Description of four Apiotrichum and two Cutaneotrichosporon species isolated from guano samples from bat-inhabited caves in Japan

Abstract

Four new yeast species belonging to the genus Apiotrichum and two new yeast species belonging to Cutaneotrichosporon are described for strains isolated from guano samples from bat-inhabited caves in Japan. In 2005, we reported these isolates as Trichosporon species based on sequence analyses of the D1/D2 domain of large subunit (LSU) rRNA genes according to available basidiomycetous yeast classification criteria; however, to date, they have not been officially published as new species with descriptions. Their phylogenetic positions have been reanalysed based on comparison of internal transcribed spacer (ITS) region sequences (including the 5.8S rRNA gene) and the D1/D2 domain of the LSU rRNA gene with those of known species; we confirmed clear separation from previously described species. Physiological and biochemical properties of the isolates also suggest their distinctiveness. Therefore, we describe Apiotrichum akiyoshidainum (holotype JCM 12595T), Apiotrichum chiropterorum (JCM 12594T), Apiotrichum coprophilum (JCM 12596T), Apiotrichum otae (JCM 12593T), Cutaneotrichosporon cavernicola (JCM 12590T) and Cutaneotrichosporon middelhovenii (JCM 12592T) as new species. C. cavernicola showed particularly distinctive morphology including large inflated anomalous cells on the hyphae and germination from the cells, although clear clamp connections on the hyphae were not confirmed. Further study is needed to elucidate the morph of this species.

Airborne Fungi in Show Caves from Southern Spain

“…The presence of yeasts in the caves was related with the frequentation of people and animals [35]. Cutaneotrichosporon, Trichosporon and Cryptococcus species have been isolated from bat guano in caves in Japan [36,37]. Cutaneotrichosporon moniliiforme was the most abundant species in Gruta de las Maravillas (Sala de las Conchas) in summer, which was also identified in the air of Castañar de Ibor Cave [38].…”

Chromosome-level genome assemblies of Cutaneotrichosporon spp. (Trichosporonales, Basidiomycota) reveal imbalanced evolution between nucleotide sequences and chromosome synteny

“…Both yeast and hyphal cells were observed during vegetative growth of strain HIS471(Fig. S1 A, B), as was the case for the C. cavernicola standard strains [ 15 ]. The identical ITS sequence also supported a close relationship with the C. cavernicola standard strains (Fig.…”

“…Gene model annotation based on both de novo prediction and RNA-seq hints predicted approximately 7,300 to 7,800 genes (Table 2 ), a relatively smaller number than in C. oleaginosus [ 20 ] and Trichosporon asahii [ 21 ], but in the range for Trichosporonales spp. [ 15 ]. The number of genes in C. spelunceum HIS016 was smaller than in other strains, as was the genome size.…”

“…We previously reported the identity of several strains of Tremellomycetes yeasts found in bat-inhabited caves in Japan as Trichosporon [ 14 ]. Several strains were later described as Cutaneotrichosporon cavernicola , with strain HIS019 designated as the type strain [ 15 ]. Strains HIS002, HIS631, and HIS641 were also described as C. cavernicola with HIS019 (hereafter identified as “ C. cavernicola standard strains”).…”

Unveiling Trichosporon austroamericanum sp. nov.: A Novel Emerging Opportunistic Basidiomycetous Yeast Species

“…Currently, the Trichosporon genus comprises twelve species, with six of them capable of causing a diverse range of manifestations in human hosts, including superficial mycoses (e.g., white piedra, onychomycosis), summer-type hypersensitivity pneumonitis, esophageal lesions, fungemia, endocarditis, and other deep-seated infections [ 3 – 5 , 7 , 14 , 16 – 18 ]. Trichosporon asahii is the predominant species causing human invasive trichosporonosis, followed by Trichosporon inkin and Trichosporon asteroides [ 2 , 3 , 6 , 8 , 19 ].…”