Description of Lelenchus brevislitus n. sp. (Nematoda: Tylenchidae), an example of a cryptic species from Iran and its phylogenetic relationships with other species in the family

Soleymanzadeh, Mahya; Pedram, Majid; Pourjam, Ebrahim; Álvarez-Ortega, Sergio

doi:10.1163/15685411-00003010

Cited by 18 publications

(8 citation statements)

References 26 publications

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“…Many species are poorly described because of an overreliance on morphological characters using light microscopy (LM) and a limited number of specimens which has led to taxonomic confusion (Qing and Bert, 2018). Recently, molecular data have been used to improve taxonomic studies of the family members (Palomares-Rius et al, 2009;Bert et al, 2010;Atighi et al, 2013;Panahandeh et al, 2014Panahandeh et al, , 2015aPanahandeh et al, , 2015bPanahandeh et al, , 2016Panahandeh et al, , 2018Soleymanzadeh et al, 2016;Qing et al, 2016Qing et al, , 2017Pedram et al, 2018;Bert, 2017, 2018). Unfortunately, molecular data are often not available for all genera and species, and some genera are only rarely recovered after their original descriptions.…”

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“…During our extensive study of tylenchids in northern Iran (Panahandeh et al, 2014(Panahandeh et al, , 2015a(Panahandeh et al, , 2015b(Panahandeh et al, , 2016Soleymanzadeh et al, 2016;Mobasseri et al, 2017;Konani et al, 2018;Pedram et al, 2018), one population of the genus Labrys representing the second species of the genus was recovered from a soil sample of the natural forests in Gilan province. It is characterized using molecular and morphological characters and described herein as L. filiformis n. sp.…”

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Morphological and Molecular Characterization of Labrys filiformis n. sp. (Rhabditida: Tylenchidae) from Iran

Panahandeh

Abolafia

Pourjam

et al. 2018

Journal of Nematology

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Labrys filiformis n. sp., the second species of the rare genus Labrys, was recovered from natural forests of Gilan province and is described based upon morphological and molecular characters. The new species is characterized by its smooth cuticle under light microscopy, lateral field with two incisures forming a single plain band, lip region continuous with body contour, dorso-ventrally flattened and forming four poorly prominent lobes, having a dorso-ventrally narrower protuberant labial plate laterally extended to the amphidial margins, oral area (oral plate) dorso-ventrally elongated and embedded in the labial plate with six small labial sensilla surrounding the slightly prominent oral aperture, amphidial apertures as longitudinally lemniscatic slits bordered by the labial plate extensions which are overlapped at the middle length of amphids, stylet delicate, 6 to 7 µm long, elongate weakly developed fusiform median bulb with weak valve, wide excretory pore with long and heavily sclerotized duct, offset spermatheca filled with small spheroid sperm cells, 106 to 127 µm long elongate-conoid tail with filiform distal region and finely rounded tip. Molecular phylogenetic analyses were performed using a near-full length fragment of the 18S rDNA and the D2-D3 expansion segments of the 28S rDNA using Bayesian inference and maximum likelihood methods. In the inferred phylogenetic tree with 18S rDNA, the new species has a close affinity with several isolates of the type species, Labrys chinensis. The reconstructed phylogenetic tree using partial 28S rDNA, revealed the new species is nested inside the putative monophyletic group of several populations of L. chinensis.

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Morphological and Molecular Characterization of Labrys filiformis n. sp. (Rhabditida: Tylenchidae) from Iran

Panahandeh

Abolafia

Pourjam

et al. 2018

Journal of Nematology

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“…In the BI tree (Figure ), the two isolates of the new species are strongly (BPP = 100) supported as a sister taxon of a clade formed by S. arboris (MF599082) + two species representing the genus Lelenchus viz. L. leptosoma (de Man, 1880) Andrássy, 1954, accession numbers , , and L. brevislitus Soleymanzadeh, Pedram, Pourjam, & Álvarez‐Ortega, 2016, accession number . On the other hand, in the ML tree (Figure ), the new species was supported (BS = 65%) as a sister taxon of S. arboris , and consequently representing the monophyly of the genus Sakia.…”

Section: Resultsmentioning

confidence: 99%

An integrative study of Sakia sisanganensis n. sp. (Rhabditida: Tylenchidae) from Sisangan forest, Iran, and new morphological observations for the genus

et al. 2019

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Further data on the morphology (the lip region characters) and phylogeny of the genus Sakia are presented. The new observations were based on scanning electron microscopy (SEM) and light microscopy (LM). A new species, Sakia sisanganensis n. sp., was recovered from rotten wood of a dead beech tree (Fagus orientalis) in northern Iran, herein described and illustrated based on an integrative approach, that is morphological, morphometric and molecular characters. The new species is characterized by a combination of the following features: fine transverse striae and vestigial single band in the lateral field in SEM. Labial area dorso-ventrally flattened. Oral region with two concentric hexagonal plates, the inner one apparently elevated. Amphidial openings short, slit-like. Stylet delicate. Median bulb fusiform to spindle-shaped with weak valvular apparatus. Spermatheca functional. Tail filiform with faintly pointed tip and males common. The new species was morphologically compared with four known species of the genus, viz., S. alii, S. arboris, S. castori and S. indica, all having indistinct lateral fields. Molecular phylogenetic analyses were performed based on the small subunit ribosomal RNA gene (SSU rDNA). In the Bayesian inference (BI),S. sisanganensis n. sp. with two isolates was strongly supported as a sister taxon of a clade harbouring S. arboris + Lelenchus species. However, in the maximum likelihood (ML) analysis, the new species formed a clade with S. arboris, thus supporting the reciprocal monophyly of the genera Sakia and Lelenchus. Accordingly, the test of monophyly was performed (using Bayes factor) and the results did not reject the monophyly of sakia (i.e., S. sisanganensis n. sp. and S. arboris as sister taxa) based on the currently available data. K E Y W O R D SBeech tree, Fagus orientalis, Iran, Mazandaran province, phylogeny, rotten wood, taxonomy 2 of 12 | PANAHANDEH Et Al.

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“…Adequacy of the posterior sample size was evaluated using autocorrelation statistics as implemented in Tracer v.1.6 ( Rambaut and Drummond, 2009 ). Aphelenchid and neotylenchid outgroup taxa were used for SSU and LSU phylogenies, respectively, based on previous studies ( Yaghoubi et al., 2016 ; Soleymanzadeh et al, 2016 ; Pereira et al., 2017 ) (species names and accession numbers listed in corresponding trees). The output ﬁles of the phylogenetic programs used herein were visualized using Dendroscope v.3.2.8 ( Huson and Scornavacca, 2012 ) and re-drawn in CorelDRAW software version 2018.…”

Section: Methodsmentioning

confidence: 99%

Cephalenchus driekieae n. sp. (Nematoda: Tylenchidae) from South Africa, a new member of the genus with a long pharyngeal overlap

Rashidifard

Preez

Abolafia

et al. 2020

Journal of Nematology

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Cephalenchus driekieae n. sp. is described and illustrated based on its morphological, morphometric, and molecular characteristics. This new species is mainly characterized by its short stylet 11.5 to 13.0 μm, and 13.5 to 17.5 μm long pharyngeal overlap extending over the intestine. It could further be delimited by 451 to 526 μm long females with a prominently annulated cuticle, dorso-ventral amphidial openings as shown using scanning electron microscopy (SEM), four lines in the lateral field, anchor-shaped stylet knobs, empty spermatheca, elongate conoid tail with finely rounded tip and males absent. The shortest stylet and long pharyngeal overlap, distinguish this new species from previously described members and update the characteristics of the genus. With four lines in the lateral field, this new species was morphologically compared with four previously described species with this feature and another species with a short stylet. Molecular phylogenetic analyses using the partial small and large subunit ribosomal DNA gene (SSU and LSU rDNA D2-D3) sequences showed that it was clustered with other Cephalenchus spp. in both SSU and LSU trees, retaining the monophyly of the genus. This new species from South Africa updates the biogeography of the genus.

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Description of Lelenchus brevislitus n. sp. (Nematoda: Tylenchidae), an example of a cryptic species from Iran and its phylogenetic relationships with other species in the family

Cited by 18 publications

References 26 publications

Morphological and Molecular Characterization of Labrys filiformis n. sp. (Rhabditida: Tylenchidae) from Iran

Morphological and Molecular Characterization of Labrys filiformis n. sp. (Rhabditida: Tylenchidae) from Iran

An integrative study of Sakia sisanganensis n. sp. (Rhabditida: Tylenchidae) from Sisangan forest, Iran, and new morphological observations for the genus

Cephalenchus driekieae n. sp. (Nematoda: Tylenchidae) from South Africa, a new member of the genus with a long pharyngeal overlap

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