Describing the role of Drosophila melanogaster ABC transporters in insecticide biology using CRISPR-Cas9 knockouts

Denecke, Shane; Fusetto, Roberto; Batterham, Philip

doi:10.1016/j.ibmb.2017.09.017

Cited by 52 publications

(52 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The downregulated DEGs were also enriched in ABC transporters. As the largest family of active transporters, previous research on ABC transporters has mostly focused on pesticide sensitivity and metabolism [56,57], but the results of this study indicate that these transporters might also participate in the cellular immune process. All three pathways were inhibited in the DENV-2-infected Aag2 cells, which suggests that the genes related to these pathways might be involved in the host-virus interaction process and that enhancing the expression of these genes might inhibit or even block virus transmission.…”

Section: Analysis Of Other Pathwaysmentioning

confidence: 81%

Transcriptome analysis of Aedes aegypti Aag2 cells in response to dengue virus-2 infection

Ce-jie

Gao

et al. 2020

Parasites Vectors

View full text Add to dashboard Cite

Background: Dengue virus (DENV) is a flavivirus transmitted by mosquitoes that is prevalent in tropical and subtropical countries and has four serotypes (DENV1-4). Aedes aegypti, as the main transmission vector of DENV, exhibits strong infectivity and transmission. With the aim of obtaining a better understanding of the Ae. aegypti-DENV interaction, the transcriptome changes in DENV-2-infected Aag2 cells were studied to describe the immune responses of mosquitoes using the Ae. aegypti Aag2 cell line as a model. Methods: RNAseq technology was used to sequence the transcripts of the Ae. aegypti Aag2 cell line before and after infection with DENV-2. A bioinformatics analysis was then performed to assess the biological functions of the differentially expressed genes, and the sequencing data were verified by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Results: The transcriptome analysis generated 8866 unigenes that were found in both groups, 225 unigenes that were only found in the infection group, and 683 unigenes that only existed in the control group. A total of 1199 differentially expressed genes, including 1014 upregulated and 185 downregulated genes, were identified. The bioinformatics analysis showed that the differentially expressed genes were mainly involved in the longevity regulating pathway, circadian rhythm, DNA replication, and peroxisome, purine, pyrimidine, and drug metabolism. The qRT-PCR verification results showed the same trend, which confirmed that the expression of the differentially expressed genes had changed, and that the transcriptome sequencing data were reliable. Conclusions: This study investigated the changes in the transcriptome levels in the DENV-2-infected Ae. aegypti Aag2 cell line, which provides a faster and effective method for discovering genes related to Ae. aegypti pathogen susceptibility. The findings provide basic data and directions for further research on the complex mechanism underlying host-pathogen interactions.

show abstract

Section: Analysis Of Other Pathwaysmentioning

confidence: 81%

Transcriptome analysis of Aedes aegypti Aag2 cells in response to dengue virus-2 infection

Ce-jie

Gao

et al. 2020

Parasites Vectors

View full text Add to dashboard Cite

show abstract

“…D. melanogaster with a Mdr65 -gene knockout via CRISPR/cas9 had increased susceptibility to all neuroactive insecticides tested. Deficiency crosses, synergism with the ABC inhibitor verapamil and accumulation of pesticides in the Mdr65 -knockout individuals further confirmed that Mdr65 plays an important role in pesticide transport [105]. Tissue-specific RNAi of Mdr65 also confirmed the role of the gene in reduced toxicity of multiple insecticides in D. melanogaster [106].…”

Section: Abc Transporters In Xenobiotic Detoxification By Insectsmentioning

confidence: 90%

Insect ATP-Binding Cassette (ABC) Transporters: Roles in Xenobiotic Detoxification and Bt Insecticidal Activity

Chakrabarty

Jin

et al. 2019

IJMS

117

View full text Add to dashboard Cite

ATP-binding cassette (ABC) transporters, a large class of transmembrane proteins, are widely found in organisms and play an important role in the transport of xenobiotics. Insect ABC transporters are involved in insecticide detoxification and Bacillus thuringiensis (Bt) toxin perforation. The complete ABC transporter is composed of two hydrophobic transmembrane domains (TMDs) and two nucleotide binding domains (NBDs). Conformational changes that are needed for their action are mediated by ATP hydrolysis. According to the similarity among their sequences and organization of conserved ATP-binding cassette domains, insect ABC transporters have been divided into eight subfamilies (ABCA–ABCH). This review describes the functions and mechanisms of ABC transporters in insecticide detoxification, plant toxic secondary metabolites transport and insecticidal activity of Bt toxin. With improved understanding of the role and mechanisms of ABC transporter in resistance to insecticides and Bt toxins, we can identify valuable target sites for developing new strategies to control pests and manage resistance and achieve green pest control.

show abstract

“…Also, is important to highlight the presence of ABC transporters, which are proteins of great interest for biotechnological applications, in our work expression of predicted ABC transporters was higher in insects fed with sugarcane. Genes from this family have been reported to play a role in the development of resistance to insecticides and even adaptation to plant defences, mainly by eliminating molecules through the gut membranes [55,56]. Furthermore, some ABC transporters act as receptors for Bt toxins [57,58].…”

Section: Molecular Transportmentioning

confidence: 99%

Comparative gut transcriptome analysis of Diatraea saccharalis in response to the dietary source

et al. 2020

View full text Add to dashboard Cite

The sugarcane borer (Diatraea saccharalis, Fabricius, 1794) is a devastating pest that causes millions of dollars of losses each year to sugarcane producers by reducing sugar and ethanol yields. The control of this pest is difficult due to its endophytic behavior and rapid development. Pest management through biotechnological approaches has emerged in recent years as an alternative to currently applied methods. Genetic information about the target pests is often required to perform biotechnology-based management. The genomic and transcriptomic data for D. saccharalis are very limited. Herein, we report a tissue-specific transcriptome of D. saccharalis larvae and a differential expression analysis highlighting the physiological characteristics of this pest in response to two different diets: sugarcane and an artificial diet. Sequencing was performed on the Illumina HiSeq 2000 platform, and a de novo assembly was generated. A total of 27,626 protein-coding unigenes were identified, among which 1,934 sequences were differentially expressed between treatments. Processes such as defence, digestion, detoxification, signaling, and transport were highly represented among the differentially expressed genes (DEGs). Furthermore, seven aminopeptidase genes were identified as candidates to encode receptors of Cry proteins, which are toxins of Bacillus thuringiensis used to control lepidopteran pests. Since plantinsect interactions have produced a considerable number of adaptive responses in hosts and herbivorous insects, the success of phytophagous insects relies on their ability to overcome challenges such as the response to plant defences and the intake of nutrients. In this study, we identified metabolic pathways and specific genes involved in these processes. Thus, our data strongly contribute to the knowledge advancement of insect transcripts, which can be a source of target genes for pest management.

show abstract

Describing the role of Drosophila melanogaster ABC transporters in insecticide biology using CRISPR-Cas9 knockouts

Cited by 52 publications

References 44 publications

Transcriptome analysis of Aedes aegypti Aag2 cells in response to dengue virus-2 infection

Transcriptome analysis of Aedes aegypti Aag2 cells in response to dengue virus-2 infection

Insect ATP-Binding Cassette (ABC) Transporters: Roles in Xenobiotic Detoxification and Bt Insecticidal Activity

Comparative gut transcriptome analysis of Diatraea saccharalis in response to the dietary source

Contact Info

Product

Resources

About