Description of a novel marine bacterium, Vibrio hyugaensis sp. nov., based on genomic and phenotypic characterization

Genomic evidence that Vibrio inhibens is a heterotypic synonym of Vibrio jasicida

“…Balcazar et al (2012) reported that V. inhibenscould be differentiated from related species on the basis of the lack of mannose assimilation and sucrose fermentation. Phenotypic characterization carried out using matching experimental methods found two V. jasicidastrains to be negative or weakly positive for both tests (Table S1; Urbanczyk et al, 2015). Overall phenotypic character was found to be very similar between CECT 7692 T and two representative V. jasicida strains.…”

“…CECT 7692 T showed high ANIb values to three Vibrio hyugaensis strains included in the analysis, between 93.4 % and 93.6 %, however these ANIb values are lower than the 95 % recommended for classifying strains as representing members of the same bacterial species. V. hyugaensis is a recently described species in the Harveyi clade (Urbanczyk et al, 2015), which was predicted to be recently separated from V. jasicida (Urbanczyk et al, 2014). ANIb values between CECT 7692 T and other analyzed strains of the Harveyi clade were 89.4 % or lower.…”

“…Results of ANIm, orthoANI and GGDC calculations (Tables S3-S5) support classification of CECT 7692 T and the five analyzed V. jasicida strains as members of the same species. The results of Tetra calculations (Table S6) show a high coefficient (>0.99) between CECT 7692 T and strains classified as members of V. jasicida, V. hyugaensis, Vibrio campbellii, Vibrio owensii and Vibrio rotiferianus; however the Tetra coefficient has been previously shown to be of limited use for taxonomic classification of bacteria of the Harveyi clade (Urbanczyk et al, 2015). Based on the results of ANIb, ANIm, orthoANI and GGDC calculations it can be concluded that the strain CECT 7692 T and the five analyzed strains of V. jasicida, including V. jasicida LMG 25398 T , should be classified as members of the same species.…”

“…The CECT 7692 T 16S rRNA gene sequence was amplified as described previously (Urbanczyk et al, 2015); the sequence was deposited in GenBank under the accession number KU360274. The CECT 7692 T genome sequence had 13 fragments matching the PCR amplified 16S sequence, which can be explained by the presence of multiple rRNA operons in the V. inhibens CECT 7692 T genome (data not shown), similar to the situation in other members of the family Vibrionaceae (Gonzalez- Escalona et al, 2005;Vezzi et al, 2005).…”

“…Further comparison of the strain reported by Balcazar et al (2012) and V. inhibens CECT 7692 T involved phenotypic analysis. Phenotypic tests were performed using API 20NE test (bioMerieux) in triplicates, according to the manufacturer's instructions, at 26 C for 24 h and 48 h. Salt and temperature tolerance tests were performed as described previously (Urbanczyk et al, 2015). Results of the tests show that CECT 7692 T has phenotypic characters corresponding to the type strain of V. inhibens described by Balcazar et al (2012) (Table S1, available in the online Supplementary Material).…”