Description of a new species of soil algae, Parietochloris grandis sp. nov., and study of its fatty acid profiles under different culturing conditions

et al. 2019

Journal of Phycology

Self Cite

A novel freshwater strain of Coccomyxa elongata (MZ–Ch64) was isolated from the Zaporizhia region, Ukraine. The identification was based on the phylogenetic analysis of SSU rDNA gene and ITS1–5.8S rDNA–ITS2 region and predicted secondary structure of the ITS2. Phylogenetic analysis placed this strain in the Coccomyxa group, within the class Trebouxiophyceae. The novel strain MZ–Ch64 formed a strongly supported lineage closest with C. elongata. The MZ–Ch64 strain differed from the morphological description of the species by the size of vegetative cells and absence of small mucilaginous caps at one end of the cell. A number of experiments with different concentrations of phosphate and nitrate were conducted to evaluate changes in the resulting fatty acid profiles and biomass productivity. The fatty acid profile and total fatty acids varied significantly under different nutrient deficiencies. The dominant fatty acid during cultivation on standard BBM medium, as well as in phosphorus‐depleted conditions, was oleic acid (to 48.0%–54.6% of total fatty acids). Absence of nitrogen alone, and absence of both nitrogen and phosphorus, led to an increase of palmitic acid (to 24.7%–25.6%), cis‐7‐hexadecenoic acid (to 14.8%) and α‐linolenic acid (to 9.1%–10.1%) in comparison with the control sample. The greatest variation was found for oleic acid (31.9%–54.6%). Thus, this strain can be considered as a potential producer of oleic acid or cis‐7‐hexadecenoic and α‐linolenic acids for biotechnological applications.

“…Sequencing was performed using the Genetic Analyzer 3,500 sequencer (Applied Biosystems, USA). For sequencing we used internal primers provided by Maltsev et al (2018).…”

Section: Methodsmentioning

confidence: 99%

Fatty Acid Content and Profile of the Novel Strain of Coccomyxa elongata (Trebouxiophyceae, Chlorophyta) Cultivated at Reduced Nitrogen and Phosphorus Concentrations

et al. 2019

Journal of Phycology

Self Cite

“…[3][4][5][6]. Of significant interest to the food and feed industry is the ability of microalgae to produce carotenoids such as β-carotene, astaxanthin, lutein, as well as lipids containing a wide variety of fatty acids, including long-chain polyunsaturated fatty acids (PUFAs) from the omega-3 and omega-6 [7][8][9][10][11][12][13] group.…”

Section: Introductionmentioning

confidence: 99%

Influence of Light Conditions on Microalgae Growth and Content of Lipids, Carotenoids, and Fatty Acid Composition

Kulikovskiy

et al. 2021

Biology

Self Cite

214

Microalgae are a valuable natural resource for a variety of value-added products. The growth of microalgae is determined by the impact of many factors, but, from the point of view of the implementation of autotrophic growth, light is of primary importance. This work presents an overview of the influence of light conditions on the growth of microalgae, the content of lipids, carotenoids, and the composition of fatty acids in their biomass, taking into account parameters such as the intensity, duration of lighting, and use of rays of different spectral composition. The optimal light intensity for the growth of microalgae lies in the following range: 26−400 µmol photons m−2 s−1. An increase in light intensity leads to an activation of lipid synthesis. For maximum lipid productivity, various microalgae species and strains need lighting of different intensities: from 60 to 700 µmol photons m−2 s−1. Strong light preferentially increases the triacylglyceride content. The intensity of lighting has a regulating effect on the synthesis of fatty acids, carotenoids, including β-carotene, lutein and astaxanthin. In intense lighting conditions, saturated fatty acids usually accumulate, as well as monounsaturated ones, and the number of polyunsaturated fatty acids decreases. Red as well as blue LED lighting improves the biomass productivity of microalgae of various taxonomic groups. Changing the duration of the photoperiod, the use of pulsed light can stimulate microalgae growth, the production of lipids, and carotenoids. The simultaneous use of light and other stresses contributes to a stronger effect on the productivity of algae.

“…2018), Parietochloris grandis (Maltsev et al. 2018), and Coccomyxa elongata (Maltsev et al. 2019b) showed that nutrient concentration in the medium strongly influences biomass accumulation.…”

Section: Discussionmentioning

confidence: 99%

“…For sequencing 18S rDNA gene, internal primers 18S–MZ–F and 18S–MZ–R from Maltsev et al. (2018) were used.…”

Section: Methodsmentioning

confidence: 99%

A New Species of Freshwater Algae Nephrochlamys yushanlensis sp. nov. (Selenastraceae, Sphaeropleales) and Its Lipid Accumulation during Nitrogen and Phosphorus Starvation

et al. 2021

Journal of Phycology

Self Cite

The new species Nephrochlamys yushanlensis sp. nov. is described from a freshwater plankton sample. A comparison of morphology, 18S rDNA gene and ITS2 sequences, and fatty acid profiles showed that the novel strain represents a new lineage within the genus Nephrochlamys. For the first time with a member of the Selenastraceae, experiments with phosphate and nitrate deprivation were conducted to evaluate changes in biomass, lipid and triacylglycerol (TAGs) accumulation, and composition of fatty acids. Biomass dry weight under simultaneous nitrogen and phosphorus depletion was 1.73 g · L−1, which is significantly lower than the 2.41 g · L−1 observed in the control. All conditions of nutrient restriction significantly increased the lipid content in comparison with the control. The largest increase in the total lipid content, reaching 58.64% DW per cell at the end of cultivation, occurred with nitrogen deficiency. Significant increases in TAGs content (to 23.69% and 21.74%, respectively) occurred in phosphorus‐ and nitrogen‐depleted conditions in comparison to the control (16.90%). Oleic (49.8–64.1%), palmitic (21.1–22.7%), and linoleic (8.6–10.3%) acids were the dominant fatty acids when cultured on standard BBM medium, as well as with the shortage of nutrients. Phosphorus deprivation as well as absence of both nitrogen and phosphorus led to the appearance of FAMEs α‐linolenic (1.5–4.1%) and stearidonic (1.0–1.8%) acids. In general, FAME profiles revealed that the relative percentage of saturated and monounsaturated fatty acids increased (88.9% of total fatty acids) in nitrogen‐depletion conditions, suggesting this strain may be suitable for biodiesel production.