Description of 14 new capsular types of Streptococcus suis

Gottschalk, Marcelo; Higgins, Robert; Jacques, Mario; Mittal, K. R.; Henrichsen, Jørgen

doi:10.1128/jcm.27.12.2633-2636.1989

Cited by 187 publications

(94 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Strep. suis is a very heterogenic species in which 34 serotypes have been described (Gottschalk et al 1989;Gottschalk et al 1991a;Gottschalk et al 1991b), but only few of these are commonly present in lesions. Serotype 2, the most important clinical serotype, was not found in the present investigation.…”

Section: Discussionmentioning

confidence: 99%

The Gram-positive tonsillar and nasal flora of piglets before and after weaning

et al. 2001

View full text Add to dashboard Cite

Aims: To investigate Gram‐positive nasal and tonsillar microbial flora of piglets before and after weaning. Methods and Results: The nasal and tonsillar Gram‐positive bacterial flora of 20 non‐weaned piglets (2 weeks of age) and 20 weaned piglets (6 weeks of age), obtained from four different piggeries, was quantified by culture and identified by tDNA‐PCR. The most widely occurring species from nasal conchae before as well as after weaning in the different piglets investigated were Streptococcus suis and Rothia nasimurium. After weaning a wide variety of Lactobacillus species appeared but in low numbers. In the tonsils, Strep. suis, Strep. dysgalactiae, S. hyicus, S. aureus, Arcanobacterium pyogenes and Actinomyces hyovaginalis were the species isolated from the largest number of pigs before and after weaning. S. aureus and most lactobacilli became more prevalent after weaning. Bacteria not known to be associated with pigs found in the present study included R. nasimurium, Strep. gallolyticus, Pediococcus pentosaceus and some Lactobacillus species. Conclusions: Over 30 different Gram‐positive bacterial species may occur in nasal conchae and tonsils of unweaned piglets at 2 weeks of age and of 6‐week‐old weaned piglets. Significance and Impact of the Study: This study demonstrated that weaning is associated with changes in prevalence of only a small minority of the highly diversified bacterial flora of the nares and tonsils of pigs.

show abstract

Section: Discussionmentioning

confidence: 99%

The Gram-positive tonsillar and nasal flora of piglets before and after weaning

et al. 2001

View full text Add to dashboard Cite

show abstract

“…After being introduced into E. coli strain MC1061 (Casadaban & Cohen, 1980), the direction and sequences of the cloned genes were verified by PCR and sequencing. The resultant expression vectors were then introduced into unencapsulated S. suis isolates, and the restoration of capsular expression was examined by the coagglutination test and transmission electron microscopy (TEM) as described previously (Gottschalk et al, 1989;Jacques et al, 1990;Han et al, 2001;Lakkitjaroen et al, 2011).…”

Section: Construction Of Cps Gene Expression Vectors and Complementatmentioning

confidence: 99%

Capsule loss or death: The position of mutations among capsule genes sways the destiny ofStreptococcus suis

Lakkitjaroen

Takamatsu

Okura

et al. 2014

FEMS Microbiol Lett

View full text Add to dashboard Cite

Streptococcus suis, an emerging zoonotic pathogen, is responsible for various diseases in swine and humans. Most S. suis strains from clinical cases possess a group of capsular polysaccharide synthesis (cps) genes and phenotypically express capsular polysaccharides (CPs). Although CPs are considered to be an important virulence factor, our previous study showed that many S. suis isolates from porcine endocarditis lost their CPs, and some of these unencapsulated isolates had large insertions or deletions in the cps gene clusters. We further investigated 25 endocarditis isolates with no obvious genetic alterations to elucidate the unencapsulation mechanisms and found that a single-nucleotide substitution and frameshift mutation in two glycosyltransferase genes (cps2E and cps2F) were the main causes of the capsule loss. Moreover, mutations in the genes involved in side-chain formation (cps2J and cps2N), polymerase (cps2I), and flippase (cps2O) appeared to be lethal; however, these lethal effects were relieved by mutations in the cps2EF region. As unencapsulation and even the death of individual cells have recently been suggested to be beneficial to the pathogenesis of infections, the results of the present study provide a further insight into understanding the biological significance of cps mutations during the course of S. suis infections.

show abstract

“…In humans, S. suis type 2 can cause endocarditis, septicemia, meningitis and permanent hearing loss [1,19]. To date 35 serotypes of S. suis have been described (types 1/2, and 1 through 34) [3,4,5,12,19]. Type 2 is the most often isolated and the most frequently associated with dis-ease.…”

Section: Introductionmentioning

confidence: 99%

Hybridization analysis of the gene encoding a hemolysin (suilysin) of Streptococcus suis type 2: evidence for the absence of the gene in some isolates

Okwumabua

1999

FEMS Microbiology Letters

View full text Add to dashboard Cite

A hemolysin gene was cloned from a virulent strain of Streptococcus suis type 2 strain 1933. Analysis of the gene and its product revealed that it is identical to a previously reported hemolysin (suilysin) of S. suis type 2. Southern hybridization analysis of the digested total genomic DNA from S. suis with the cloned hemolysin DNA sequences as probe indicated that the hemolysin gene is present as a single copy on the genome. Genomic DNA of 63 isolates of S. suis encompassing all known serotypes were examined by DNA hybridization and polymerase chain reaction (PCR) studies for the presence of the hemolysin gene homolog. The results of both techniques were identical and demonstrated the absence of the hemolysin gene in some isolates. In DNA hybridization studies, three DNA probes derived from the hemolysin encoding gene were used. Results showed that sequences encoding the C-terminal 257 amino acid residues (Probe 1) were the most conserved and hybridized to a 1.2 kb fragment in 32 (51%) strains and a 4.0 kb fragment in 23 (36%) strains respectively. Thus, Probe 2 hybridized to the DNA of 55 (87%) of the isolates tested. The first probe (Probe 1) comprising almost the entire hemolysin gene and the third probe (Probe 3) which consisted of the N-terminal sequences hybridized only to a 4.0 kb fragment in 23 (36%) of the strains tested. Eight (13%) of the strains tested were hybridization and PCR negative. The hybridization of the C-terminal end sequences (Probe 2) to the 1.2 kb fragment in 32 (51%) of the strains and the lack of hybridization of the probes to eight (13%) strains may suggest the presence of different types of hemolysin molecule in S. suis strains. ß

show abstract

Description of 14 new capsular types of Streptococcus suis

Cited by 187 publications

References 22 publications

The Gram-positive tonsillar and nasal flora of piglets before and after weaning

The Gram-positive tonsillar and nasal flora of piglets before and after weaning

Capsule loss or death: The position of mutations among capsule genes sways the destiny ofStreptococcus suis

Hybridization analysis of the gene encoding a hemolysin (suilysin) of Streptococcus suis type 2: evidence for the absence of the gene in some isolates

Contact Info

Product

Resources

About