Describing <i>Paenibacillus mucilaginosus</i> strain N3 as an efficient plant growth promoting rhizobacteria (PGPR)

Goswami, Dweipayan; Parmar, Swapnsinh; Vaghela, Hemendrasinh; Dhandhukia, Pinakin; Thakker, Janki N.

doi:10.1080/23311932.2014.1000714

Cited by 65 publications

(34 citation statements)

References 42 publications

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“…Indeed, in addition to producing bioflocculants or plant hormones, this species is able to solubilize phosphates, to fix nitrogen and to produce ammonia. Moreover, it is able to produce siderophores, which contributes to plant growth by indirectly preventing the growth of plant pathogens [39,41]. Similarly, we could hypothesize that bacillopaline could contribute in the same way to maintain plant homeostasis.…”

Section: Resultsmentioning

confidence: 99%

Simple rules govern the diversity of bacterial nicotianamine-like metallophores

Laffont

Brutesco

Hajjar

et al. 2019

Preprint

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3In metal-scarce environments, some pathogenic bacteria produce opine-type metallophores 1 4 mainly to face the host's nutritional immunity. This is the case of staphylopine, pseudopaline and 1 5 yersinopine, identified in Staphylococcus aureus, Pseudomonas aeruginosa and Yersinia pestis 1 6respectively. These metallophores are synthesized by two (CntLM) or three enzymes (CntKLM), 1 7 CntM catalyzing the last step of biosynthesis using diverse substrates (pyruvate or α -ketoglutarate), 1 8 pathway intermediates (xNA or yNA) and cofactors (NADH or NADPH), depending on the species. 1 9Here, we explored substrate specificity of CntM by combining bioinformatics and structural analysis 2 0 with chemical synthesis and enzymatic studies. We found that NAD(P)H selectivity was mainly due to 2 1 the amino acid at position 33 (S. aureus numbering) which ensures a preferential binding to NADPH 2 2 when it is an arginine. Moreover, whereas CntM from P. aeruginosa preferentially uses yNA over 2 3 xNA, the staphylococcal enzyme is not stereospecific. Most importantly, selectivity towards α -2 4 ketoacids is largely governed by a single residue at position 150 of CntM (S. aureus numbering): an 2 5 aspartate at this position ensures selectivity towards pyruvate whereas an alanine leads to the 2 6 consumption of both pyruvate and α -ketoglutarate. Modifying this residue in P. aeruginosa led to a 2 7complete reversal of selectivity. Thus, opine-type metallophore diversity is mainly mediated by the 2 8absence/presence of a cntK gene encoding a histidine racemase, and the presence of an 2 9 aspartate/alanine at position 150 of CntM. These two simple rules predict the production of a fourth 3 0 metallophore by Paenibacillus mucilaginosus, which was confirmed in vitro and called bacillopaline. 3 1 3 2 3 3 3 5metals required for their growth. This is particularly the case for pathogenic bacteria that have to 3 6 confront the host's immune system. Indeed, the so-called "nutritional immunity" induces an additional 3 7 metal limitation by sequestering iron, zinc or manganese to prevent bacterial growth [1-4]. To face 3 8 this metal restriction, bacteria have developed metallophores to recover metals. In this context, 3 9 nicotianamine-like metallophores have been identified in some bacteria as playing an important role in 4 0 metal acquisition strategies. Staphylopine, pseudopaline and yersinopine are the three examples 4 1 2 currently known and recently identified in S. aureus [5], P. aeruginosa [6,7] and Y. pestis [8] 4 2respectively. The biosynthesis of these nicotianamine-like metallophores occurs in two or three steps 4 3 depending on the species. When it is present, as in S. aureus, CntK, a histidine racemase, transforms 4 4

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Section: Resultsmentioning

confidence: 99%

Simple rules govern the diversity of bacterial nicotianamine-like metallophores

Laffont

Brutesco

Hajjar

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…In previous studies, the Bacillus subtilis and Paenibacillus spp. have been well documented as Gram positive, rod shaped, plant growth promoting rhizobacteria with catalase activity, phosphate solubilization activity, as well as other traits (Govindasamy et al, 2010;Goswami et al, 2015). Nitrogen fixation abilities of Paenibacillus have even been reported (Xie et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Peer Review #1 of "Preliminary study on phosphate solubilizing Bacillus subtilis strain Q3 and Paenibacillus sp. strain Q6 for improving cotton growth under alkaline conditions (v0.1)"

Boon¹

2018

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Background: Low phosphorus availability limits crop production in alkaline calcareous soils in semi-arid regions including Pakistan. Phosphate solubilizing bacteria may improve crop growth on alkaline calcareous soils due to their ability to enhance P availability. Methods: Twenty rhizobacterial isolates (Q1-Q20) were isolated from rhizosphere of cotton and characterized for their growth promoting attributes in vitro. The selected phosphate solubilizing isolates were further screened for their ability to improve cotton growth under axenic conditions (jar trial). The phosphorus solubilization capacities of selected strains were quantified and these strains were identified through 16S rDNA sequencing. Results: Isolates Q2, Q3, Q6, Q7, Q8, Q13 and Q14 were able to solubilize phosphate from insoluble sources. Most of these isolates also possessed other traits including catalase activity and ammonia production. The growth promotion assay showed that Q3 was significantly better than most of the other isolates followed by Q6. Maximum root colonization (4.34 × 10 6 cfu g-1) was observed in case of isolate Q6 followed by Q3. The phosphorus solubilization capacities of these strains were quantified, showing a maximum phosphorus solubilization by Q3 (Optical density 2.605 ± 0.06) followed by the Q6 strain. The strain Q3 was identified as Bacillus subtilis (Accession # KX788864) and Q6 as Paenibacillus sp. (Accession # KX788865) through 16S rDNA sequencing. Discussion: The bacterial isolates varied in their abilities for different growth promoting traits. The selected PGPR Bacillus subtilis strain Q3 and Paenibacillus sp. strain Q6 have multifarious growth promoting traits including ability to grow at higher EC and pH levels, and phosphorus solubilizing ability. These strains can efficiently colonize cotton roots under salt affected soils and help plants in phosphorus nutrition. It is concluded that both strains are potential

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“…The nitrogen-fixing ability of three bacterial isolates was confirmed by growing the isolates on semi-solid nitrogen-free bromothymol blue (NFB) medium ( Goswami et al, 2015 ). A total of 50 μL of overnight bacterial culture in YEP broth was pipetted onto the Petri plate with NFB medium and incubated in the dark at 28°C for 4 days.…”

Section: Methodsmentioning

confidence: 99%

Identification of Soil Bacterial Isolates Suppressing Different Phytophthora spp. and Promoting Plant Growth

et al. 2018

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Bacterial isolates obtained from the rhizosphere of Arabidopsis and a plantless compost potting mix was screened for anti-oomycete activity against Phytophthora capsici, Phytophthora citricola, Phytophthora palmivora, and Phytophthora cinnamomi. Three out of 48 isolates exhibited more than 65% inhibition against all tested Phytophthora species and were selected for further studies. These strains, named UQ154, UQ156, and UQ202, are closely related to Bacillus amyloliquefaciens, Bacillus velezensis, and Acinetobacter sp., respectively, based on 16S rDNA sequence analysis. The isolates were evaluated for their ability to fix nitrogen, solubilize phosphate, as well as for siderophore, indoleacetic acid, cell wall degrading enzymes and biofilm production. Their plant growth promoting activities were evaluated by measuring their effect on the germination percentage, root and shoot length, and seedling vigor of lettuce plants. All of these traits were significantly enhanced in plants grown from seeds inoculated with the isolates compared with control plants. Moreover, bacteria-inoculated P. capsici-infected chili plants exhibited improved productivity based on CO2 assimilation rates. Both real-time quantitative PCR and disease severity index revealed significant decreases in pathogen load in infected chili root tissues when plants were previously inoculated with the isolates. Biocontrol activity may result from the secretion of diketopiperazines as identified by Gas chromatography-mass spectrometry analysis of bacterial cultures’ extracts. Collectively, this work demonstrates the potential of bacterial isolates to control Phytophthora infection and promote plant growth. They can, therefore be considered as candidate microbial biofertilizers and biopesticides.

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Describing Paenibacillus mucilaginosus strain N3 as an efficient plant growth promoting rhizobacteria (PGPR)

Cited by 65 publications

References 42 publications

Simple rules govern the diversity of bacterial nicotianamine-like metallophores

Simple rules govern the diversity of bacterial nicotianamine-like metallophores

Peer Review #1 of "Preliminary study on phosphate solubilizing Bacillus subtilis strain Q3 and Paenibacillus sp. strain Q6 for improving cotton growth under alkaline conditions (v0.1)"

Identification of Soil Bacterial Isolates Suppressing Different Phytophthora spp. and Promoting Plant Growth

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