Dermoscopic criteria associated with
            <i>
              <scp>BRAF</scp>
            </i>
            and
            <i>
              <scp>NRAS</scp>
            </i>
            mutation status in primary cutaneous melanoma

Pozzobon, Flavia Carolina; Puig-Butillé, Joan Antón; González-Álvarez, Tatiana; Carrera, Cristina; Aguilera, Paula; Alós, Llúcia; Badenas, Cèlia; Grichnik, James M.; Malvehy, Josep; Puig, Susana

doi:10.1111/bjd.13069

Cited by 27 publications

(40 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dermoscopic features have been associated with genetic alteration, while few studies have been conducted to identify a correlation between BRAF mutational status of melanomas and their dermoscopic or confocal microscopy substrates …”

Section: Introductionmentioning

confidence: 99%

Association between dermoscopic and reflectance confocal microscopy features of cutaneous melanoma with BRAF mutational status

Bombonato¹,

Ribero

Pozzobon

et al. 2016

Acad Dermatol Venereol

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Association between dermoscopic and reflectance confocal microscopy features of cutaneous melanoma with BRAF mutational status

Bombonato¹,

Ribero

Pozzobon

et al. 2016

Acad Dermatol Venereol

Self Cite

View full text Add to dashboard Cite

show abstract

“…In particular, melanomas with common genetic mutations might share certain morphological features detectable with the noninvasive diagnostic tools used widely in common clinical practice in selected centers, such as dermoscopy and reflectance confocal microscopy (RCM). Recently published scientific articles have reported the analysis of the main dermoscopic differences in melanocytic lesions with certain genetic mutations in some research centers [6][7][8], but the use of RCM has not been reported before. In particular, Pozzobon et al [7] found that 'peppering', the dermoscopic pattern expressing regression and the presence of melanophages in the dermis, is the most common dermoscopic pattern in BRAF mutated melanomas compared with wild-type melanocytic cancer.…”

Section: Introductionmentioning

confidence: 99%

“…Recently published scientific articles have reported the analysis of the main dermoscopic differences in melanocytic lesions with certain genetic mutations in some research centers [6][7][8], but the use of RCM has not been reported before. In particular, Pozzobon et al [7] found that 'peppering', the dermoscopic pattern expressing regression and the presence of melanophages in the dermis, is the most common dermoscopic pattern in BRAF mutated melanomas compared with wild-type melanocytic cancer. We present our preliminary personal experience in the evaluation of BRAFV600E mutated melanomas with RCM compared with wild-type melanomas matched for age, sex, and thickness.…”

Section: Introductionmentioning

confidence: 99%

Confocal microscopy characterization of BRAFV600E mutated melanomas

Ruini

Manfredini²,

Pellacani³

et al. 2015

Melanoma Research

View full text Add to dashboard Cite

Thanks to modern techniques, molecular signatures for melanoma are now identifiable and have opened new horizons in the treatment of metastatic disease with molecular-targeted therapies. We distinguish different melanoma subtypes on the basis of genetic mutations such as BRAFV600E and we can therefore hypothesize the existence of corresponding morphological patterns that might be detected in vivo by noninvasive diagnostic tools such as dermoscopy and confocal microscopy. Eight BRAFV600E mutated melanomas (six primary and two metastases) were collected, matched in terms of age, sex, and thickness wild-type controls, and analyzed. In this preliminary study, regression, corresponding to fibrosis and melanophages in the dermis, was the predominant pattern and was also observed confocally when dermoscopy showed no peppering. In particular, confocal microscopy could not only detect regression but also provided a semiquantitative analysis of its grade through the count of melanophages. Confocal microscopy can be proposed as a useful tool in the preliminary screening and characterization of BRAFV600E mutated melanomas, providing new insights for patients' screening and follow-up.

show abstract

“…It has been shown that approximately 50% of melanomas have a BRAF V600 mutation [3]. Few recent studies have sought to correlate BRAF mutational status with features observed via non-invasive evaluation techniques, such as dermoscopy [4,5,6]. Researches have noted that BRAF mutated melanomas tend to be of the superficial spreading type and often reveal peppering on dermoscopy [5].…”

Section: Introductionmentioning

confidence: 99%

“…Few recent studies have sought to correlate BRAF mutational status with features observed via non-invasive evaluation techniques, such as dermoscopy [4,5,6]. Researches have noted that BRAF mutated melanomas tend to be of the superficial spreading type and often reveal peppering on dermoscopy [5]. Ruini et al used Reflectance Confocal Microscopy (RCM) to evaluate 8 melanomas and suggested that RCM may provide more specific information on the cytoarchitectural structure of BRAF mutated melanomas [7].…”

Section: Introductionmentioning

confidence: 99%

Reflectance confocal microscopy features of BRAF V600E mutated thin melanomas detected by immunohistochemistry

Urvanegia

Braga

Shitara³

et al. 2017

PLoS ONE

View full text Add to dashboard Cite

The classification of melanoma into four histological subtypes has been questioned regarding its clinical validity in providing relevant information for treatment for metastatic tumors. Specific genetic alterations are associated with particular clinical and histopathological features, suggesting that these could be helpful in refining existing melanoma classification schemes. We analyzed BRAF V600E mutated melanomas to explore the Reflectance confocal microscopy (RCM) utility as a screening aid in the evaluation of the most appropriate patients for genetic testing. Thus, 32 melanomas were assessed regarding their BRAF V600E mutational status. Experts blinded to dermoscopic images and V600E immunohistochemistry results evaluated RCM images regarding previously described melanoma features. BRAF positive melanomas were related to younger age (p = 0.035), invasive melanomas (p = 0.03) and to the presence of hiporreflective cells (p = 0.02), epidermal nests (p = 0.02), dermal-epidermal junction nests (p = 0.05), edged papillae (p = 0.05), and bright dots (p = 0.05), and to absence of junctional thickening due to isolated cells (p = 0.01) and meshwork (p = 0.02). This study can not characterize other mutations in the BRAF, because the immunohistochemistry is specific to the type V600E. The findings should encourage the genetic evaluation of BRAF mutation. This study highlights the potential of RCM as a supplementary tool in the screening of BRAF-mutated melanomas.

show abstract

Dermoscopic criteria associated with BRAF and NRAS mutation status in primary cutaneous melanoma

Abstract: This study showed a correlation between BRAF and NRAS status and dermoscopic findings of 'peppering' as an expression of regression and melanophages in the dermis, suggesting a morphological consequence of immune behaviour in BRAF-mutated melanomas.

Cited by 27 publications

References 14 publications

Association between dermoscopic and reflectance confocal microscopy features of cutaneous melanoma with BRAF mutational status

Association between dermoscopic and reflectance confocal microscopy features of cutaneous melanoma with BRAF mutational status

Confocal microscopy characterization of BRAFV600E mutated melanomas

Reflectance confocal microscopy features of BRAF V600E mutated thin melanomas detected by immunohistochemistry

Contact Info

Product

Resources

About