Description of Desulfotomaculum sp. Groll as Desulfotomaculum gibsoniae sp. nov.

Abstract

Strain GrollT, isolated from fresh water, is a mesophilic, spore-forming, sulfatereducing bacterium that uses a large variety of substrates as electron donors ranging from simple organic compounds to long-chain fatty acids and several aromatic compounds. Sulfate, thiosulfate and sulf ite are used as electron acceptors. Homoacetogenic growth occurs under sulfate-free conditions. Substrate oxidation is usually complete, leading to CO,, but acetate or other fatty acids can accumulate at high substrate concentrations. The G+C content of the DNA is 5 4 8 mol%. Strain GrollT was found to be phenotypically and phylogenetically different from known members of the genus Desulfotomaculum. 165 rRNA gene sequence analyses show that this organism falls within the radiation of the genus Desulfotomaculum cluster and has < 96 YO sequence similarity to previously described species. The name Desulfotomaculum gibsoniae sp. nov. is proposed for this strain; the type strain is GrolIT (= DSM 72133.

Phylogeny of the alpha and beta subunits of the dissimilatory adenosine-5′-phosphosulfate (APS) reductase from sulfate-reducing prokaryotes – origin and evolution of the dissimilatory sulfate-reduction pathway

“…forming a basal-positioned group. The proposed 16S rRNA gene-based classification of the Desulfotomaculum and Desulfosporosinus members into the subclusters Ia-If and II (Kuever et al, 1999) (Fig. 1) was supported by the Apr trees (Figs 2 and 3, Supplementary Fig.…”

“…*Taxonomic classification of investigated SRP species is according to the Taxonomic outline of the prokaryotes, Bergey's Manual of Systematic Bacteriology, 2nd edition, release 5.0, May 2004 (http://dx.doi.org/10.1007/bergeysoutline) and Kuever et al (1999). DDSMZ strain numbers; 2, not deposited in a culture collection; T, type strain.…”

Desulfovibrio butyratiphilus sp. nov., a Gram-negative, butyrate-oxidizing, sulfate-reducing bacterium isolated from an anaerobic municipal sewage sludge digester

“…However, in the presence of sulfate as an electron acceptor, some sulfate-reducing bacterial species oxidize butyrate either completely to CO 2 or incompletely to acetate (Rabus et al, 2000). These sulfate-reducing bacterial species belong to the families Desulfobacteraceae (Cravo-Laureau et al, 2004;Kuever et al, 2005;Balk et al, 2008;Suzuki et al, 2008), Desulfohalobiaceae (Belyakova et al, 2006) and Syntrophobacteraceae (Beeder et al, 1995;Sievert & Kuever, 2000;Tanaka et al, 2000) in the class Deltaproteobacteria or to the genus Desulfotomaculum in the phylum Firmicutes (Daumas et al, 1988;Tasaki et al, 1991;Fardeau et al, 1995;Kuever et al, 1999;Vandieken et al, 2006).…”

Desulfotomaculum alcoholivorax sp. nov., a moderately thermophilic, spore-forming, sulfate-reducer isolated from a fluidized-bed reactor treating acidic metal- and sulfate-containing wastewater

“…The spores were located centrally. The cells (Fardeau et al, 1995); 5, D. gibsoniae DSM 7213 T (Kuever et al, 1999); 6, D. geothermicum DSM 3669 T (Daumas et al, 1988). All of the organisms shared the following characteristics: spore formation, spherical central or subterminal spores, sulfate (10 mM) reduction and oxidation of formate (10 mM), butyrate (10 mM), hexanoate (2 mM) and ethanol (10 mM) as electron donors.…”

“…The genus currently includes mesophilic, moderately thermophilic and thermophilic species (Werkman & Weaver, 1927;Campbell & Postgate, 1965;Vandieken et al, 2006). The previously described mesophilic (optimal temperature 25-40 u C) Desulfotomaculum species are D. ruminis (Campbell & Postgate, 1965), D. acetoxidans (Widdel & Pfennig, 1977), D. sapomandens (Cord-Ruwisch & Garcia, 1985), D. aeronauticum (Hagenauer et al, 1997), D. halophilum (Tardy-Jacquenod et al, 1998) and D. gibsoniae (Kuever et al, 1999). The thermophilic (optimal temperature 50-70 u C) species are D. alkaliphilum (Pikuta et al, 2000), D. australicum (Love et al, 1993), D. carboxydivorans (Parshina et al, 2005), D. geothermicum (Daumas et al, 1988), D. kuznetsovii (Nazina et al, 1989), D. luciae (Liu et al, 1997), D. nigrificans (Werkman & Weaver, 1927;Campbell & Postgate, 1965), D. putei (Liu et al, 1997), D. solfataricum (Goorissen et al, 2003), D. thermoacetoxidans (Min & Zinder, 1990), D. thermobenzoicum with two subspecies, namely subsp.…”