Description of Babesia duncani n.sp. (Apicomplexa: Babesiidae) from humans and its differentiation from other piroplasms

Conrad, Patricia A.; Kjemtrup, Anne; Carreno, Ramon A.; Thomford, John W.; Wainwright, Katlyn; Eberhard, Mark L.; Quick, Rob; Telford, Sam R.; Herwaldt, Barbara L.

doi:10.1016/j.ijpara.2006.03.008

Cited by 181 publications

(139 citation statements)

References 37 publications

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“…It has also been shown that B. conradae is closely related to Babesia spp. in humans in the United States (8,20,21). The piroplasm that is most closely related to B. conradae is B. duncani, isolated from a human in the western United States (8,40).…”

Section: Discussionmentioning

confidence: 99%

Babesia lengau sp. nov., a Novel Babesia Species in Cheetah ( Acinonyx jubatus , Schreber, 1775) Populations in South Africa

et al. 2010

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In a previous paper, we reported on a large number of cheetah blood specimens that gave positive signals only for Babesia and/or Theileria genus-specific probes on the reverse line blot (RLB) assay, indicating the presence of a novel species or variant of an existing species. Some of these specimens were investigated further by microscopic, serological, sequencing, and phylogenetic analyses. The near-full-length 18S rRNA genes of 13 samples, as well as the second internal transcribed spacer (ITS2) region, were amplified, cloned, and sequenced. A species-specific RLB probe, designed to target the hypervariable V4 region of the 18S rRNA gene for detection of the novel Babesia sp., was used to screen an additional 137 cheetah blood specimens for the presence of the species. The prevalence of infection was 28.5%. Here we describe the morphology and phylogenetic relationships of the novel species, which we have named Babesia lengau sp. nov.

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Section: Discussionmentioning

confidence: 99%

Babesia lengau sp. nov., a Novel Babesia Species in Cheetah ( Acinonyx jubatus , Schreber, 1775) Populations in South Africa

et al. 2010

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“…However, the nomenclature of human-infecting Babesia parasites has recently become more complex due to the emergence of new isolates in Europe, the United States, Africa, South America, and Asia. The classification of humaninfecting Babesia species was initially dependent on morphology and host specificity, although later studies based on molecular techniques indicated that these criteria are inadequate (114,459).…”

Section: Babesia Sppmentioning

confidence: 99%

Importance of Nonenteric Protozoan Infections in Immunocompromised People

et al. 2010

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SUMMARY There are many neglected nonenteric protozoa able to cause serious morbidity and mortality in humans, particularly in the developing world. Diseases caused by certain protozoa are often more severe in the presence of HIV. While information regarding neglected tropical diseases caused by trypanosomatids and Plasmodium is abundant, these protozoa are often not a first consideration in Western countries where they are not endemic. As such, diagnostics may not be available in these regions. Due to global travel and immigration, this has become an increasing problem. Inversely, in certain parts of the world (particularly sub-Saharan Africa), the HIV problem is so severe that diseases like microsporidiosis and toxoplasmosis are common. In Western countries, due to the availability of highly active antiretroviral therapy (HAART), these diseases are infrequently encountered. While free-living amoebae are rarely encountered in a clinical setting, when infections do occur, they are often fatal. Rapid diagnosis and treatment are essential to the survival of patients infected with these organisms. This paper reviews information on the diagnosis and treatment of nonenteric protozoal diseases in immunocompromised people, with a focus on patients infected with HIV. The nonenteric microsporidia, some trypanosomatids, Toxoplasma spp., Neospora spp., some free-living amoebae, Plasmodium spp., and Babesia spp. are discussed.

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“…Because internal transcribed spacers (ITSs) have great variability in both nucleotide and length, ITS sequences were used for discriminating different geographic isolates of piroplasmids, identifying new species, and differentiating between piroplasm species and subspecies (Holman et al 2003, Baneth et al 2004, Conrad et al 2006, Aktas et al 2007, Liu et al 2008.…”

Section: Introductionmentioning

confidence: 99%

A PCR method targeting internal transcribed spacers: the simultaneous detection of Babesia bigemina and Babesia bovis in cattle

Liu

Guan

Liu

et al. 2014

Acta Parasitologica

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In this study, two pairs of oligonucleotide primers were designed according to the nucleotide sequence of the internal transcribed spacers (ITSs) of Babesia bigemina and B. bovis isolates from China. The primers were used in a multiplex PCR to detect parasite DNA in blood samples from cattle. There was no cross reactions with B. ovata, B. major, B. sp. Kashi, Theileria annulata, T. sergenti, T. sinensis or normal bovine DNA. The sensitivity of multiplex PCR assay was 1 pg and 10 pg DNA for B. bigemina and B. bovis, respectively. A total of 260 field blood samples collected from cattle in five provinces of China were analyzed by multiplex PCR and light microscopy. PCR testing revealed that 7.3% (19/260) and 5.8% (15/260) of cattle were positive for B. bigemina and B. bovis and 1.2% (3/260) of cattle were co-infected with B. bigemina and B. bovis. Using light microscopy, 2.3% (6/260) and 1.5% (4/260) of cattle were infected by B. bigemina and B. bovis, respectively, and no co-infection was found. The results showed that the multiplex PCR developed in the present study could be an alternative diagnostic tool for the detection of B. bovis and B. bigemina infection in cattle.

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Description of Babesia duncani n.sp. (Apicomplexa: Babesiidae) from humans and its differentiation from other piroplasms

Cited by 181 publications

References 37 publications

Babesia lengau sp. nov., a Novel Babesia Species in Cheetah ( Acinonyx jubatus , Schreber, 1775) Populations in South Africa

Babesia lengau sp. nov., a Novel Babesia Species in Cheetah ( Acinonyx jubatus , Schreber, 1775) Populations in South Africa

Importance of Nonenteric Protozoan Infections in Immunocompromised People

A PCR method targeting internal transcribed spacers: the simultaneous detection of Babesia bigemina and Babesia bovis in cattle

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