Description of Meiosis in Female and Male Pacific White Shrimp Litopenaeus vannamei (Decapoda: Penaeidae)

Garza‐Torres, Rodolfo; Maeda-Martínez, Alejandro M.; Guerrero‐Tortolero, Danitzia A.; Obregón-Barboza, Hortencia; Campos‐Ramos, Rafael

doi:10.1651/10-3316.1

Cited by 8 publications

(4 citation statements)

References 40 publications

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“…The spermatocytogenesis produces haploid spermatids from diploid spermatogonia. Spermiogenesis is the process that transforms the mature spermatids into spermatozoa [ 69 ]. Due to the juvenile testis sample containing most spermatogonia and primary spermatocyte to transform into spermatid, many expressed genes were found.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of banana shrimp (Fenneropenaeus merguiensis) ovaries and testes: Insights into FoxL2

Potiyanadech,

Choomee,

Chotigeat

2023

PLoS ONE

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The banana shrimp is found in the Pacific and Indian Oceans. Female shrimp are preferred for consumption because they are larger than males. Understanding the mechanism of sex differentiation is important for developing techniques to increase the number of female shrimp for economic benefits. This study investigates the reproductive development of F. merguiensis using transcriptome analysis. Sxl2, dsx, AGH, FEM-1, and Nrg-X2 were classified as essential genes for testes development during the juvenile stage. Several genes were required for both juvenile and adult male development. Additionally, the expression of several genes was shown to be required for juvenile and adult ovarian development, including SOP1, SOP2, Ptgr1, EST, Vgr, Vmol1, and TR-beta A. Interestingly, high levels of FoxL2 expression were observed in the testes, in contrast to previous studies in humans and other mammals. The binding of FoxL2 to the Vtg promoter was demonstrated in silico with the highest relative binding score (RS = 0.89) using the JASPAR program. Knock-down of the FoxL2 gene with dsRNA significantly suppressed FoxL2 at 2, 4, and 6 d. As a result, Vtg expression increased when compared with the control at 2, 4, and 6 d, indicating that FoxL2 plays an important role in Vtg expression in the ovary. Our findings highlight the role of FoxL2 in banana shrimp reproduction and provide valuable information on the genes associated with the F. merguiensis reproductive system.

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Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of banana shrimp (Fenneropenaeus merguiensis) ovaries and testes: Insights into FoxL2

Potiyanadech,

Choomee,

Chotigeat

2023

PLoS ONE

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“…Spermatogenesis is the process of haploid spermatozoa production and is divided into two stages: spermatogenesis and spermiogenesis. Spermatogenesis is the process of diploid spermatogonia and haploid spermatid production, and spermiogenesis is transforming mature spermatids into spermatozoa [ 1 ]. Many studies in crustaceans have focused on spermiogenesis because the most representative process data are available for each species, including Sicyonia ingentis [ 2 ], Parapenaeus longirostris [ 3 ], Macrobrachium rosenbergii [ 4 ], Fenneropenaeus chinensis [ 5 ], Litopenaeus vannamei [ 6 ], and Penaeus monodon [ 7 ] However, spermatogenesis in Fenneropenaeus merguiensis is still unknown.…”

Section: Introductionmentioning

confidence: 99%

Differentially expressed genes in the testes from early to mature development of banana shrimp (Fenneropenaeus merguiensis)

Saetan,

Chotigeat

2023

PLoS ONE

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Banana shrimp (Fenneropenaeus merguiensis) is an economically important species in Thailand owing to the high value of globally exported frozen brine shrimps. However, the regulatory mechanisms governing spermatogenesis and testicular development in this species are poorly understood. High-throughput RNA sequencing was used to investigate the mechanisms and regulated genes involved in testis development using transcriptome profiling of juvenile and adult banana shrimp testes. Differentially expressed genes (DEGs) in these two libraries were identified and quantified to confirm gene expression. DEGs were found in 7,347 genes, with 4,465 upregulated and 2,882 downregulated. Some of these genes were designated as candidate genes, and six specific DEGs, including PRM1, SPATA20, Sry, SSRF, Sxl, and Tra-2c, were selected to confirm the reliability of the RNA-seq data using qPCR. Moreover, six non-DEGs were chosen based on testis-specific and regulatory genes that support a specific function in spermatogenesis and testis development in this species, including Dsx, Gfra2, IAG, Sox9, Sox13, and Sox14A. Furthermore, Sry, Sox14A, Sox14B and SPATA20 were identified in early stages (nauplius-postlarvae) of shrimp development to provide more information involving testes formation and development. The transcript data from this study could differentiate a group of genes required at the early and late stages of testis development and both sets of testis development. Therefore, this information would help in manipulating each stage of testicular development.

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“…The majority of studies about meiosis conducted so far using obligate sexual crustaceans have used classical histological techniques, or orcein staining, and have rarely used electron microscopy. Studies using male marine shrimps Fenneropenaeus chinensis (Xie et al 2008 ) and Litopenaeus vannamei (Garza-Torres et al 2011 ; Heitzmann et al 1993 ) concluded that spermatogenesis is a continuous process, unrelated to the crustacean moult cycle. Cytogenetic studies using male brine shrimps Artemia franciscana (Papeschi et al 2000 ) and A. persimili (Rodriguez-Gil et al 1998 ) were limited to describing the first stages of male meiosis (no stages further than diakinesis).…”

Section: Introductionmentioning

confidence: 99%

Male meiosis in Crustacea: synapsis, recombination, epigenetics and fertility in Daphnia magna

et al. 2015

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We present the first detailed cytological study of male meiosis in Daphnia (Crustacea: Branchiopoda: Cladocera)—an aquatic microcrustacean with a cyclical parthenogenetic life cycle. Using immunostaining of the testes in Daphnia magna for baseline knowledge, we characterized the different stages of meiotic division and spermiogenesis in relation to the distribution of proteins involved in synapsis, early recombination events and sister chromatid cohesion. We also studied post-translational histone modifications in male spermatocytes, in relation to the dynamic chromatin progression of meiosis. Finally, we applied a DNA fragmentation test to measure sperm quality of D. magna, with respect to levels of inbreeding. As a proxy for fertility, this technique may be used to assess the reproductive health of a sentinel species of aquatic ecosystems. Daphnia proves to be a model species for comparative studies of meiosis that is poised to improve our understanding of the cytological basis of sexual and asexual reproduction.Electronic supplementary materialThe online version of this article (doi:10.1007/s00412-015-0558-1) contains supplementary material, which is available to authorized users.

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Description of Meiosis in Female and Male Pacific White Shrimp Litopenaeus vannamei (Decapoda: Penaeidae)

Cited by 8 publications

References 40 publications

Transcriptome profiling of banana shrimp (Fenneropenaeus merguiensis) ovaries and testes: Insights into FoxL2

Transcriptome profiling of banana shrimp (Fenneropenaeus merguiensis) ovaries and testes: Insights into FoxL2

Differentially expressed genes in the testes from early to mature development of banana shrimp (Fenneropenaeus merguiensis)

Male meiosis in Crustacea: synapsis, recombination, epigenetics and fertility in Daphnia magna

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