Descripción Histológica Del Complejo Folicular Piloso en Crías De Alpacas

Background: Alpacas are reared mainly for fiber extraction, which is a highly valued product in the textile industry. For this reason, this work aims to evaluate the morphological and quantitative aspects of the light and dark alpaca skin of Huacaya and Suri alpacas, comparing the structure and architecture of the scapular, costal and lateral femoral skin. Biopsies were collected from the skin of 12 alpacas from the Pacomarca Experimental Fund, located at Puno - Peru. The samples were weighed and fixed in 10% aqueous formalin solution for histological procedures. The histological sections were stained with Hematoxylin eosin, Picrossirius red and Masson Trichromic and immunostained for types I, III and IV collagen and S100.Results: The derma presented sebaceous and sweat glands, as well as follicular groups with primary and secondary hair follicles. Each follicle had a hair called fiber, some with medulla and some without, but both surrounded by cortex and cuticle. The skin presented similar immunostaining for type I, II and IV and S100. Collagen III was detected only in the derma. The total volume of the skin, derma, follicular groups and sebaceous and sweat glands was estimated by stereology for the three body regions for both Huacaya and Suri alpacas. The total volume of skin (Vref) and total volume of follicular groups (VGF) were different for body regions. Femoral region showed higher values for VGF. Colour and breed were also different for Vref and total volume of derma (VD).Conclusion: Comparing the two breeds the femoral region presented higher fiber production. Dark animals had more derma and it was reported close relationship between total skin volume and their fractions volumes: derma, follicular groups and sweat glands.

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“…Authors found one to three primary follicles within a follicular group, and between 20 and 33 secondary follicles [3,[31][32][33].…”

Section: Discussionmentioning

confidence: 99%

The architecture and structure of alpaca skin (Vicugna pacos): impacts on industry and wool trade

Sasahara

McManus

Neira

et al. 2020

Preprint

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“…In order to obtain the bestquality and largest digital images, 10 samples from Huacaya and 11 from Suri were selected for histological sections. The level immediately below the sebaceous gland was defined as the most suitable depth containing with the maximum number of detectable primary follicles for microscope observations, thereby increasing the possibility of identifying the follicular groups (McCloghry et al, 1997a;Antonini, 2010;García and Pezo, 2012).…”

Section: Laboratory Analysismentioning

confidence: 99%

Spatial structure of skin follicles in Suri and Huacaya alpacas

Molina

Teich

Antonini

et al. 2016

Small Ruminant Research

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“…No presente estudo encontrou-se folículos pilosos tanto dentro, quanto fora dos grupos foliculares. Outros autores como (ANTONINI; GONZALES; VALBONESI, 2004;BADAJOZ et al, 2009;ANTONINI, 2010;MOLINA et al, 2016), acharam de um até três folículos primários dentro de um grupo folicular, juntamente com uma quantidade de 20 até 33 folículos secundários. O colágeno é uma proteína de matriz, a qual confere maior rigidez e consistência à pele, fazendo com que as estruturas da derme tais como os folículos pilosos, glândulas, nervos, vasos sanguíneos e músculos eretores do pelo, permaneçam estáveis dentro do tecido dérmico, servindo de base para a sustentação e nutrição do pelo como descrito por (ATLEE et al, 1997;SAMUELSON, 2007;LACOLLA et al, 2010;MERUANE;ROJAS, 2012).…”

Section: Discussionunclassified

“…Já se sabe que para os camelídeos segundo (ATLEE et al, 1997;ANTONINI;GONZALES;VALBONESI, 2004;BADAJOZ et al, 2009;VÉLEZ et al, 2009VÉLEZ et al, , 2016ANTONINI, 2010;LACOLLA et al, 2010;MALONEY, 2011; FERGUSON; MCGREGOR; BEHRENDT, 2012), os grupos foliculares apresentam mais de um folículo primário e vários folículos secundários. Conforme os pelos emergem vão se juntando para compartilhar um mesmo folículo.…”

Section: Discussionunclassified

“…Os pelos, são estruturas filamentosas, formadas por células epiteliais queratinizadas, que se desenvolvem a partir de uma invaginação da epiderme que invade a derme, chamada de folículo piloso. Este constitui o melhor isolamento térmico da pele e confere sua resistência à agua e encontra-se orientado obliquamente (GETTY, 1986;BANKS, 1991;SAMUELSON, 2007 BUSTINZA, 1985;BANKS, 1991;ATLEE et al, 1997;BUSTINZA, 2001;ANTONINI;GONZALES;VALBONESI, 2004;VÉLEZ et al, 2009;BADAJOZ et al, 2009;ANTONINI, 2010;LACOLLA et al, MONTES et al, 2008;CERVANTES et al, 2009;GUTIÉRREZ et al, 2009;ORIA et al, 2009;QUISPE et al, 2009 Legenda: Em A, observamos a tricotomia das três regiões analisadas. Em B, a assepsia da pele para realizar a infiltração de lidocaína 2% como método de anestesia local em C. Em D, a biopsia, feita na região costal com um punch de 8 mm.…”

Section: Pelosunclassified

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>b<Arquitetura e estrutura da pelagem da Alpaca(>i<Vicugna pacos>/i<)>/b<

Neira¹

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Alpacas are mainly raised for fiber production, which is a highly valued product in the textile industry. Therefore the purpose of this project aims to evaluate morphological and quantitative aspects of alpaca´s coat in Huacaya and Suri breeds, comparing the structure and architecture of the skin forelimb lateral region, thoracic lateral region, and hind limb lateral region, with three sub-regions: scapular, costal and lateral femoral respectively. For this project, 8 mm biopsies were collected from the coat of 12 alpacas, from the Pacomarca Experimental Fund, located in the city of Puno-Peru. The samples were weighed, immersed in 10% aqueous formaldehyde solution and then processed. After that, they were cut into fragments of 2 mm each, in order to obtain random samples for Stereological analyzes. Hematoxylin and Eosin, Picrosirius Red and Masson's Trichrome stains were used for the microscopic analysis. This last staining was also required for Stereological analysis. After obtaining the images, a slide with the points system was added to each sample in order to count the structures to be evaluated. Subsequently, markers for type I, III and IV collagen proteins were used to perform immunohistochemical analysis, as well as for S100. The structures found in the integumentary system of alpacas were the components of the skin, such as the epidermis, the dermis, and separating the two structures, the basal lamina. In the dermis it was possible to observed the sebaceous glands and sweat glands, as well as the follicular groups formed by primary follicles and secondary hair follicles in abundance. From each of these follicles a hair emerged called fiber. One of the factors used for this evaluation was the observation of the fibers diameter, which was measured in samples obtained from the costal region of the alpacas, and carried out by means of scanning electron microscopy technique. Measurements of 12.65 μm up to 55.00 μm were obtained. It was possible to observed fibers that had marrow and others that did not have marrow, making it possible to differ them in the three layers that composed the hair. Together, with the marrow, they composed the cortex and the cuticle, in addition to the layers of the hair follicle. Extracellular matrix proteins were also evaluated, in which type III collagen fibers were found, which marked only the collagen and reticular fibers present in the dermis and presenting a different marking when compared to the other collagens including S100 protein. The volume of the skin, dermis, follicular groups, sebaceous glands and sweat glands were evaluated through Stereological analysis, among the three body regions of each group of alpacas at a significance level of p <0.05, resulting in differences in the mean volume of the dermis and the mean volume of follicular groups in dark-colored Suri alpacas. The four groups of alpacas for each body region were also compared, with a significance level of p<0.05, and p>0.05 for the mean volumes of skin and dermis in the lateral femoral region.

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Descripción Histológica Del Complejo Folicular Piloso en Crías De Alpacas

Cited by 4 publications

References 3 publications

The architecture and structure of alpaca skin (Vicugna pacos): impacts on industry and wool trade

The architecture and structure of alpaca skin (Vicugna pacos): impacts on industry and wool trade

Spatial structure of skin follicles in Suri and Huacaya alpacas

>b<Arquitetura e estrutura da pelagem da Alpaca(>i<Vicugna pacos>/i<)>/b<

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