Description of four novel species of Xenorhabdus, family Enterobacteriaceae: Xenorhabdus budapestensis sp. nov., Xenorhabdus ehlersii sp. nov., Xenorhabdus innexi sp. nov., and Xenorhabdus szentirmaii sp. nov.

Lengyel, Katalina; Lang, Elke; Fodor, Anna; Szallas, Emilia; Schümann, Peter; Stackebrandt, Erko

doi:10.1016/j.syapm.2004.10.004

Cited by 98 publications

(92 citation statements)

References 22 publications

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“…Because nonbiological factors such as geographic isolation of hosts and/or microbes can give the appearance of microbe-host specificity where it does not exist, we sought to experimentally establish that this interaction is, in fact, species specific. We mixed S. carpocapsae nematodes with eight different Xenorhabdus species [X. nematophila, X. beddingii, X. bovienii (ATCC 49109), X. bovienii (Jollieti), X. budapestensis, X. ehlersii, X. innexi, X. poinarii, and X. szentirmaii], including two X. bovienii isolates from distinct nematode species (4,11,20,33). Except for X. szentirmaii and X. bovienii (Jollieti), which did not support nematode development and reproduction, all species tested supported S. carpocapsae development into adults that produced progeny.…”

Section: Resultsmentioning

confidence: 99%

The Xenorhabdus nematophila nilABC Genes Confer the Ability of Xenorhabdus spp. To Colonize Steinernema carpocapsae Nematodes

Cowles

Goodrich-Blair

2008

J Bacteriol

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Members of the Steinernema genus of nematodes are colonized mutualistically by members of the Xenorhabdus genus of bacteria. In nature, Steinernema carpocapsae nematodes are always found in association with Xenorhabdus nematophila bacteria. Thus, this interaction, like many microbe-host associations, appears to be species specific. X. nematophila requires the nilA, nilB, and nilC genes to colonize S. carpocapsae. In this work, we showed that of all the Xenorhabdus species examined, only X. nematophila has the nilA, nilB, and nilC genes. By exposing S. carpocapsae to other Xenorhabdus spp., we established that only X. nematophila is able to colonize S. carpocapsae; therefore, the S. carpocapsae-X. nematophila interaction is species specific. Further, we showed that introduction of the nilA, nilB, and nilC genes into other Xenorhabdus species enables them to colonize the same S. carpocapsae host tissue that is normally colonized by X. nematophila. Finally, sequence analysis supported the idea that the nil genes were horizontally acquired. Our findings indicate that a single genetic locus determines host specificity in this bacteria-animal mutualism and that host range expansion can occur through the acquisition of a small genetic element.Microbial associations with plant and animal hosts are ubiquitous in nature and tend to show specificity with regard to the taxonomy of each partner. For example, Salmonella enterica serotype Typhi (29), Helicobacter pylori (5), Neisseria gonorrhoeae (28), and Haemophilus influenzae (22) are pathogens with specificities for human or primate hosts, while Rhizobium leguminosarum forms mutualistic nodules only with Phaseolae legumes (30). The molecular basis underlying host range specificity is well understood in the mutualism between the Rhizobiaceae and their plant hosts: specificity is achieved by cross talk involving bacterial and plant signaling factors and receptors (15). In contrast, species-specific interactions between bacteria and animal hosts are not well understood at the molecular or genetic level. Although myriad traits have been proposed to contribute to host range specificity (16,21,36), none of these have been directly demonstrated to play a role in dictating the animal host range. The increasing global concern over new human pathogens emerging through host range expansion makes understanding the genetic basis of host range specificity in bacteria-animal associations essential (6, 27).Insect parasitic nematodes of the genus Steinernema are mutualistically associated with bacteria of the Xenorhabdus genus. This association is a natural and tractable model for understanding the ecology, evolution, and molecular foundations of bacterial interactions with animal hosts. The soil-inhabiting infective stage of a Steinernema nematode is colonized by symbiotic Xenorhabdus bacteria, which it carries and releases into an insect host. Xenorhabdus bacteria provide activities that suppress insect immunity, kill the insect, and enzymatically degrade the cadaver to support nematode ...

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Section: Resultsmentioning

confidence: 99%

The Xenorhabdus nematophila nilABC Genes Confer the Ability of Xenorhabdus spp. To Colonize Steinernema carpocapsae Nematodes

Cowles

Goodrich-Blair

2008

J Bacteriol

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“…T EPB törzs XAMP-termelő primer variánsából készült el a sejtmentes fermentlé és a tisztított biopreparátum, amely a korábbi vizsgálatokban a legmagasabb antimikrobiális hatá-sú anyag termelésére volt képes [5].…”

Section: Módszerunclassified

A Xenorhabdus budapestensis entomopatogén baktérium sejtmentes fermentlevének és tisztítottfehérje-frakciójának antimikrobiális hatása néhány zoonoticus baktériumra

et al. 2015

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Bevezetés: A folyamatosan megjelenő multirezisztens kórokozók és a bevetett új antibiotikumok között állandó "harc" folyik. A klinikumban és az állatgyógyászatban használt antibiotikumok jelentős része már nem hatékony. Eltérő hatásmechanizmusú antibakteriális peptidek alkalmazása alternatív megoldást jelenthet. A rovarpatogén Xenorhabdus budapestensis baktérium szintetizálni képes különböző antimikrobiális hatású fehérjekomponenseket (bicornutin-A, fabclavin). Célkitűzés: Xenorhabdus budapestensis antibakteriális hatásának mérése zoonózist okozó baktériumokon in vitro. Módszer: Gram-pozitív (Rhodococcus equi, Erysipelothrix rhusiopathia, Staphylococcus aureus, Streptococcus equi, Corynebacterium pseudotuberculosis, Listeria monocytagenes) és Gram-negatív (Salmonella gallinarum, Salmonella derbi, Bordatella bronchoseptica, Escherichia coli, Pasteurella multocida, Aeromonas hydrophila) tesztbaktériumo-kon sejtmentes fermentlé és a tisztítottfehérje-biopreparátum antibakteriális hatásának mérése agardiffúzióval véres agaron. Eredmények: A Xenorhabdus budapestensis fehérjéket termel, amelyek hígításaitól függően erős antibakteriális aktivitást fejtettek ki a tesztelt baktériumokon. A tisztított frakció hatása erősebb volt, mint a sejtmentes fermentlé. A Gram-pozitív baktériumok érzékenyebbek voltak. Következtetések: A Xenorhabdus budapestensis antimikrobiális hatású fehérjéi hatékonyságot mutattak a zoonózist okozó baktériumokon, és potenciális esélyük lehet e szervezetek elleni bevetésre, illetve azok megelőzésében a jövőben. Orv. Hetil., 2015, 156(44), 1782-1786. Kulcsszavak: Xenorhabdus budapestensis, fabclavin, bicornutin-A, zoonózis, antimikrobiális hatásXenorhabdus budapestensis entomopathogenic bacteria cell free conditioned medium and purifi ed peptide fraction effect on some zoonotic bacteria Introduction: Many multi-resistant patogens appear continuously resulting in a permanent need for the development of novel antibiotics. A large number of antibiotics introduced in clinical and veterinary practices are not effective. Antibacterial peptides with unusual mode of action may represent a promising option against multi-resistant pathogens. The entomopathogenic Xenorhabdus budapestensis bacteria produce several different antimicrobial peptides compounds such as bicornutin-A and fabclavin. Aim: The aim of the authors was to evaluate the in vitro antibacterial effect of Xenorhabdus budapestensis using zoonotic patogen bacteria. Method: Cell-free conditioned media and purifi ed peptide fractions of Xenorhabdus budapestensis were tested on Gram-positive (Rhodococcus equi, Erysipelothrix rhusiopathia, Staphylococcus aureus, Streptococcus equi, Corynebacterium pseudotuberculosis, Listeria monocytage-

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“…This nematode infects older mole cricket nymphs and adults and recycles within the soil environment. The nematode, once inside the mole cricket host, releases Xenorhabdus innexi bacterium into the hemolymph (Lengyel et al 2005). The bacterium reproduces and kills the mole cricket through septicemia, producing a nutrient rich bacterial soup that the nematodes consume.…”

Section: R Esumenmentioning

confidence: 99%

Integration of Insect Parasitic Nematodes (Rhabditida Steinernematidae) with Insecticides for Control of Pest Mole Crickets (Orthoptera: Gryllotalpidae: <I>Scapteriscus</I> spp.)

Barbara

Buss

2005

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Description of four novel species of Xenorhabdus, family Enterobacteriaceae: Xenorhabdus budapestensis sp. nov., Xenorhabdus ehlersii sp. nov., Xenorhabdus innexi sp. nov., and Xenorhabdus szentirmaii sp. nov.

Cited by 98 publications

References 22 publications

The Xenorhabdus nematophila nilABC Genes Confer the Ability of Xenorhabdus spp. To Colonize Steinernema carpocapsae Nematodes

The Xenorhabdus nematophila nilABC Genes Confer the Ability of Xenorhabdus spp. To Colonize Steinernema carpocapsae Nematodes

A Xenorhabdus budapestensis entomopatogén baktérium sejtmentes fermentlevének és tisztítottfehérje-frakciójának antimikrobiális hatása néhány zoonoticus baktériumra

Integration of Insect Parasitic Nematodes (Rhabditida Steinernematidae) with Insecticides for Control of Pest Mole Crickets (Orthoptera: Gryllotalpidae: <I>Scapteriscus</I> spp.)

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