Description of Lutzomyia (Helcocyrtomyia) tolimensis, a new species of phlebotomine sandfly (Diptera: Psychodidae) from Colombia

Carrasquilla, María Cristina; Munstermann, Leonard E.; Marín, Dairo; Ocampo, Clara B.; Ferro, C.

doi:10.1590/s0074-02762012000800005

Cited by 5 publications

(4 citation statements)

References 9 publications

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“…Approximately 500 species of phlebotomine sand flies have been recorded from the Americas, of which at least 164 are known to exist in Colombia, widely distributed in a variety of ecological environments [ 4 - 6 ]. In Colombia, thirteen species of Lutzomyia are associated or implicated with the transmission of different Leishmania species [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Study on natural breeding sites of sand flies (Diptera: Phlebotominae) in areas of Leishmania transmission in Colombia

et al. 2015

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BackgroundThe location of the microhabitats where immature phlebotomine sand flies of the genus Lutzomyia develop is one of the least-known aspects of this group of medically important insects. For this reason strategies of source reduction approach for their control have not been possible in contrast to other insect vectors (such as mosquitoes), because their juvenile stages in terrestrial microhabitats is difficult to detect.MethodsDirect examination of soil samples, incubation of substrates and the use of emergence traps were the methods used to identify juvenile stages in 160 soil samples from urban and forest habitats within the foci of Leishmania transmission in Colombia. Immatures collected were identified subsequent from the rearing and emergence of adults using taxonomic keys or the analysis of the mitochondrial marker cytochrome oxidase I. Plant species associated with the natural breeding sites were identified and physicochemical properties of the soils were analyzed.ResultsA total of 38 (23.7%) sampling sites were identified as breeding sites, 142 phlebotomine sand flies were identified, belonging to 13 species of the genus Lutzomyia and two of Brumptomyia. The greatest numbers of immature were found within the tabular roots (51 immature sand flies from eight positive sites) and bases of trees (35 immature sand flies from 11 sites). The characterization and presence of the tree species (mainly Ceiba pentadra, Anacardium excelsum, Pseudosamanea guachapale) and the physicochemical properties (relative humidity and carbon/nitrogen ratio) of the soils associated with these breeding sites are significant factors in explaining the diversity and abundance of phlebotomine sand flies.ConclusionsImmature phlebotomine sand flies of the genus Lutzomyia in Colombia can be found in a wide variety of breeding sites rich in organic matter, high relative humidity and are associated with a typical vegetation of each locality. These results provide new perspectives for the study of the ecology of the genus Lutzomyia in Colombia and the development of vector control strategies.

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Section: Introductionmentioning

confidence: 99%

Study on natural breeding sites of sand flies (Diptera: Phlebotominae) in areas of Leishmania transmission in Colombia

et al. 2015

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“…This list was obtained from taxonomic studies conducted in Colombia in different natural and endemic environments, with a primary focus on areas of leishmaniasis transmission [10]–[19].…”

Section: Introductionmentioning

confidence: 99%

“…To date, in Colombia, 163 species of sand flies (153 Lutzomyia , 8 Brumptomyia , and 2 Warileya species) have been recorded. This list was obtained from taxonomic studies conducted in Colombia in different natural and endemic environments, with a primary focus on areas of leishmaniasis transmission [10] – [19] .…”

Section: Introductionmentioning

confidence: 99%

DNA Barcoding for the Identification of Sand Fly Species (Diptera, Psychodidae, Phlebotominae) in Colombia

et al. 2014

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Sand flies include a group of insects that are of medical importance and that vary in geographic distribution, ecology, and pathogen transmission. Approximately 163 species of sand flies have been reported in Colombia. Surveillance of the presence of sand fly species and the actualization of species distribution are important for predicting risks for and monitoring the expansion of diseases which sand flies can transmit. Currently, the identification of phlebotomine sand flies is based on morphological characters. However, morphological identification requires considerable skills and taxonomic expertise. In addition, significant morphological similarity between some species, especially among females, may cause difficulties during the identification process. DNA-based approaches have become increasingly useful and promising tools for estimating sand fly diversity and for ensuring the rapid and accurate identification of species. A partial sequence of the mitochondrial cytochrome oxidase gene subunit I (COI) is currently being used to differentiate species in different animal taxa, including insects, and it is referred as a barcoding sequence. The present study explored the utility of the DNA barcode approach for the identification of phlebotomine sand flies in Colombia. We sequenced 700 bp of the COI gene from 36 species collected from different geographic localities. The COI barcode sequence divergence within a single species was <2% in most cases, whereas this divergence ranged from 9% to 26.6% among different species. These results indicated that the barcoding gene correctly discriminated among the previously morphologically identified species with an efficacy of nearly 100%. Analyses of the generated sequences indicated that the observed species groupings were consistent with the morphological identifications. In conclusion, the barcoding gene was useful for species discrimination in sand flies from Colombia.

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“…Además, se dispone de trabajos como el publicado por Bejarano (16), quien presentó en el 2006 el listado de la familia Psychodidae para Colombia. A la fecha se registran en el país 163 especies de flebotomíneos: 153 del género Lutzomyia, ocho del género Brumptomyia y dos del género Warileya (17)(18)(19)(20)(21). Estas especies se encuentran distribuidas en diversos ecosistemas del país, que van desde el bosque muy húmedo tropical (bmh-t) hasta el bosque seco tropical (BsT), y muchas se registran en ambientes de influencia antrópica, como los sistemas agropecuarios y los ambientes urbanos (22,23).…”

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Lista actualizada de flebotomíneos (Diptera: Psychodidae: Phlebotominae) de la región cafetera colombiana

et al. 2014

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Description of Lutzomyia (Helcocyrtomyia) tolimensis, a new species of phlebotomine sandfly (Diptera: Psychodidae) from Colombia

Cited by 5 publications

References 9 publications

Study on natural breeding sites of sand flies (Diptera: Phlebotominae) in areas of Leishmania transmission in Colombia

Study on natural breeding sites of sand flies (Diptera: Phlebotominae) in areas of Leishmania transmission in Colombia

DNA Barcoding for the Identification of Sand Fly Species (Diptera, Psychodidae, Phlebotominae) in Colombia

Lista actualizada de flebotomíneos (Diptera: Psychodidae: Phlebotominae) de la región cafetera colombiana

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