Description and genetic characterisation of Hysterothylacium (Nematoda: Raphidascarididae) larvae parasitic in Australian marine fishes

Shamsi, Shokoofeh; Gasser, Robin B.; Beveridge, Ian

doi:10.1016/j.parint.2012.10.001

Cited by 61 publications

(80 citation statements)

References 15 publications

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“…However, only 2 previous studies have reported genetic analyses of this genus from the Brazilian coast (Knoff et al, 2012;Borges et al, 2012). According to Shamsi et al (2013), the identification of the larval stages of this genus to the generic level using only morphological characters is difficult because there are several other genera having an intestinal caecum, ventricular appendix and an excretory pore at the level of the nerve ring, e.g., Paraheterotyphlum, Heterotyphlum, Iheringascaris and Lappetascaris. In the present study, the excretory pore was visible near the nerve ring, which also distinguishes it from Contracaecum (L3) (Moravec, 1998), and a molecular phylogenetic analysis showed the gene sequence similarity of the larvae collected for this study to the sequences of genus Hysterothylacium deposited in GenBank.…”

Section: Hysterothylacium Sp (L3) -Measurements Of Larvae Based On 2mentioning

confidence: 99%

Molecular and Morphological Characterization of Anisakid Nematode Larvae from the SandperchesPseudopercis numidaandPinguipes brasilianus(Perciformes: Pinguipedidae) off Brazil

Pantoja

Borges

Santos

et al. 2015

Journal of Parasitology

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Morphological and genetic data on the larvae of Anisakidae parasitizing the sandperches Pseudopercis numida and Pinguipes brasilianus were recorded from Cabo Frio, State of Rio de Janeiro, Brazil (23°S, 42°W). Twenty-five specimens of P. numida and 25 specimens of P. brasilianus were necropsied. The 352 larvae collected included 1 Anisakis sp., 344 Hysterothylacium sp., and 7 Raphidascaris sp. We present mtDNA sequence data for Anisakis sp. and Hysterothylacium sp., and we sequenced the ITS ribosomal DNA (rDNA) of Hysterothylacium sp. and Raphidascaris sp. The morphological and genetic profiles confirmed the identification of Anisakis typica (Diesing, 1860). Hysterothylacium sp. and Raphidascaris sp. were not identified to the species level due to the absence of similar sequences for adult parasites. The mtDNA sequence of Hysterothylacium sp. from P. numida showed a high similarity to the sequence of Hysterothylacium deardorffoverstreetorum. However, a phylogenetic analysis demonstrated the presence of different clades under the same name as the larval H. deardorffoverstreetorum. Pseudopercis numida is a new host record for A. typica.

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Section: Hysterothylacium Sp (L3) -Measurements Of Larvae Based On 2mentioning

confidence: 99%

Molecular and Morphological Characterization of Anisakid Nematode Larvae from the SandperchesPseudopercis numidaandPinguipes brasilianus(Perciformes: Pinguipedidae) off Brazil

Pantoja

Borges

Santos

et al. 2015

Journal of Parasitology

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“…We also concluded that genetic characterization based nuclear and mitochondrial gene regions with sufficient phylogenetic signal should be conducted to obtain the true identification of the same larval stages of Hysterothylacium species from similar geographical areas and to explore the species diversity. In accordance to this inference Shamsi et al [16] also indicated that, even in the genetic characterization based on the ITS-1 and ITS-2 sequences of the same larval stage, some morphotypes could contain different genotypes.…”

Section: H Fabri Was Identified From Phycis Phycis Alosa Fallax Comentioning

confidence: 79%

Türkiye’nin Ege Denizi’nden Yakalanan Kupes (Boops boops) Balıklarında Hysterothylacium Larvalarının İdentifikasyonu ve Moleküler Karakterizasyonu

Şimşek¹,

Çiloğlu²,

Yıldırım³

et al. 2018

Kafkas Univ Vet Fak Derg

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Hysterothylacium species are the most prevalent ascaridoid nematode reported from various freshwater, estuarial and marine fish species. However, there are very few studies about molecular identification and characterization of Hysterothylacium species in marine fish species from Turkish waters. The aim of the present study was to molecular identification and characterization of Hysterothylacium larvae from Boops boops caught off Aegean Sea, Turkey. Hysterothylacium larvae were found in 10 of 109 B. boops. The prevalence of Hysterothylacium spp. larvae was 9.2%. A total of 10 larvae of Hysterothylacium spp. were collected from all infected fish. Hysterothylacium larvae were genetically identified as H. aduncum, H. fabri and H. reliquens from Turkish waters by using sequence analyses of rDNA ITS regions in B. boops for the first time. In addition, B. boops was reported as a new host for H. reliquens. Moreover, H. reliquens was characterized for the first time by sequencing of the ITS regions from the Turkish waters with the present study. Keywords

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“…Recent reports in Italy suggest that A. pegreffii can be the etiological agent of human infection (Mattiucci et al 2013). Genus Hysterothylacium Ward and Magath, 1917 is one of the common genera in the family Raphidascarididae (Fageholm 1991), in which there are approximately 70 species (Shamsi et al 2013). The adult stages are usually found in the alimentary canal of predatory teleost, while the L3 are found encapsulated in the mesentery and viscera of various species of marine teleosts and also in various marine invertebrates (Keskin et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…The adult stages are usually found in the alimentary canal of predatory teleost, while the L3 are found encapsulated in the mesentery and viscera of various species of marine teleosts and also in various marine invertebrates (Keskin et al 2015). Thus, it is not host specific in the larval stages, exhibiting a global distribution (Shamsi et al 2013). Many species of the genus Hysterothylacium have been poorly described; thus, their genetic characteristics have been frequently deposited in Genbank without clear identification at the species level.…”

Section: Discussionmentioning

confidence: 99%

Genetic identification of anisakid nematodes isolated from largehead hairtail (Trichiurus japonicus) in Korea

2016

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Background: The nematode species belonging to genus Anisakis occur at their third larval stage in numerous marine teleost fish species worldwide and known to cause accidental human infection through the ingestion of raw or undercooked fish or squids. They may also draw the attention of consumers because of the visual impact of both alive and dead worms. Therefore, the information on their geographical distribution and clear species identification is important for epidemiological survey and further prevention of human infection. Results: For identification of anisakid nematodes species isolated from largehead hairtail (Trichiurus japonicus), polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of internal transcribed spacers of ribosomal DNA were conducted. Mitochondrial cytochrome c oxidase subunit 2 gene was also sequenced, and phylogenetic analysis was conducted. From the largehead hairtail (n = 9), 1259 nematodes were isolated in total. Most of the nematodes were found encapsulated throughout the viscera (56.2 %, 708/1259) or moving freely in the body cavity (41.5 %, 523/1259), and only 0.3 % (4/1259) was found in the muscles. By PCR-RFLP, three different nematode species were identified. Anisakis pegreffii was the most dominantly found (98. 7 %, 1243/1259) from the largehead hairtail, occupying 98.7 % (699/708) of the nematodes in the mesenteries and 98.1 % (513/523) in the body cavity. Hybrid genotype (Anisakis simplex × A. pegreffii) occupied 0.5 %, and Hysterothylacium sp. occupied 0.2 % of the nematodes isolated in this study. Conclusions:The largehead hairtail may not significantly contribute accidental human infection of anisakid nematode third stage larvae because most of the nematodes were found from the viscera or body cavity, which are not consumed raw. But, a high prevalence of anisakid nematode larvae in the largehead hairtail is still in concern because they may raise food safety problems to consumers. Immediate evisceration or freezing of fish after catch will be necessary before consumption.

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Description and genetic characterisation of Hysterothylacium (Nematoda: Raphidascarididae) larvae parasitic in Australian marine fishes

Cited by 61 publications

References 15 publications

Molecular and Morphological Characterization of Anisakid Nematode Larvae from the SandperchesPseudopercis numidaandPinguipes brasilianus(Perciformes: Pinguipedidae) off Brazil

Molecular and Morphological Characterization of Anisakid Nematode Larvae from the SandperchesPseudopercis numidaandPinguipes brasilianus(Perciformes: Pinguipedidae) off Brazil

Türkiye’nin Ege Denizi’nden Yakalanan Kupes (Boops boops) Balıklarında Hysterothylacium Larvalarının İdentifikasyonu ve Moleküler Karakterizasyonu

Genetic identification of anisakid nematodes isolated from largehead hairtail (Trichiurus japonicus) in Korea

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