Descemet stripping automated endothelial keratoplasty with the EndoGlide Ultrathin graft insertion device

Ang, Andrea Y.; Liu, Yu-Chi; Tan, Donald

doi:10.1586/17434440.2014.947273

Cited by 9 publications

(6 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[10][11][12] In the first year following Descemet's membrane endothelial keratoplasty, Descemet's stripping automated endothelial keratoplasty, and penetrating keratoplasty, endothelial cell losses have been reported as 35%, 11 14.9%, 12 and 16.5%, 10 respectively. By the third postoperative year, endothelial cell losses will have increased to approximately 47%, 11 29.7%, 13,14 and 55%, 10 respectively. The progression of endothelial cell loss may eventually lead to graft failure and repeat graft surgeries in some patients.…”

mentioning

confidence: 99%

Predicative Factors for Corneal Endothelial Cell Migration

Soh

Peh

George

et al. 2016

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

PURPOSE.To characterize the effects of Descemet's stripping, Rho-associated protein kinase inhibitor Y-27632, and donor age on endothelial migration in human corneas maintained in ex vivo culture.METHODS. Twenty-eight cadaveric human corneas underwent ex vivo culture in either standard or Y-27632-supplemented culture medium for 14 days. The posterior surface of each cornea was manipulated to create two types of wounds: scratched wound-corneal endothelial cells (CECs) were denuded from the Descemet's membrane (DM) to leave behind a bare but intact DM; and peeled wound-both the DM and overlying CECs were stripped to leave behind bare corneal stroma. Endothelial migration was assessed via Trypan blue staining. Morphologic traits of CECs were assessed via Alizarin red microscopy and scanning electron microscopy.RESULTS. The CECs migrated preferentially over scratched wounds compared with peeled wounds. Y-27632 supplementation accelerated endothelial migration over scratched wounds. Endothelial migration decreased with advanced donor age for both wound types, regardless of exposure to Y-27632. Y-27632 supplementation resulted in a less rapid decline in endothelial migration for donors older than 50 years of age for scratched surfaces. Greater cell density and hexagonality was observed over scratched wounds compared with peeled wounds, regardless of Y-27632 supplementation.CONCLUSIONS. The presence of an intact DM, Y-27632 supplementation, and young donor age are factors that promote endothelial migration in an ex vivo human cornea culture model. The negative effect of age on endothelial migration can be mitigated by the presence of an intact DM and Y-27632 supplementation.

show abstract

mentioning

confidence: 99%

Predicative Factors for Corneal Endothelial Cell Migration

Soh

Peh

George

et al. 2016

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

show abstract

“…Graft rejection and graft failure are less frequent in newer methods of DSAEK such as ultra-thin DSAEK or in DSAEK using donor insertion device (15)(16)(17). The results of this study can be used to compare different transplantation techniques, including traditional PK, and more importantly, newer endothelial keratoplasty methods such as DMEK and DMET.…”

Section: Discussionmentioning

confidence: 91%

Visual Outcome, Refractive Error and Specular Microscopy Parameters Following Successful DSAEK

et al. 2018

View full text Add to dashboard Cite

Background: Descemet stripping and automated endothelial keratoplasty (DSAEK) has gained widespread popularity in the treatment of corneal endothelial dysfunction. In the present study, we evaluated the outcome of DSAEK in our center. Methods: This retrospective cross-sectional study was conducted on 60 patients, who had undergone DSAEK at least 12 months before in Khalili Hospital, Shiraz University of Medical Sciences. On follow-up, best spectacle-corrected visual acuity (BSCVA), refractive errors and specular microscopy parameters were assessed. Results: The majority of the studied eyes (49.2%) had a BSCVA within the range of 20/40 to 20/30. BSCVA was worse than 20/200 in 8 (13.3%) patients; 6 (75%) patients showed graft failure and 2 (25%) patients had graft rejection. Mean post-operative astigmatism was 0.29 ± 1.88 diopters. The mean refractive hyperopic shift was 1.30 ± 1.34 diopters. Specular microscopy indicated that 3 (5.1%) patients had cell density of less than 700 cells/mm 2 , but in none of the patients cell density was less than 500 cells/mm 2. Furthermore, hexagonality of endothelial cells in 50% of the eyes was 54% to 67%. Mean coefficient of variant was 27.76%. Abnormal variations in specular microscopy findings were mostly confined to endothelial cell density, sparing other parameters. Conclusions: In general, results of this study demonstrated acceptable BSCVA in the average duration of one-year post-DSAEK regarding late complications such as graft failure, graft rejection and epithelial downgrowth.

show abstract

“…Or they develop new technologies to solve problems that they have in the clinic. 3,4 Finally, the current research environment has required clinician scientists to develop the leadership skills to manage the complex healthcare system of the future. When I started out studying the genetics of human disease, we were carrying out what we thought were large studies involving a few hundred patients.…”

Section: What Does This Have To Do With Clinician Scientists?mentioning

confidence: 99%

Clinician Scientists in Public Sector Hospitals—Why and How?

2014

Ann Acad Med Singap

View full text Add to dashboard Cite

Descemet stripping automated endothelial keratoplasty with the EndoGlide Ultrathin graft insertion device

Cited by 9 publications

References 23 publications

Predicative Factors for Corneal Endothelial Cell Migration

Predicative Factors for Corneal Endothelial Cell Migration

Visual Outcome, Refractive Error and Specular Microscopy Parameters Following Successful DSAEK

Clinician Scientists in Public Sector Hospitals—Why and How?

Contact Info

Product

Resources

About