Description and prevalence of Thynnascaris sp. larvae Dollfus, 1933 (Nematoda: Anisakidae) in Plagioscion squamosissimus Heckel, 1840 from Volta Grande Reservoir, State of Minas Gerais, Brazil

Martins, Maurício Laterça; Fujimoto, R. Y.; Moraes, Flávio Ruas de; Andrade, Pedro de; Nascimento, Adjair Antônio do; Malheiros, Euclides Braga

doi:10.1590/s0034-71082000000300017

Cited by 8 publications

(4 citation statements)

References 13 publications

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“…In this assay, neutrophill migration was more pronounced by saline and thioglycollate injections which were also corroborated by the findings of Do Vale et al (2002). Finally, these results might explain the migration of cells involved in the inflammatory response caused by parasites in native fish (Martins et al, 2000(Martins et al, , 2001b.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the acute inflammatory response in the hybrid tambacu (Piaractus mesopotamicus male × Colossoma macropomum female) (Osteichthyes)

Martins

Myiazaki²,

Tavares‐Dias

et al. 2009

Braz. J. Biol.

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This work evaluated the acute inflammatory response induced by injections of 0.5 mL saline solution (control), 500 µg carrageenin and 0.5 mL thioglycollate 3% in the swim bladder of juvenile tambacu hybrid. Fish were distributed in three treatments, three replications and acclimated for a period of 10 days before assay. The cell characterization from the inflammatory exudate was performed in Giemsa and PAS stained smears. Carrageenin, injected in fish, showed an increase on the total number of cells in the inflammatory exudate when compared to saline and thioglycollate injected. Whereas, for carrageenin-injected fish, the percentage of thrombocyte was higher than thioglycollate. On the other hand, granulocyte percentage in thioglycollate-injected fish was higher than the ones injected using carrageenin. Carrageenin provoked the highest migration of macrophage to the inflammatory site. The PAS method confirmed the presence of three types of granulocytes: eosinophilic granular cell (EGC) type 1 with the characteristics of a special granulocytic cell commonly found in the circulating blood; EGC type 2 shorter than the last one and neutrophil. This study contributes to a better understanding of the inflammatory response and infectious processes in native fish.Keywords: fish, hybrid tambacu, inflammation, exudate, carrageenin, thioglycollate. Caracterização da resposta inflamatória aguda no híbrido tambacu (Piaractus mesopotamicus macho × Colossoma macropomum fêmea) (Osteichthyes) ResumoEste estudo avaliou a resposta inflamatória aguda induzida por injeções de 0,5 mL de solução salina (controle), 500 µg de carragenina e 0,5 mL de tioglicolato a 3% na bexiga natatória de juvenis do híbrido tambacu. Os peixes foram distribuídos em três tratamentos, três repetições e aclimatados durante 10 dias antes do ensaio. A caracterização das células do exsudato inflamatório foi feita após coloração com Giemsa e PAS. Peixes injetados com carragenina apresentaram maior número de células no exsudato inflamatório do que com salina e tioglicolato. A porcentagem de trombócitos no exsudato foi maior nos injetados com carragenina quando comparada com a dos injetados com tioglicolato. Por outro lado, o percentual de granulócitos foi maior em animais injetados com tioglicolato do que em animais injetados com carragenina. A carragenina provocou maior migração de macrófagos para o foco inflamatório. O método de PAS Injection and collection of exudate cellsThe fish were anaesthetized by immersion in benzocaine solution (1 g.10 L -1 ) and injected with 500 µg carrageenin (Marine Colloids) dissolved in 0.5 mL sterile saline solution, 0.5 mL thioglycollate 3% and 0.5 mL sterile saline solution (control). After injection, the fish were maintained in the aquaria for 6 hours until the sacrifice. The interior of the swim bladder was washed with complete phosphate buffered saline containing 0.01 mL EDTA 5% and cell suspensions were centrifuged at 150 × gravity for 10 minutes before staining. The total number of cells was determined with a ha...

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Section: Discussionmentioning

confidence: 99%

Characterization of the acute inflammatory response in the hybrid tambacu (Piaractus mesopotamicus male × Colossoma macropomum female) (Osteichthyes)

Martins

Myiazaki²,

Tavares‐Dias

et al. 2009

Braz. J. Biol.

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“…Various studies have reported 23 parasitic species in the South American silver croaker, including one cestode (WOODLAND, 1935), two digenetic (THATCHER, 1979;KOHN et al, 1995), 12 monogeneans (KRITSKY & THATCHER, 1984;MARTINS et al, 2000a;KRITSKY & BOEGER, 2002;BOEGER & KRITSKY, 2009), two acanthocephalans (THATCHER, 1980MELO et al, 2015), four nematodes (MORAVEC et al, 1993;MARTINS et al, 2000b;TAVARES et al, 2007) and two copepod crustaceans (AMADO & ROCHA, 1996;THATCHER, 1998). Most of these studies were conducted in the Amazon and Paraná basins.…”

Section: Introductionmentioning

confidence: 99%

Metazoan parasites of Plagioscion squamosissimus, an invasive species in the Tietê River, São Paulo, Brazil

Lapera

Silva

Canônico

et al. 2017

Rev. Bras. Parasitol. Vet.

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This study focused on the characterization and analysis of communities and infra-communities of metazoan parasites of Plagioscion squamosissimus caught in Promissão Reservoir in the Tietê River in Borborema (21°39′58"S, 49°8′49"W), state of São Paulo, Brazil. Fifty adult specimens caught by professional fishermen in March 2015 were necropsied. The fish presented an average standard length of 25.2 ± 2.2 cm and average weight of 328.82 ± 89.03 g. A total of 5,227 specimens of metazoan parasites were collected: 2,880 (55.1%) adult Diplectanum piscinarius (Monogenoidea: Diplectanidae) and 2,347 (44.9%) Austrodiplostomum compactum metacercariae (Digenea, Diplostomidae), both with 100% prevalence and mean abundance of 57.6 and 46.9, respectively. Parasite diversity was low ( ResumoO objetivo deste trabalho foi caracterizar e analisar as comunidades e infracomunidades de metazoários parasitos de corvinas capturadas no Reservatório de Promissão, Rio Tietê, município de Borborema (21° 67′S, 49° 14′O), Estado de São Paulo. Foram examinados 50 espécimes, capturados por pescadores profissionais no mês de março de 2015, e os parasitas coletados foram quantificados, preparados e montados para identificação taxonômica e análise das comunidades de parasitos. Os peixes analisados no estudo apresentaram comprimento padrão médio de 25,2 ± 2,2 cm e peso médio de 328,82 ± 89,03 g. Foram coletados 5227 espécimes de parasitas metazoários, sendo 2880 (55,1%) Diplectanum piscinarius (Monogenoidea: Diplectanidae) e 2347 (44,9%) metacercárias de Austrodiplostomum compactum (Digenea, Diplostomidae), ambos com prevalência de 100% e abundância parasitária de 57,6 e 46,9, respectivamente. Foi encontrada baixa diversidade parasitária (riqueza de espécies=2), com índice de Simpson (D) igual a 0,505 e baixos valores dos índices de Shannon (H'=0,688) e de diversidade de Margalef (I=0,177). O índice de dominância de Berger-Parker (d=0,551) indicou uma leve dominância do monogenético D. piscinarius. Houve correlação positiva intermediária, avaliada pelo coeficiente de Pearson, entre a abundância parasitária de D. piscinarius e comprimento padrão (r=0,43) e peso (r=0,51) dos hospedeiros.Palavras-chave: Peixe de água doce, corvina, ecologia parasitária, Austrodiplostomum compactum, Diplectanum piscinarius.

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“…In Brazil, there are many records of helminth parasites in this host, including anisakid species (MARTINS et al, 2000;TAVARES et al, 2007;EIRAS et al, 2010;DIAS et al, 2011;MELO et al, 2014;RODRIGUES et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Anisakid larva parasitizing Plagioscion squamosissimus in Marajó Bay and Tapajós River, state of Pará, Brazil

Fontenelle

Knoff

Felizardo

et al. 2016

Rev. Bras. Parasitol. Vet.

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In November 2014 and May 2015, a total of 44 specimens of the South American silver croaker Plagioscion squamosissimus were collected: 30 in Marajó Bay and 14 in the Tapajós River, state of Pará, Brazil. The aim was to investigate the presence of anisakid nematodes and determine their parasitism indices and sites of infection, because of their importance regarding health inspection. Sixty-nine Anisakis sp. larvae were found; among them, 16 larvae in seven fish collected in Marajó Bay and 53 larvae in four fish in the Tapajós River. The parasitism indices of the nematodes collected from the fish in Marajó Bay comprised prevalence (P) of 23%, mean infection intensity (MI) of 2.28, mean abundance (MA) of 0.53, range of infection (RI) of 1-4, and infection site (IS) in the mesentery. The fish from the Tapajós River showed P = 28%, MI = 13.2, MA = 3.8, RI = 1-22, and IS = mesentery and intestine. To assist in taxonomic identification, images of the specimens obtained through optical microscopy with Nomarski's interferential contrast system and scanning electron microscopy were used. This is the first record of Anisakis sp. parasitizing P. squamosissimus.Keywords: Sciaenidae, freshwater fish, Anisakidae, Northern Brazil. ResumoEm novembro de 2014 e maio de 2015, foi coletado um total de 44 espécimes de pescada branca, Plagioscion squamosissimus, sendo 30 na Baía de Marajó e 14 no Rio Tapajós, Estado do Pará, Brasil, com o objetivo de pesquisar a presença de nematoides anisaquideos e fornecer os índices parasitários, assim como os sítios de infecção, devido a sua importância na inspeção sanitária. Encontrou-se um total de 69 larvas de Anisakis sp., sendo 16 em 7 peixes coletados da Baía de Marajó e 53 larvas em 4 peixes do Rio Tapajós. Os índices parasitários desses nematoides coletados dos peixes da Baía de Marajó apresentaram prevalência (P) de 23%, intensidade média (IM) de 2,28, abundância média (AM) de 0,53, amplitude de variação da intensidade de infecção (AI) de 1-4 e sítio de infecção (SI) o mesentério; e os do Rio Tapajós P = 28%, IM = 13,2, AM = 3,8, AI = 1-22 e SI = mesentério e intestino. Para auxiliar na identificação taxonômica foram utilizadas imagens obtidas dos espécimes por microscopia ótica com sistema de contraste interferencial de Nomarski e microscopia eletrônica de varredura. Esse é o primeiro registro de Anisakis sp. parasitando P. squamosissimus.Palavras-chave: Sciaenidae, peixe de água doce, Anisakidae, Norte do Brasil.

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Description and prevalence of Thynnascaris sp. larvae Dollfus, 1933 (Nematoda: Anisakidae) in Plagioscion squamosissimus Heckel, 1840 from Volta Grande Reservoir, State of Minas Gerais, Brazil

Cited by 8 publications

References 13 publications

Characterization of the acute inflammatory response in the hybrid tambacu (Piaractus mesopotamicus male × Colossoma macropomum female) (Osteichthyes)

Characterization of the acute inflammatory response in the hybrid tambacu (Piaractus mesopotamicus male × Colossoma macropomum female) (Osteichthyes)

Metazoan parasites of Plagioscion squamosissimus, an invasive species in the Tietê River, São Paulo, Brazil

Anisakid larva parasitizing Plagioscion squamosissimus in Marajó Bay and Tapajós River, state of Pará, Brazil

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