Description of Basiria khouzestanensis n. sp. (Nematoda: Tylenchidae) from Iran and its phylogenetic relationships with other species in the family

Abstract

Basiria khouzestanensis n. sp., recovered from the rhizosphere of orange in Khouzestan Province, south-western Iran, is described and illustrated based on morphological, morphometric and molecular data. The new species is characterized by having short body length (430–635 µm), lateral fields composed of four incisures, with non areolated bands, cephalic region smooth and continuous with body contour, stylet 9–12.5 µm long, basal bulb short and pyriform to slightly cylindrical, V = 63.3 (58–65.5), spermatheca non-offset, tail elongate-conoid, tail tip with various shapes and males with 16.5 (15–18) μm long spicules. Morphologically, the new species is similar to five known species of the genus: B. babhi, B. brevia, B. graminophila, B. lauta and B. shahidi. Morphological comparisons with these species are discussed. The sequence of the D2–D3 expansion segments of 28S rDNA gene for the new species was also used for molecular phylogenetic analysis. The phylogenetic relationships of the new species in relation to representatives of the family Tylenchidae, obtained from Bayesian inference (BI) analysis of the D2–D3 sequences, are presented and discussed. The new species clusters in a subclade containing three unidentified species of the genus Basiria from Iran with high support.

First Report of Three Tylenchidae Taxa from Southern Alberta, Canada

“…This clade further grouped with other species of Basiria (such as B. aberrans (Thorne) Siddiqi [58,59], B. gracilis (Thorne) Siddiqi [58,59], B. graminophila Siddiqi [14], B. khouzestanensis Eisvand, Farrokhi-Nejad, and Azimi [60]), Neothada Khan [61], and Neopsilenchus Thorne and Malek [62]. Our results support previous findings of Yaghoubi et al [55] that monodelphic genera of Boleodorinae are monophyletic.…”

Discopersicus hexagrammatus n. sp. (Rhabditida: Tylenchidae), the second species of the genus

“…Our 28S rDNA tree supported the position of Discopersicus in the subfamily Boleodorinae ( Khan, 1964 ) in close affinity with the genera Basiria and Boleodorus . The monophyly of the subfamily Boleodorinae was confirmed as concluded in former researches ( Eisvand et al, 2019 ; Hosseinvand et al, 2020a, b, c ; Panahandeh et al, 2019 ; Qing and Bert, 2018 ; Yaghoubi et al, 2016 ). The new species formed a strongly supported clade with Boleodorus spp., while, in Yaghoubi et al (2016) study, D. iranicus was placed inside Neopsilenchus spp.…”

“…Khuzestan province is located in southwest of Iran, and with arid and warm climate, has an important role for production of agricultural products in Iran. A large number of nematodes have been described from this province ( Azimi and Pedram, 2020 ; Eisvand et al, 2019 ; Ghaderi and Karegar, 2016 ; Hosseinvand et al, 2019, 2020a ; Panahandeh et al, 2019 ). Family Tylenchidae Örley, 1880 is one of the most plentiful and diverse nematode groups recovered in soil habitats, where they may represent up to 30% of the nematode abundance in any given soil sample ( Qing et al, 2018 ).…”

Morphological and molecular data of two species of the rare genera Thada Thorne, 1941 and Tenunemellus Siddiqi, 1986 (Nematoda: Tylenchidae) from Iran

“…The taxonomic studies on the members of the family Tylenchidae reported from Iran are well documented and discussed in Karegar (2018). Some other species of the genera Miculenchus Andrássy, 1959 (Panahandeh et al ., 2019a), Coslenchus Siddiqi, 1978 (Hosseinvand et al, 2019), Labrys Qing & Bert, 2018 (Panahandeh et al ., 2018, 2019b), Sakia Khan, 1964b (Panahandeh et al ., 2019c), Basiria Siddiqi, 1959 (Eisvand et al ., 2019) and Malenchus Andrássy, 1959 (Jalalinasab et al ., 2019) have since been reported or described from Iran through morphological and molecular studies. The present study aims to describe the newly recovered species, named Thada populus n.…”