Description of Aphelenchoides stellatus n. sp. (Nematoda: Aphelenchoididae) found in packaging wood from Japan

Abstract

Aphelenchoides stellatus n. sp. is described and figured. The new species was isolated from packaging wood from Japan imported to Ningbo harbour, China. The new species has a body length of 485-533 g m (males) and 547-699 p m (females). The cuticle is weakly annulated and there are four lines in the lateral field. The stylet is 9-11 p m long and has small basal swellings. The excretory pore is located posterior to the nerve ring. Spicules smoothly curved, rose-thorn shaped. Apex and rostrum round, only slightly offset, dorsal limb 19-21 p m long. Male tail bearing six (2 + 2 + 2) caudal papillae. The female spermatheca is axial and oblong with round sperm present in multiple rows. Both male and female tail pegs have 3-4 processes, appearing star-like under SEM. The new species belongs to the Group 3 category of Aphelenchoides species. Phylogenetic analyses based on full length ITS and 28S D2/D3 region of rDNA confirmed its morphological status as a new species.

Molecular and Morphological Characterization of Aphelenchoides fuchsi sp. n. (Nematoda: Aphelenchoididae) Isolated from Pinus eldarica in Western Iran

“…In the phylograms, Aphelenchoides appears to be polyphyletic, a finding that agrees with previous studies (Zhao et al, 2008;Rybarczyk-Myd1owska et al, 2012;Wang et al, 2013;Fang et al, 2014aFang et al, , 2014bEsmaeili et al, 2016). Aphelenchoides, Laimaphelenchus, and Schistonchus s.l.…”

“…n. were compared with those of other aphelenchids species available in GenBank (see Table 1 for selected sequences of SSU and Table 2 for LSU D2-D3) using BLAST homology search program. Outgroup was chosen according to previous published data (Fang et al, 2014a(Fang et al, , 2014bEsmaeili et al, 2016). The newly obtained and published sequences were aligned using MAFFT ver.…”

“…Currently, species discrimination in Aphelenchoides is mainly based on morphology and morphometrics. In recent years, several species of the genus were described related to the pine trees in different countries, e.g., China (Cheng et al, 2009;Zhuo et al, 2010) and the United States (Kaisa, 2000), and some species isolated form packaging wood in South Korea (Cui et al, 2011;Fang et al, 2014b), India (Bina Chanu et al, 2013), South Africa (Wang et al, 2013), and Japan (Fang et al, 2014a). Recently a new species of Aphelenchoides, Aphelenchoides huntensis Esmaeili, Fang, Li, and Heydari, 2016 is described in association with pine trees in Iran.…”

Description of Aphelenchoides macrospica n. sp. (Nematoda: Aphelenchoididae) from Northwestern Iran

“…is grouped with other genera of Aphelenchoidinae, i.e., Ficophagus Davies, Ye, Kanzaki, Bartholomaeus, Zeng, and Giblin-Davis, 2015, Martininema Davies, Ye, Kanzaki, Bartholomaeus, Zeng, and Giblin-Davis, 2015, Laimaphelenchus Fuchs, 1937, and Schistonchus s.s. Davies, Ye, Kanzaki, Bartholomaeus, Zeng, and Giblin-Davis, 2015, in clade 2. According to our molecular analysis, the members of the genus Aphelenchoides appears to be paraphyletic, a finding that agrees with previous studies (Zhao et al, 2008;Rybarczyk-Mydl À owska et al, 2012;Wang et al, 2013;Fang et al, 2014aFang et al, , 2014bMiraeiz et al, 2015;Esmaeili et al, 2016aEsmaeili et al, , 2016bGolhasan et al, 2016). Furthermore Aphelenchoides, Laimaphelenchus, and Schistonchus s. l cannot be clearly separated, possibly implying that all the genera share a recent common ancestor (Zeng et al, 2007).…”

Description of Sheraphelenchus parabrevigulonis n. sp. (Nematoda: Aphelenchoididae) in pine wood packaging from Italy and redescription of S. sucus in onion bulbs from South Korea, isolated at Ningbo, China

“…The tree topology for each gene was determined separately using MrBayes 3.1.2 (Ronquist & Huelsenbeck, 2003). The running programs of the trees were as described by Fang et al (2014). The consensus trees were selected to represent the phylogenetic relationships with branch length and support level, and visualised using TreeGraph2 (Stöver & Müller, 2010).…”