), tranexamic acid (1.4 % v/v), and different concentrations of Nps (0-4 mg/mL). The gelation was induced by the addition of 1 % CaCl 2 solution (14 % v/v) and aging for 30 min at 37 • C. The hydrogels obtained were named PS, PG0.1, PG0.5, and PG1, according to the Nps type added.…”

Section: Preparation and Characterization Of Plasma-derived Hydrogelsmentioning

confidence: 99%

Modulating the water behavior, microstructure, and viscoelasticity of plasma-derived hydrogels by adding silica nanoparticles with tailored chemical and colloidal properties

Galeano-Duque

Sharma

Mesa

2023

Materials Today Communications

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Section: Preparation and Characterization Of Plasma-derived Hydrogelsmentioning

confidence: 99%

Modulating the water behavior, microstructure, and viscoelasticity of plasma-derived hydrogels by adding silica nanoparticles with tailored chemical and colloidal properties

Galeano-Duque

Sharma

Mesa

2023

Materials Today Communications

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“…Gelatin and fibrin − are popular noncollagen-based biomaterials for the dermal matrix biofabrication of 3D organotypic skin models. As a collagen derivative, gelatin displays relatively low antigenicity while retaining the biocompatibility and biodegradability properties of the collagen .…”

Section: Ecm-based Organotypic Skin Models Using Conventional Static ...mentioning

confidence: 99%

Advancements in Extracellular Matrix-Based Biomaterials and Biofabrication of 3D Organotypic Skin Models

Phang

Basak

Teh

et al. 2022

ACS Biomater. Sci. Eng.

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Over the last decades, three-dimensional (3D) organotypic skin models have received enormous attention as alternative models to in vivo animal models and in vitro twodimensional assays. To date, most organotypic skin models have an epidermal layer of keratinocytes and a dermal layer of fibroblasts embedded in an extracellular matrix (ECM)-based biomaterial. The ECM provides mechanical support and biochemical signals to the cells. Without advancements in ECM-based biomaterials and biofabrication technologies, it would have been impossible to create organotypic skin models that mimic native human skin. In this review, the use of ECM-based biomaterials in the reconstruction of skin models, as well as the study of complete ECM-based biomaterials, such as fibroblasts-derived ECM and decellularized ECM as a better biomaterial, will be highlighted. We also discuss the benefits and drawbacks of several biofabrication processes used in the fabrication of ECM-based biomaterials, such as conventional static culture, electrospinning, 3D bioprinting, and skin-on-achip. Advancements and future possibilities in modifying ECM-based biomaterials to recreate disease-like skin models will also be highlighted, given the importance of organotypic skin models in disease modeling. Overall, this review provides an overview of the present variety of ECM-based biomaterials and biofabrication technologies available. An enhanced organotypic skin model is expected to be produced in the near future by combining knowledge from previous experiences and current research.

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Essential Oil Components Incorporated Emulsion Hydrogels for Eradicating Dermatophytosis Caused by Pathogenic Fungi Trichophyton mentagrophytes and Microsporum canis

Preman,

Amin,

Sanjeeva

et al. 2024

Adv Healthcare Materials

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Dermatophytosis is a prevalent fungal infection and public health burden, majorly caused by the attack of zoophilic fungi genera of Trichophyton and Microsporum. Among them, T. mentagrophytes and M. canis are the dominating pathogens that cause dermatophytosis in humans. Though anti‐fungal treatments are available, the widespread drug resistance and minimal efficacy of conventional therapies cause recurring infections. In addition, prolonged anti‐fungal medications induce several systemic side effects, including hepatotoxicity and leucopenia. The anti‐dermatophytic formulation of biocompatible essential oil components (EOCs) is attractive due to their highly potent anti‐dermatophytic action. Herein, two EOCs, Eugenol (EU) and Isoeugenol (IU), incorporated emulsion hydrogel (EOCs‐EHG) synthesized from hydroxypropylmethyl cellulose and poly(ethylene glycol) methyl ether methacrylate. The cytocompatibility of the hydrogels is confirmed by treating them with fibroblast and keratinocyte cell lines. The EOCs‐EHG demonstrated pH and temperature‐responsive sustained release of entrapped EOCs and inhibited fungal spore germination. T. mentagrophytes and M. canis biofilms are eradicated at a minimal inhibitory concentration of 2 µg mL−1 each of EU and IU. The in vivo anti‐dermatophytic activity of EOCs‐EHG is confirmed in dermatophyte‐infected Wistar albino rat models. The topical application of EOCs‐EHG demonstrated complete infection eradication and facilitated skin regeneration, emphasizing the therapeutic potential of EOCs‐EHG against dermatophytosis.

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Dermo-epidermal organotypic cultures for in vitro evaluation of skin irritation and corrosion

Cited by 9 publications

References 17 publications

Modulating the water behavior, microstructure, and viscoelasticity of plasma-derived hydrogels by adding silica nanoparticles with tailored chemical and colloidal properties

Modulating the water behavior, microstructure, and viscoelasticity of plasma-derived hydrogels by adding silica nanoparticles with tailored chemical and colloidal properties

Advancements in Extracellular Matrix-Based Biomaterials and Biofabrication of 3D Organotypic Skin Models

Essential Oil Components Incorporated Emulsion Hydrogels for Eradicating Dermatophytosis Caused by Pathogenic Fungi Trichophyton mentagrophytes and Microsporum canis

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