Description of a new species ofAuriculostoma(Digenea: Allocreadiidae) fromCharacidium heirmostigmata(Characiformes: Crenuchidae) from Argentina, using morphological and molecular data

Abstract

During a parasitological survey of fishes at Iguazu National Park, Argentina, specimens belonging to the allocreadiid genus Auriculostoma were collected from the intestine of Characidium heirmostigmata. The erection of the new species is based on a unique combination of morphological traits as well as on phylogenetic analysis. Auriculostoma guacurarii n. sp. resembles four congeneric species – Auriculostoma diagonale, Auriculostoma platense, Auriculostoma tica and Auriculostoma totonacapanensis – in having smooth and oblique testes, but can be distinguished by a combination of several morphological features, hosts association and geographic distribution. Morphologically, the new species can be distinguished from both A. diagonale and A. platense by the egg size (bigger in the first and smaller in the last); from A. tica by a shorter body length, the genital pore position and the extension of the caeca; and from A. totonacapanensis by the size of the oral and ventral sucker and the post-testicular space. Additionally, one specimen of Auriculostoma cf. stenopteri from the characid Charax stenopterus (Characiformes) from La Plata River, Argentina, was sampled and the partial 28S rRNA gene was sequenced. The phylogenetic analysis revealed that A. guacurarii n. sp. clustered with A. tica and these two as sister taxa to A. cf. stenopteri. The new species described herein is the tenth species in the genus and the first one parasitizing a member of the family Crenuchidae.

Discovery of a new genus and species of Allocreadiidae (Trematoda) in Mexico: Mesoamericatrema magnisacculus n. gen. n. sp

“…In particular, the number of oral papillae can be variable, however, the papillae that are constant in number are the internal papillae close to the mouth (4) and the posterior external papillae (5). This peculiarity of the number of internal and posterior oral papillae had already been observed in other genera such as Creptotrema (Razo-Mendivil et al, 2014; Hernández-Mena et al, 2016 ; 2019; Montes et al, 2021 ), Paracreptotrema ( Choudhury et al, 2006 ; Perez-Ponce de León et al, 2016), Paracreptotrematoides (Perez-Ponce de León et al, 2016), Pseudoparacreptotrema (Perez-Ponce de León et al, 2016; 2020) and Wallinia ( Hernández-Mena et al, 2019 ), which usually also have 4 internal papillae and 5 external posterior papillae, which suggests that so this character may be associated with this group of allocreadiids and may even be a synapomorphy for this large Neotropical clade. As a result, we suggest that it be included in the general diagnosis of these aforementioned genera.…”

Three new species of Creptotrema (Trematoda, Allocreadiidae) with an amended diagnosis of the genus and reassignment of Auriculostoma (Allocreadiidae), based on morphological and molecular evidence

“…n. Gymnotus maculosus Albert & Miller, 1995 Gymnotiformes, Gymnotidae Orosí River, Costa Rica [ 25 ] Creptotrema guacurarii comb. n. Characidium heirmostigmata da Graça & Pavanelli, 2008 Characiformes, Crenuchidae Arrechea Stream, Iguazu River above the waterfalls, Iguazu National Park, Misiones, Argentina [ 41 ] Creptotrema conconae n. sp. Imparfinis mirini Haseman, 1911 Siluriformes, Heptapteridae Upper Paraná River basin, São Paulo State, Brazil Present study Creptotrema schubarti n. sp.…”

“… Imparfinis mirini Siluriformes Upper Paraná River basin, Brazil OK044374 OK075288 Present study Creptotrema funduli ( species inquirenda ) Fundulus notatus Cyprinodontiformes Biloxi River, Mississippi, USA JQ425256 – [ 15 ] Creptotrema guacurarii comb. n. (= Auriculostoma guacurarii ) Characidium heirmostigmata Characiformes Iguazú National Park, Arrechea Stream, Argentina MN822004 – [ 41 ] Creptotrema lobata comb. n. (= Auriculostoma lobata ) Brycon guatemalensis Characiformes El Managal Lagoon, Tenosique, Mexico KX954170 – KX954173 – [ 24 ] Creptotrema megacetabularis n. sp.…”

“…n. (= Auriculostoma cf. stenopteri ) Charax stenopterus Characiformes La Plata River, Argentina MN822005 – [ 41 ] Creptotrema tica comb. n. (= Auriculostoma tica ) Gymnotus maculosus Gymnotiformes Orosí River, Costa Rica MH997001 – MH997002 – [ 25 ] Creptotrema totonacapanense comb.…”

An integrative taxonomy study reveals a rare new species of the genus Creptotrema (Trematoda: Allocreadiidae) in an endangered frog in South America

“…2021; Liquin et al . 2022), with 16 of those occurring in South America – that is, the type-species Creptotrema creptotrema Travassos, Artigas & Pereira, 1928, Creptotrema conconae Franceschini, Aguiar, Zago, Yamada, Ebert & Silva, 2021, Creptotrema diagonale (Curran, Tkach & Overstreet, 2011), Creptotrema foliaceum (Curran, Tkach & Overstreet, 2011), Creptotrema guacurarii Montes, Barneche, Croci, Balcazar, Almirón, Martorelli & Pérez-Ponce de León, 2021, Creptotrema lamothei Curran, 2008, Creptotrema lynchi Brooks, 1976, Creptotrema macrorchis (Szidat, 1954), Creptotrema megacetabulare Franceschini, Aguiar, Zago, Yamada, Ebert & Silva, 2021, Creptotrema ocloye (Liquin, Gilardoni, Cremonte, Saravia, Cristóbal & Davies, 2022) n. comb., Creptotrema pati Lunaschi, 1985, Creptotrema paraense Vicente, Santos & Souza, 1978, Creptotrema platense (Szidat, 1954), Creptotrema schubarti Franceschini, Aguiar, Zago, Yamada, Ebert & Silva, 2021, Creptotrema stenopteri (Mañé-Garzón & Gascón, 1973), and Creptotrema sucumbiosa Curran, 2008.…”